Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,456,444 C→T A23V (GCT→GTT)  gspD → Type II secretion system protein GspD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,456,4440CT93.3% 30.4 / ‑5.3 15A23V (GCT→GTT) gspDType II secretion system protein GspD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (7/7);  minor base G (0/1);  total (7/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01

GATCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTCCGTCGGGGAACTTACAGGAATGAATGGACTGCGTCATGAAAGGACTCAATAAAATCACCTGCTGCTTGCTGGCAGCACTACTCATGCCTTGTGCAGGACACGCTGAGAACGAACAATACGGCGCGAACTTCAATAACGCCGATATCCGCCAGTTCGTGGAAATAGTGGGTCAGCATCTTGGCAAAACGATCCTGATCGACCCTTCGGTACAGGGAACCATTTCCGTACGCAGTAATGATACGTTTAGCCAACAGGAGTACTACCAGTTCTTTTTAAGTATTCTTGATCTTTACGGTTATTCCGTGATCACGCTGGACAATGGTTTTCTGAAAGTGGTTCGCTC  >  NC_000913/3456219‑3456684
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                
gATCAATAAGCTTTCGCTGACCACCCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACTCCGCTTGATAAATGTTGCCGTCGGGGAACTTACAGGAATGAATGGACTGCGTCATGTAAGGACTCAATAAAAGCTCCTGCTGCTTGCTTGCAGCACTACTCATGCCTTGTTCAGGACACGGTGAGAACGAACAATACGGCGCGAAc                                                                                                                                                                                                                         <  1:239635/251‑1 (MQ=255)
                                         aGGCATTAAGTTTACTGCTAACTCAACATATTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTCCGTCGGGGAACTTACAGGAATGAATGGACTGCGTCATGAAAGGACTCAATAAAATCACCTGCTGCTTGCTGGCAGCACTACTCATGCCTTGTGCAGGACACGTTGAGAACGAACAATACGGCGCGAACTTCAATAACGCCGATATCCGCCAGTTCGTGGAAATAGTggg                                                                                                                                                                                <  1:107100/251‑1 (MQ=255)
                                                  gTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTCCGTCGGGGAACTTACAGGAATGAATGGACTGCGTCATGAAAGGACTCAATAAAATCACCTGCTGCTTGCTGGCAGCACTACTCATGCCTTTTGCTGGACACGTTGAGAACTAACAATACgg                                                                                                                                                                                                                                <  1:259403/194‑1 (MQ=255)
                                                                                   cAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTACGTCGGGGAACTTACAGGAATGAATGGCCTGCGTCATGAAAGGACTCAATAAAATCACCTGCTGCTTGCTGGCAGCACTACTCATGCCTTGTGCAGGACACGTTGAgaacgaac                                                                                                                                                                                                                                       >  1:217293/1‑154 (MQ=255)
                                                                                         gtcgCAACGGCGTCCCCCGCTTGATAAATGTTTCCGTCGGGGAACTTACAGGAATGAATGGACTGCGTCATGAAAGGACTCAATAAAATCACCTGCTGCTTGCTGGCAGCACTCCTCATGCCTTGTGCAGGACACGTTGAGAACGAACAATACGGCGCGAACTTCAATAACGCCGATATCCGCCAGTTCGTGGAAATAGTGGGTCAGCATCt                                                                                                                                                                       >  1:260207/1‑212 (MQ=255)
                                                                                            gCAACGGCGTACCCCGCTTGATAAATGTTTCCGTCGGGGAACTTACAGGAATGAATGGACTGCGTCATGAAAGGACTCAATAAAATCACCTGCTGCTTGCTGGCAGCACTACTCATGCCTTGTGCAGGACACGTTGAGAACGAACAATACGGCGCGAACTTCAATAACGCCGATATCCGCCAGTTCGTGGAAATAGTGGGTCAGCATCTTGGCAAAACGATCCTGATCGACCCTTCGGTACAGGGAACCAt                                                                                                                             >  1:88083/1‑251 (MQ=255)
                                                                                                                                                  atgGACTGCGTCATGAAAGGACTCAATAAAAGCACCTGCTGCTTGCTGGCAGCACTACTCATGCCTTGTGCAGGACACGTTGAGAACGAACAATACGGCGCGAACTTCAATAACGCCGATATCCGCCAGTTCGTGGAAATAGTGGGTCAGCATCTTGGCAAAACGATCCTGATCGACCCTGCGGTACAGGGAACCATTTCCGTACGCAGTAATGATACGTTTAGCCAACAGTAGTACTAGCAGTTCttttt                                                                       <  1:112997/251‑1 (MQ=255)
                                                                                                                                                       cTGCGTCATGAAAGGACTCAATAAAATCACCTGCTGCTTGCTGGCAGCACTACTCATGCCTTGTGCAGGACACGTTGAGAACGAACAATACGGCGCGAACTTCAATAACGCCGATATCCGCCAGTTCGTGGAAATAGTGGGTCAGCATCTTGGCAAAACGATCCTGATCGACCCTTCGGTACAGGGAACCATTTCCGTACGCAGTAATGATACGTTTAGCCAACAGGAGTACTACCAGTTCTTTTTAAGt                                                                   >  1:10540/1‑250 (MQ=255)
                                                                                                                                                                gAAAGGACTCAATAAAATCACCTGCTGCTTGCTGGCAGCACTACTCATGCCTTGTGCAGGACACGTTGAGAACGAACAATACGGCGCGAACTTCAATAACGCCGATATCCGCCAGTTCGTGGAAATAGTGGGTCAGCATCTTGGCAAAACGATCCGGATCGGCCCTGCGGTACAGGGAACCATGTCCGTACGCAGTAATGATACGTTTAGCCAACAGGAGTACTACCAGTTCTTTTTAAGTATTCTTGATc                                                         <  1:322582/251‑1 (MQ=255)
                                                                                                                                                                      gttccgaccttccgttttcccgtctctGGCAGCACTTATCAATCCTTGTGCAAGACACGTTGAGAACGAAAAAAACGGCCGGAAATTCAATAACGCCGATAACCGCCAGTTCGTGGGAATAGTGGGTCAGCATCTTGGCAAAACGAACCCGGACGACCCTTCGGTACCGGGAACCATTTCCGTACGCAGTAATGATACGTTTAGCCAACAGGAGTACTACCAGTTCTTTTTAAGTATTCTTGATCTTTACg                                                   <  1:11358/226‑1 (MQ=255)
                                                                                                                                                                                          gcttgctGGCAGCACTACTCATGCCTTGTGCAGGACACGTTGAGAACGAACAATACGGCGCGAACTTCAATAACGCCGATATCCGCCAGTTCGTGGAAATAGTGGGTCAGCATCTTGGCAAAACGATCCTGATCGACCCTTCGGTACAGGGAACCATTTCCGTACGCAGTAATGATACGTTTAGCCAACAGGAGTGCTACCAGTTCTTTTTAAGTATTCTTGATCTTTACGGTTATTCCGTGATCACGCTg                               <  1:42591/251‑1 (MQ=255)
                                                                                                                                                                                          gcttgctGGCAGCACTACTCATGCCTTGTGCAGGACACGTTGAGAACGAACAATACGGCGCGAACGTCAAGAACGCCGATATCCGCCAGTTCGTGGAAATAGTGGGTCAGCATCTTGGCAAAACGAGCCTGATCGACCCTTCGGTACAGGGAACCATTTCCGTACTCAGTAATTATACGTTTATCCAACAGGAGTACTACCAGTTCTTTTTAAGTATTCTTGATCTTTACGGTTATTCCGTGATCACGCTg                               <  1:211803/251‑1 (MQ=255)
                                                                                                                                                                                           cttgctGGCAGCACTACTCATGCCTTGTGCAGGACACGTTGAGAACGAACAATACGACGCGAACTTCAATAACGCCGATATCCGCCAGTTCGTGGAAATAGTGGGTCAGCATCTTGGCAAAACGATCCTGATCGACCCTTCGGTACAGGGAACCATTTCCGTACGCAGTAATGATACGTTTAGCCAACAGGAGTACTACCAGTTCTTTTTAAGTATTCTTGATCTTTACGGTTATTCCGTGATCACGCTgg                              >  1:186960/1‑251 (MQ=255)
                                                                                                                                                                                           cttgctGGCAGCACTACTCATGCCTCNTGCAGGNNACNTTGAGAACGAACAATACGGNGCNNNCNNNANTCACGNCGNNATCCGACAGNTCGTGGAAATAGTGGGTCAGAATCTTGGCAAAACGATCCTGATCGACCCTTCCNTACAGGGCANNATTTCCGTACGCAGTAATGATNCGTNNANNCNACa                                                                                            >  2:74332/1‑189 (MQ=255)
                                                                                                                                                                                                                       gCAGGACACGTTGAGAACGAACAATACGGCGCGAACTTCAATAACGCCGATATCCGCCAGTTCGTGGAAATAGTGGGTCAGCATCTTGGCAAACCGATCCTGATCGACCCTTCGGTACAGGGAACCATTTCCGTACGCAGTAATGATACGTTTAGCCAACAGGAGTACTACCAGTTCTTTTTAAGTATTCTTGATCTTTACGGTTATTCCGTGATCACGCTGGACACTGGTTTTCTGAACGTGGTTCGCTc  >  1:245314/1‑251 (MQ=255)
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                
GATCAATAATCTTTCGCTGACCCACCCTGATGAGGTTTCACAGGCATTAAGTTTACTGCTAACTCAACAAAGTGCTCAGTTTACAATTCGTCGCAACGGCGTACCCCGCTTGATAAATGTTTCCGTCGGGGAACTTACAGGAATGAATGGACTGCGTCATGAAAGGACTCAATAAAATCACCTGCTGCTTGCTGGCAGCACTACTCATGCCTTGTGCAGGACACGCTGAGAACGAACAATACGGCGCGAACTTCAATAACGCCGATATCCGCCAGTTCGTGGAAATAGTGGGTCAGCATCTTGGCAAAACGATCCTGATCGACCCTTCGGTACAGGGAACCATTTCCGTACGCAGTAATGATACGTTTAGCCAACAGGAGTACTACCAGTTCTTTTTAAGTATTCTTGATCTTTACGGTTATTCCGTGATCACGCTGGACAATGGTTTTCTGAAAGTGGTTCGCTC  >  NC_000913/3456219‑3456684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: