Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	603,168	C→T	Q404* (CAG→TAG)	pheP →	phenylalanine:H(+) symporter PheP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	603,168	0	C	T	100.0%	36.6 / NA	14	Q404* (CAG→TAG)	pheP	phenylalanine:H(+) symporter PheP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/9);  total (5/9)

GTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTGCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTG  >  NC_000913/602928‑603294                                                                                                                                                                                                                                                 |                                                                                                                               gtgCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTTGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGGTGCTGTTGAACGGGATTATTATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACATAGTTTAAgg                                                                                                                       <  1:138007/250‑1 (MQ=255)               gcCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACATAGTTTAAgg                                                                                                                       <  1:259951/236‑1 (MQ=255)                   aaGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACATAGTTTAAGGCGCTGCTCGATCCGTTcan                                                                                                    <  1:262227/251‑3 (MQ=255)                                                     cGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACATAGTTTAAGGCTCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGAtt                                                                  >  1:323/1‑251 (MQ=255)                                                      gATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGTTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTTGTCTGCTGATGTCGCTGGTGGTATCAACGCTGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTGTCGTGCAGCGATGCGACGTCAGGTGCGGGAAACATAGTTTAAGGCGCTGCTCTATCCgn                                                                                                        <  1:35700/212‑2 (MQ=255)                                                                          ccGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACATAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTGCACgatg                                             <  1:139636/251‑1 (MQ=255)                                                                           cGGAGCGATCACTTCGCTGGTGGAGTTAATAAACTCTCTGCTGCCGCAACAAGCGATTGGTCTGCTGATGGAGCTGGTGGTAGCACCACTGCTGTTGCACTGGATTCTGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACATAGTTTAAGGCCCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCATTCCTCGGCCTGATTTTGCTGCTGATGTGCACgatgg                                            >  1:288431/1‑251 (MQ=255)                                                                                                  tgtTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGATGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACATAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGAtgtgn                                                   <  1:84725/221‑2 (MQ=255)                                                                                                       aTCAACTATCGGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGTATTATGATCTGGCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACATAGTTTAAGGCgct                                                                                                                   <  1:156737/152‑1 (MQ=255)                                                                                                                    tgcCGCCAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACATAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATATCTGCATTGCCTTCCTCGGCCTGATTTTGCTGCTGATGTGCACGATGGATGATATGCGCTTGTAAGCGATCCTGCTGCCGGTGTGGAtt   >  1:270827/1‑251 (MQ=255)                                                                                                                                 gTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACATAGTTTAAgg                                                                                                                       <  1:783/122‑1 (MQ=255)                                                                                                                                                                                       naTCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACATAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTGCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCgg           >  1:204765/2‑176 (MQ=255)                                                                                                                                                                                                                      gCGATGCGACGTCAGGGGCGTGAAACATAGGTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTGCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGGGGGGATTg  >  1:8043/1‑154 (MQ=255)                                                                                                                                                                                                                       cGNNNNNACGNCAGGGGCGTGAAACATAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTGCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCgg           <  2:204765/144‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                               GTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGGCGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTGCACGATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTG  >  NC_000913/602928‑603294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑