Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,392,516	G→A	noncoding (45/76 nt)	glyX →	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,516	0	G	A	100.0%	27.1 / NA	13	noncoding (45/76 nt)	glyX	tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/8);  total (5/8)

CGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCC  >  NC_000913/4392319‑4392755                                                                                                                                                                                                      |                                                                                                                                                                                                                                                cgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGTGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCTCGAGTTCGCGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCGCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGAGTcgc                                                                                                                                                                                                                                            <  1:42068/203‑1 (MQ=255)                   aCGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGAGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCggg                                                                                                                                                                          <  1:239879/251‑1 (MQ=255)                                 aaGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGTCCTTGCCAAGGGCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAGATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGAGTCGCGTGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTgg                                                                                                                                                            <  1:304124/251‑1 (MQ=255)                                        acgcggtaagtgCTCAGTTTGTATAGCACGACCTTGCACAGGTGGGGGTCTCGATTGCGAGTCGCGGTTCCCGCTGCAAAATGTGAAAAGTGCTGCAAAGCACAGACTACCCAAGCGGTAATTGCTCAGTTGGTAGAGCACGCCCTTGCCAAGGTCGGAGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAGGTGAAAGTGCTGTAATGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGc                                                                                                                                                      <  1:42475/239‑1 (MQ=18)                                                 tAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTACCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCCAGGTCGGAGTCGCGAGCTCGAGTCTCGTTTCCCGATCCAAACTTTGAAAGAGCTGTAAGGCACAGACCACCCCAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTgt                                                                                                                                            >  1:311987/1‑250 (MQ=18)                                                                      aCCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGACGCCAAGCCCAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGAGTCGCGAGTTCGAATCTCGTTTCCCGCTCCAAACTTTGAAAGGGCTGTAAGGCACAGACCACCCCAGCGGGAATCGCTCAGTTGGTAGAGCAAGACCTTGc                                                                                                                                            >  1:292423/1‑230 (MQ=18)                                                                                                  gAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAACACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGCAGTCGCGCGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCAAAGCCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGCCGCGGGTTCGAGTATCGTTTCCCGCTCCCAAttcttc                                                                                           >  1:290761/1‑251 (MQ=18)                                                                                                    gTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGAGTCGCGAGGTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCan                                                                                                                                                                <  1:38071/180‑2 (MQ=255)                                                                                                                                aGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGAGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGTTCGGGGTCGCGAGTTCGAGGCTCGTTTCCCGCGCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATg                                                             <  1:167934/251‑1 (MQ=255)                                                                                                                                              catgccaccCAAGCGGGAATTGCTCAGTTGGTAGAGCACTACCTTGCCAAGCTCGGAGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGGCTCGTTGCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTga                                                    <  1:54301/242‑2 (MQ=18)                                                                                                                                                     cccAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGAGTCGCGAGTTCGAGTCGCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTTGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGGTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTttgtt                                         <  1:151281/250‑1 (MQ=255)                                                                                                                                                                                           gCCAAGGGCGGAGTCGCGAGTTCGAGTCTCGTTTTCCGCTCCAAAATTTGAAAGAGCTGTAAGACACAGACCACCCAAGCGGGAATAGCTTGGTTGGTAGAGTACGTCCTTGCCAAGGTAGGGGGCGCGAGTTCGAGTCTCGTTTCCCGCTCCACATTATTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGATTGTTTTTGTTGTCTCTGCCAAACTCTTGTTAACCGAGTTATCCACAGcc  >  1:240923/1‑251 (MQ=21)                                                                                                                                                                                            ccAAGGTCGGAGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGACAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATCGCTACGTTGGTAGAGCACGTCATTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATACACAGCGACGCGATGAGTTATTGCTGGTTTTTGTTGTCTCTGACAACCTCTTGTAAACAGAGTTATc         >  1:236120/1‑243 (MQ=21)                                                                                                                                                                                                      |                                                                                                                                                                                                                                                CGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCC  >  NC_000913/4392319‑4392755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑