Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	563,802	G→A	G158D (GGT→GAT)	sfmF →	putative fimbrial protein SfmF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	563,802	0	G	A	100.0%	30.0 / NA	12	G158D (GGT→GAT)	sfmF	putative fimbrial protein SfmF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/6);  total (6/6)

CGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGAATTACGTAATAGCGATCGCTCCCGGCTCGCACTGGGGGAGGCGAGCCCGACTGAGGAAGTAGATGCAAATGGCAATGTCACACTAAACTTTTTTGCCAATTATCGAGCGTTAGCCAGCGGTGTTCGGCCAGGTGTGGCGAAAGCGGATGCGATATTTATGATCAATTATAATTAATATTATATTAATTCGTATAATTTGGCGTAGTCGATAAGCTCTACAATTGAATGCAAACCTAGCTTGCCATAAATATTAGATTTATGCGCACTAACTGTTTTATTGCTAAGTAATAACTTATCGGCAATTTCTTTATTAGATAATCCGC  >  NC_000913/563598‑563993                                                                                                                                                                                                             |                                                                                                                                                                                                cGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGAATTACGTAATAGCGATCGCTCCCGGCTCGCACTGGGGGAAGCGAGCCCGACTGAGGAAGTAGATGCAAATGGCAATGTCACACTAAACTTTTTTGCCAATTATCGAGCGTTAGCCAGAGGTGTTCGGCCAGATGTGGCGAAAGCGGATGCGATATTTATGATCAATTATAATTAatat                                                                                                                                                   >  1:125146/1‑251 (MQ=255)                                ggCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGAATTACGTAATAGCGATCGCTCCCGGCTCGCACTGGGGGAGGCGAGCCCGACTGAGGAAGTAGATGCAAATGGCAATGTCACACTAAACTTTTTTGCCAATTATCGAGCGTTAGCCAGCGGTGTTCGGCCAGATGTGGCGAAAGCGGATGCGATAttt                                                                                                                                                                        <  1:23452/199‑1 (MQ=255)                                                                    cATCGAATTACGTAATAGCGATCGCTCCCGGCTCGCACTGGGGGAGGCGAGCCCGACTGAGGAAGTAGATGCAAATGGCAATGTCACACTAAACTTTTTTGCCAATTATCGAGCGTTAGCCAGCGGTGTTCGGCCAGATGTGGCGAAAGCGGATGCGATATTTATGATCAATTATAATTAATATTATATTAATTCGTATAATTTGGCGTAGTCGAGAAGCTCTACAATTGAATGCAAACCTAgn                                                                                       <  1:79345/244‑2 (MQ=255)                                                                                                                                          gCAAATGGCAATGTCACACTAAACTTTTTTGCCAATTATCGAGCGTTAGCCAGCGGTGTTCGGCCAGATGTGGCGAAAGCGGATGCGATATTTATGATCAATTATAATTAATATTATATTAATTCGTATAATTTGGCGTAGTCGATAATCTCTACAATTGAATGCAAACCTAGCTTg                                                                                    <  1:249658/177‑1 (MQ=255)                                                                                                                                                  cAATGTCACACTAAACTTTTTTGCCAATTATCGAGCGTTAGCCAGCGGTGTTCGGCCAGATGTGGCGAAAGCGGATGCGATACTTATGATCAATTATAATTAATATTATATTAAtt                                                                                                                                         >  1:252823/1‑116 (MQ=255)                                                                                                                                                              aaaCTTTTTTGCCAATTATCGAGCGTTAGCCAGCGGTGTTTCGCCAGATGTGTCGCCAGCGGATGCGACATTTATg                                                                                                                                                                     >  1:185141/1‑76 (MQ=255)                                                                                                                                                                 cTTTTTTTCCAATTATCGAGCGTTAGCCAGCGGTGTTCGGCCAGATGTGGCGAAAGCGGATGCGATATTTATGATCAATTATAATTAannttatat                                                                                                                                              >  2:24456/1‑96 (MQ=255)                                                                                                                                                                 cTTTTTTGCCAATTATCGAGCGTTAGCCAGCGGTGTTCGGCCAGATGTGGCGAAAGCGGATGCGATATTTATGATCAATTATAATTAATATTATATTAATTCGTATAATTTGGCGTAGTCGATAAGCTCTACAATTGAATGCAAACCTAGCTTGCCATAAATATTAGATTTATGCGCACTAACTGTTTTATTGCTAAGTAATAACTTATCGGc                         <  1:24456/213‑1 (MQ=255)                                                                                                                                                                         ccAATTATCGAGCGTTAGCCAGCGGTGTTCGGCCAGATGTGGCGAAAGCGGATGCGATATTTCTGATCAATTATACTTAATGTTATATTAATTCGTATAATTTGGCGTAGTCGATAAGCTCTACAATTGAATGCAAACCTAGCTTCCCATAAATATTAGATTTATGCGCACTAACTGTTTTATTGCTAAGTAATAACTTATCGGCAATTTCTTTATTAGATAATCCGc  >  1:247104/1‑228 (MQ=255)                                                                                                                                                                           aaTTATCGAGCGTTAGCCAGCGGTGTTCGGCCAGATGTGGCGAAAGCGGATGCGATATTTATGATCAATTATAATTAATATTATATTAATTCGTATAATTTGGCGTAGTCGATAAGCTCTACAATTGAATGCAAACCTAGCTTGCCATAAATATTAGATTTATGCGCACTAACTGTTTTATTGCTAAGTAATAACTTATCg                           <  1:223528/201‑1 (MQ=255)                                                                                                                                                                                         ngCNNGCGGNGTTCGGCCAGATGTGGCGAAAGCGNATGCGATATTTATGATCAATTATAATTAATGNTNTATTAATTCGTATAATTTGGCGTAGTCGATAAGCTCGACAATTGAATGNAAACCTAGCTNGCCNNAAATNTNANATTTATTCGCACTAACTGTTTTATTGCTANGTAATAACTTATCGGCAATTTCTNNATNNNATNNTCCGc  <  2:247104/211‑1 (MQ=255)                                                                                                                                                                                                        gCCAGATGTGGCGAAAGCGGCTGCGATATTTATGATCAATTATAATTAATATTATATTAATTCGTATAATTTGGCGTAGTCGATAAGCTCTACAATTGAATGCAAACCTAGCTTGCCATAAATATTAGATTTATGCGCACTAACTGTTTTATTg                                             >  1:252986/1‑154 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                                CGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGAATTACGTAATAGCGATCGCTCCCGGCTCGCACTGGGGGAGGCGAGCCCGACTGAGGAAGTAGATGCAAATGGCAATGTCACACTAAACTTTTTTGCCAATTATCGAGCGTTAGCCAGCGGTGTTCGGCCAGGTGTGGCGAAAGCGGATGCGATATTTATGATCAATTATAATTAATATTATATTAATTCGTATAATTTGGCGTAGTCGATAAGCTCTACAATTGAATGCAAACCTAGCTTGCCATAAATATTAGATTTATGCGCACTAACTGTTTTATTGCTAAGTAATAACTTATCGGCAATTTCTTTATTAGATAATCCGC  >  NC_000913/563598‑563993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑