Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,323,892	C→T	A149V (GCC→GTC)	atoD →	acetyl‑CoA:acetoacetyl‑CoA transferase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,323,892	0	C	T	100.0%	26.7 / NA	12	A149V (GCC→GTC)	atoD	acetyl‑CoA:acetoacetyl‑CoA transferase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

GGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGTACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACGCCCACTGCGCGCCGACCTGGCGCTAATTCGCGCTCATCGTTGCGACACACTTGGCAACCTGACCTATCAACTTAGCGCCCGCAACTTTAACCCCCTGATAGCCCTTGCGGCTGATATCACGCTGGTAGAGCCAGATGAACTGGTCGAAACCGGCGAGCTGCAACCTGACCATATTGTCACCCCTGGTGCCGTTATCGACCACATCATCGTTTCACAGGAG  >  NC_000913/2323681‑2324100                                                                                                                                                                                                                    |                                                                                                                                                                                                                 ggCACCAACCCGGAAACAGGTCGGCGCATGCTAACTGGAGAGATGGACGTCGTTCTGGTGCCGCACGGTACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACGCCCACTGCGCGTCGACCTGGCGCTAATTCGCGCTCATCGTTGCGACACACt                                                                                                                                                                           >  1:256907/1‑251 (MQ=255)             gAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGTACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACGCCCACTGCGCGTCGACCTGGCGCTAATTCGCGCTCATCGTTGCGACACACTTggn                                                                                                                                                                       <  1:206063/243‑2 (MQ=255)                          ncATGATATCTGGTGCGATGGACGTCGTTCTGGTGCCGCAAGCTACGCTAATCGAGCAAATTCGCTGTGGTGGCGCTGGACTTGGAGGTTTTCTCCCCCCAACGGGTGTCGGCACCGTCGTAGAGGACGGCAACAAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACGCCCAATACGCGTCGACCTGGCGCTCATTCGCGCTCATCGTTGCGACACACTTGGCAACCTGACCTATCAACTTAgc                                                                                                                                                  >  1:59708/2‑251 (MQ=255)                                                        tGGTGCCGCAAGGTACGCTAATCGATCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCTTAGAGGAAGGCAAACAGACACTGACACTCGACGGTAAAACCTTGCTGCTCGAACGCCCACTGCGCGTCGACCTGGCGCTAATTCGCGCTCATCGTTGCGACACACTTGGCAACCTGACCTATCAACTTAGCGccc                                                                                                                                              <  1:48513/225‑1 (MQ=255)                                                                                          cTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACGCCCACTGCGCGTCGACCTGGCGCTAATTCGCGCTCATCGTTGCGACACACTTGGCAACCTGACCTATCAACTTAGCGCCCGCAACTTTAACCCCCTGATAGCCCTTgn                                                                                                                  <  1:62474/219‑2 (MQ=255)                                                                                                        gACTTGGTGGTTTTCTCACCCCAACTGGTGTCGGCACCGTCGTAGAGGAAGGCAAACATACACTGACACTCGACTGTAAAACCTGGCTGCTCGAACGCCCACTGCGCGTCGACCTg                                                                                                                                                                                                           <  1:297289/116‑1 (MQ=255)                                                                                                                        cACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACGCCCACTGCGCGTCGACCTGGCGCTAATTCGCGCTCATCGTTGCGACACGCTTGGCAACCTGACCTATCAACTTAGCGCCCGCAACGTTAACCCCCGGATAGCCCTTGCGGCTGATATGACGCTGGTAGAGCCAGATGAACTGGTCGAAACCGGCGAGCTGCAACCTGAcc                                                    <  1:160184/251‑1 (MQ=255)                                                                                                                                             cgtcgtAGAGGAAGGCAAACAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACGCCCACTGCGCGTCGACCTGGCGCTAATTCGCGCTCATCGTTGCGACACACTTGGCAACCTGACCTATCAACTTAGCGCCCGCAACTTTAACCCCCTGATAGCCCTTGCGGCTGATATCACGCTGGTAGAGCCAGATGAACTGGTCGAAACCGGCGAGCTGCAACCTGACCATATTGTCACCCCTGGTGccn                               <  1:262255/251‑2 (MQ=255)                                                                                                                                                            cAAACAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACGCCCAATGCGCGTCGACCTGGCGCTAATTACCGCTCATCGTTGCGACACACTTGGCAACCTGACCTATAAACTTAGCGCCCGCAACTTTAACCCCCTGATAGCCCTTGCGGCTGATATCACGCTGGTAGAGCCAGATGAACTGGTCGAAACCGGCGAGCTGCAACCTGACCATATTGTCACCCCTGGTGCCGTTATCGACCAcatc                 >  1:155684/1‑250 (MQ=255)                                                                                                                                                            cAAACAGACACTGACACGCGACGGTAAAACCTGGCTGCTCGAACACCCACGGCGCGTCGACCTGGCGCTAATTCGCGCTCATCGTTGCGACACACTTGGCAACCTg                                                                                                                                                                 <  1:319973/106‑1 (MQ=255)                                                                                                                                                                                        nccTGGCTGCTCGAACGCCCACTGCGCGTCGACCTGGCGCTAATTCGCGCTCATCGTTGCGACACACTTGGCAACCTGACCTATCAACTTAGCGCCCGCAACTTTAACCCCCTGATAGCCCTTGCGGCTGATATCACGCTGGTAGAGCCAGATGAACTGGTCGAAACCGGCGAGCTGCAACCTGACCATATTGTCACCCCTGGTGCCGTTATCGACCACATCATCGTTTCACAGgag  >  1:94951/2‑237 (MQ=255)                                                                                                                                                                                         ccTGGCTGCTAGAACGCCCACTGCGCGTTGACCTGGCGCTAATTCGCGCTCATCGTTGCGACACACTTGGCAACCTGACCTATCAACTTAGCGCCCGCAACTTTAACCCCCTGATAGCCCTTGCGGCTGATATNACGNNNNNagnnnnnnnnnn                                                                                    >  2:193308/1‑144 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                 GGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGTACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACGCCCACTGCGCGCCGACCTGGCGCTAATTCGCGCTCATCGTTGCGACACACTTGGCAACCTGACCTATCAACTTAGCGCCCGCAACTTTAACCCCCTGATAGCCCTTGCGGCTGATATCACGCTGGTAGAGCCAGATGAACTGGTCGAAACCGGCGAGCTGCAACCTGACCATATTGTCACCCCTGGTGCCGTTATCGACCACATCATCGTTTCACAGGAG  >  NC_000913/2323681‑2324100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑