Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,320,013	G→A	R217R (CGC→CGT)	phnI ←	carbon‑phosphorus lyase core complex subunit PhnI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,320,013	0	G	A	94.7%	49.8 / NA	19	R217R (CGC→CGT)	phnI	carbon‑phosphorus lyase core complex subunit PhnI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (8/10);  minor base C (1/0);  total (9/10)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGT  >  NC_000913/4319776‑4320234                                                                                                                                                                                                                                              |                                                                                                                                                                                                                              ggCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTACGCCCGTAACcgc                                                                                                                                                                                                                  <  1:160550/251‑1 (MQ=255)                                  catcgcTTTGCGCTCGCTCATGCCGAACGCCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCCCCCGGCAGGTCGATAAAACCGTTAACCATCTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCGTGCGAACGGGTGATTACGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGc                                                                                                                                                                                 <  1:146244/251‑1 (MQ=255)                                              ctcgctcATGCCGAACACCAGCCCGTAGCCGCGCGTGCAGTGCGGCGGCTCACCCGGCGGGTCGATAGAACCGTTAACCATTTCACACTCAGTCATCAGCAGTCCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGGGATTACGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTagccgc                                                                                                                                                                     >  1:211474/1‑251 (MQ=255)                                                       gCCGAACACCAGCCCGTAGCCGCGCGGGAAGTGCGGCGGCGCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTGCCGGCACAATCGACACGGCGATATAGCCGCTGCGGATCTCGCCTGCGATCGGGTGATTACGCCCGTAGCCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAAc                                                                                                                                                             <  1:181086/250‑1 (MQ=255)                                                               ccAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTACGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCaa                                                                                                                                                     >  1:18174/1‑250 (MQ=255)                                                                            gcgcgTGAATTGCGGCGGCTCACCCGGCGGGTCGATAACACCGTTAACCATTTCAAACTCAGTCATTCGCCCTTCGCCGACGTTCACAGCACATCCCAGGTCTTCCCGCCCAATCGACACGTCGATATACACGCTCCGGATCTCCCCTGCGAACGGGTGATTCCGCCCGTCACCGCCTTGGGTGGAGTAGGCCAGCGCCCGCAAATAGACTTCCTCGCCGCGCATCAAcctatcgtcacagcccacgtctt                                                                                                                                       >  1:220744/1‑229 (MQ=255)                                                                                    cgTGCGGCGGCTCACCCGGCGTGTCCATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCTACGGTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATATCCGCTGCGGATCTCGCCTGCGAACGGGTGATTACGCCCGTAACCGCGTTGGGTTGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCTCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAgggg                                                                                                                               <  1:330513/250‑1 (MQ=255)                                                                                    aGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTACGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCTCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAgggg                                                                                                                               <  1:322066/251‑1 (MQ=255)                                                                                     gTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTCCCGGCACAATCGACACGTCGATAGAGCCGCTGCGGATCTCGCCTTCGAACGGGTGATTACGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGt                                                                                                                              <  1:169375/251‑1 (MQ=255)                                                                                              cTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCATTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTACGCCCGTAACCGCGGTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCTCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAg                                                                                                                                  <  1:287460/238‑1 (MQ=255)                                                                                                         ggTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTACGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACgg                                                                                                                                                 <  1:73052/212‑1 (MQ=255)                                                                                                          gTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTACGACCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCcgcg                                                                                                                                                                    >  1:18679/1‑192 (MQ=255)                                                                                                                                tCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGACGATATAGCCCCTGAGGCTATCGCCTGCGAACGGGTGATTACGCCCGTAACCGCGTTGGGTGGAGTGGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGAGGCGTGCGGGTGATGt                                                                                                      >  1:327309/1‑232 (MQ=255)                                                                                                                                       cAGTCATCAGCAGTTCTCCGACGTTCACCGCAAATCCCAGCGCTTCCGGCACAATCGACACGTCGATATAGCCGCTGGGGATCTCGCCTGCGAACGGGTGATTACGCCCGTAACCGCGTTGTGTTGAGGAGGCCAGCGCCAGCAAATAGCCTGCGTCTCCGCGCATCGACTGCTGCAAACGTGAGGAGCGTTAGCATTGGTAAACCGGCGGGGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCctc                                                                             <  1:166329/250‑1 (MQ=255)                                                                                                                                                                  ccGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTACGCCCGTAACCGCGTTGGGTGGAGTAGgc                                                                                                                                                                                                  >  1:14853/1‑106 (MQ=255)                                                                                                                                                                   cGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTACGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTtcgtc                                                                                                                                                                          >  1:97788/1‑129 (MQ=255)                                                                                                                                                                            cAGCTCNNCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTACGCCCGTAACCGCGTTGGGTGGAGTAGgc                                                                                                                                                                                                  <  2:14853/96‑1 (MQ=255)                                                                                                                                                                                          caATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTACGCCCGTAACCGAGTTGGGTGGCGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCAGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGAc                         >  1:208647/1‑251 (MQ=255)                                                                                                                                                                                                                 cTGCGGATCTCGCCTGCGAACGGGTGATTACGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTACACCGGCGGCGTGCGGGTGCTGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTcggcggg  >  1:172677/1‑250 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                              GGCTTGCAGAGCGCGGTCGACCAGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGT  >  NC_000913/4319776‑4320234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑