Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	828,572	C→T	G137D (GGC→GAC)	ybhF ←	ABC exporter ATP binding subunit YbhF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	828,572	0	C	T	100.0%	40.3 / NA	15	G137D (GGC→GAC)	ybhF	ABC exporter ATP binding subunit YbhF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/8);  total (7/8)

CACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATC  >  NC_000913/828366‑828784                                                                                                                                                                                                               |                                                                                                                                                                                                                     cACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGCGAGTTAACATCCCTTCGCCCGCCAGCTCATGCACTATCTGCCCGAGTTCGCGCCGTGAGATAGGGTACACGCCGCCGCCGGGTTCATCGAGAAGCAACACTCTCGCTACGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGTCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCcagc                                                                                                                                                                          >  1:172261/1‑251 (MQ=255)                           tACGAGGTACTCCAGAGGTTTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACTCCGACGCCTGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGTCCGCCAGGCGTCCGGTAAAc                                                                                                                                                                                                  <  1:121727/201‑1 (MQ=255)                                                                      cATGCACCATCGGCAACAGTCCGCGCCGGGAGATAGGGTCAACGCCGACGCCGGGTTCAGCGAGCAGCAACACTTTCGGTTCGCCCAACAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGTCCGCCAGGCGGCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTGACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGt                                                                                                       <  1:2903/249‑1 (MQ=255)                                                                                  gCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGTCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCAc                                                                                             <  1:89929/247‑1 (MQ=255)                                                                                               ncGTGAGATAGGGTCAACGCCGACGCCGGGTTCACCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCACAACGAGTTTTTGTTTCATCCCACCGGAGAGCTTGTCCGCCAGGCGTcc                                                                                                                                                                                                         >  1:60094/2‑126 (MQ=255)                                                                                                   gagaTAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGTCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGAcn                                                                        <  1:79973/251‑2 (MQ=255)                                                                                                         gggTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGTCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAAttt                                                                  >  1:5970/1‑251 (MQ=255)                                                                                                            tCAACGCCGACGCCGGGGTCATCGAGCAGCAACCCTTTCGGTCCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCCCCGGAGAGCTTGTCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGAc                                                                                                                                    <  1:14116/182‑1 (MQ=255)                                                                                                                                                ttCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGTCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGc                                                           <  1:248747/219‑1 (MQ=255)                                                                                                                                                         cccACNAGGGTNCNNTCCAGACCGAGTTTTTGTTNCATCCCACCGGAGAGNTTGTNCGCCANGCNt                                                                                                                                                                                                           >  2:120844/1‑66 (MQ=255)                                                                                                                                                         cccACCAGGGTACAGGCCAGACCGAGTTTTGGTTTCATCCCACCGGAGAGCTGGTCCGCCAGGCGTCCGGTAAACGGCCCAagag                                                                                                                                                                                        <  1:120844/85‑1 (MQ=255)                                                                                                                                                                         ncAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGTCCGCCAGGCGTCCGGTAAACGACCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATc  >  1:79513/2‑251 (MQ=255)                                                                                                                                                                                        gTTTCATCNCNNNGNNGAGCTTGTCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAANCTCCAGCCGGCGAGCNAANGTttncttnc                                                                                                                                                    >  2:233376/1‑90 (MQ=255)                                                                                                                                                                                        gTTTCATCCCCCCTGAGAGCTTGTCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTAc                                                                                                                                                    <  1:233376/90‑1 (MQ=255)                                                                                                                                                                                                          gCTTGTCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGAc                                                                         >  1:188927/1‑147 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                     CACGGCACTGCTCGGCTTCGTCGAGATACGAGGTACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTACAGATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATC  >  NC_000913/828366‑828784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑