Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,444,895 C→T R112H (CGC→CAC)  rpsE ← 30S ribosomal subunit protein S5

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,444,8950CT100.0% 61.3 / NA 22R112H (CGC→CAC) rpsE30S ribosomal subunit protein S5
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/13);  total (9/13)

GCAGACGACCGATTGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGC  >  NC_000913/3444671‑3445111
                                                                                                                                                                                                                                |                                                                                                                                                                                                                        
gCAGACGACCGATTGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCACCGGGTTTACCACGCTTGGCAGCGACCATTTCTGGAGACTTCATATTTTCCAGGCCATCAATAGTTGCCCGAACCACGTTGCTCGGGATGGTGGAACCATAGGCTTTAGCCAGACCGTTATGAACCCCAGCGACTTCAAGAACACCGTGCATTCCACCACCGGCGCTGATACCg                                                                                                                                                                                                >  1:234811/1‑251 (MQ=255)
       acCGATTGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCATTGCACCACCGGCGATGATACCGGTACCtt                                                                                                                                                                                         >  1:26301/1‑251 (MQ=255)
                             gTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAGCGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATAc                                                                                                                                                                   <  1:309625/251‑1 (MQ=255)
                                      aTAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAAcc                                                                                                                                                          <  1:1248/251‑1 (MQ=255)
                                           cTTTGCCATGGTTTATTTCCACAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCATTGcac                                                                                                                                                                                                                 >  1:68392/1‑191 (MQ=255)
                                           cNTTGNCNNGNNTTANTTCCCCAGAATTTCTTCAACGNNTTTACCACGNTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCNCGAACCACGNNGANCGGGGNGNNNGNNNCANAGGCTTTAGCCAGAACGTTNTGNNCCCCAGNGACTTCCAGAACGGCGTGCATTGcac                                                                                                                                                                                                                 <  2:68392/191‑1 (MQ=255)
                                                ccATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATAcgc                                                                                                                                                                 <  1:1886/234‑1 (MQ=255)
                                                ccATGGTTTATTGCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCATTg                                                                                                                                                                                                                    <  1:147575/183‑1 (MQ=255)
                                                     gTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTGATGAACCCCAGCGACTTCCAGAACGGCGTGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATAcgcg                                                                                                                                                                <  1:226385/230‑1 (MQ=255)
                                                                  gAATTTCTTCAACGGATTTACCACGCGTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCAGTGCACCACCGGCGATGATACCGGTACCTTCGGAATCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGc                                                                                                                              <  1:311339/251‑1 (MQ=255)
                                                                        cttcAACGGATTTACCACGCTTGGCAGCGACCATTTCTGCAGAATTCATCTTTTCCAGCCCATCACTAGTTGCCCGAACCACGCTGAGCGGGTTGGTGGAACCATCGGCTTTAGCCAGTACGTTATTAACCCCAGCGACTTCAAGCACGTCGTGCATTGCCCCACCGGCGCTGATACCGGTACCTTCGGCAGCCGGCTGCCTGAATCCGCGTGAAc                                                                                                                                                           >  1:239289/1‑216 (MQ=255)
                                                                                                    gACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCatat                                                                                            >  1:16376/1‑251 (MQ=255)
                                                                                                                                  gCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGc                                                                                                                              >  1:288226/1‑187 (MQ=255)
                                                                                                                                      tCAAGAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAGCGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCATTGCACCACCGGCGATGATACCGGTACCGTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAggg                                                                                                                                    <  1:53999/177‑1 (MQ=255)
                                                                                                                                                cACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCATTGCNNCACCgg                                                                                                                                                                                                           >  2:5073/1‑96 (MQ=255)
                                                                                                                                                cACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGcc                                                                                                                      <  1:5073/181‑1 (MQ=255)
                                                                                                                                                                      gnnggAACCNNAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGc                                                                                                                                                                                  <  2:26301/99‑1 (MQ=255)
                                                                                                                                                                                              aaCGTTATGAACCCCAGCGACTTCCAGAACGGCGTGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGc  <  1:105455/251‑1 (MQ=255)
                                                                                                                                                                                                        aCCCCAGCGACTTCCAGAACGGCGTGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCgtgtn                                                                                                                                                    <  1:81277/95‑2 (MQ=255)
                                                                                                                                                                                                          cccAGNGACTTNCNNAACGGCGTGCCTTGCACCANCGGCGATGATACCGGNACCTNCGGAANCCngn                                                                                                                                                                              >  2:134020/1‑66 (MQ=255)
                                                                                                                                                                                                                gACTTCCAGAACGGCGTTCATTGCACCACCGGCGATGATACCGGTGCCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCag                                                                                                                            <  1:280341/111‑1 (MQ=255)
                                                                                                                                                                                                                            ggCGTGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTTCGACGGGCTTTTTTCATCGc                                                                      >  1:21744/1‑153 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                                        
GCAGACGACCGATTGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGC  >  NC_000913/3444671‑3445111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑