Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	794,007	C→T	G213D (GGC→GAC)	modE ←	DNA‑binding transcriptional dual regulator ModE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	794,007	0	C	T	100.0%	25.7 / NA	11	G213D (GGC→GAC)	modE	DNA‑binding transcriptional dual regulator ModE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/5);  total (6/5)

GCAAAATTTGCAACGATGACATTTTATATCCCACTTTGTGCAGCGATTACTGACAGGGATACGTGTTTCATAACAAATTGTCAACACGCTTAGCACAGCGTGGCGATAATCACGCTGTCGGCATTAAAGTAGGCCGTGACATTCTGTCCTTGCTGAAGAGAAGTCGCTTCATTTACCGGCACTGTGGCGCACAGTGTTTGCCCGTCGGGTAGCGCCATTAATACTTCGCACTGCTCTGCGCCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGG  >  NC_000913/793767‑794244                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              gCAAAATTTGCAACGATGACATTTTATATCCCACTTTGTGCAGCGATTACTGACAGGGATACGTGTTTCATAACAAATTGTCAACACGCTTAGCACAGCGTGGCGATAATCACGCTGTCGGCATTAAAGTAGGCCGTGACATTCTGTCCTTGCTGAAGAGAAGTCGCTTCATTTACCGGCACTGTGGCGCACAGTGTTTGCCCGTCGGGTAGCGCCATTAATACTTCGCACTGCTCTGCGTCGCGCTCAat                                                                                                                                                                                                                                     >  1:72570/1‑251 (MQ=255)      aTTTGCAACGATGACATTTTATATCCCACTTTGTGCAGCGATTACTGAACGGGATACGAGTTTCATAACAAATTGTAAACACGCTAAGCACAGCGTGGAGCTAATCACGCTGTCGGCATTAAAGTAGGCCGTGACATTCTGTCCTTGCTGAAGAGAAGTCGCTTCATTTACCGGCAATGTGGCGCACAGCGTTTGCCCGTCGGGTCGCGCCATTAATACTTCGCACTGCTCTGCGTCGCGCTCAATATGAc                                                                                                                                                                                                                                >  1:158997/1‑251 (MQ=255)                aTGACATTTTATATCCCACTTTGTGCAGCGATTACTGACAGGGATACGCGTTTCATAACAAATTGTCAACACGCTTAGCACAGCGTGGCGATAATCACGCTGTCGGCATTAAAGTAGGCCGTGACATTCTGTCCTTGCTGAAGAGAAGTCGCTTCATTTACCGGCACTGTGGCGCACAGTGTTTGCCCGTCGGGTAGCGCCATTAATACTTCGCACTGCTCTGCGTCGCGCTCAATATGACTAATAATAcc                                                                                                                                                                                                                      >  1:277579/1‑251 (MQ=255)                                  cNTTGTGAAGAGATTACTGACAGGGATCCGTGTTTCCTAACAAATTGTCAACACGCTTAGCACAGCGTGGCGATAATCACGCTGTCGGCATTAAAGTAGGCCGTGACATTCTGTCCTTGCTGAAGAGAAGTCGCTTCATTTACCGGCACTGTGGCGCACAGTGTTTGCCCGTCGGGGAGCGCCATTAATACTTCGCACTGCTCTGcgtcgc                                                                                                                                                                                                                                            >  1:254912/1‑211 (MQ=255)                                                                        aaCAAATTGTCAACACGCTTAGCACAGCGTGGCGATAATCACGCTGTCGGCATTAAAGTAGGCCGTGACATTCTGTCCTTGCTGAAGAGAAGTCGCTTCATTTACCGGCACTGTGGCGCACAGTGTTTGCCTGTCGGGTAGCGCCATTAATACTTCGCACTGCTCTGCGTCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAAtacctaccn                                                                                                                                                              <  1:95343/251‑2 (MQ=255)                                                                                                       gCGATAATCACGCTGTCGGCATTAAAGTAGGCCGTGACATTCTGTCCTTGCTGAAGAGAAGTCGCTTCATTTACCGGCCCTGTGGCGCACAGTGTTTGCCCGTCGGGTAGCGCCATTAATACTTCGCACTGCTCTGCGTCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCg                                                                                                                                                                                      >  1:305550/1‑196 (MQ=255)                                                                                                                 cGCTGTCGGCATTAAAGTAGGCCGTGACATTCTGTCCTTGCTGAAGAGAAGTCGCTTCATTTACCGGCACTGTGGCGCACAGTGTTTGCCCGTCGGGTAGCGCCATTAATACTTCGCACGGCTCTGCGTCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATcn                                                                                                                     <  1:57875/251‑2 (MQ=255)                                                                                                                              aaaGTAGGCCGTGACATTCTGTCCTTGCTGAAGAGAAGTCGCTTCATTTACCGGCACTGTGGCGCACAGTGTCTGCCCGTCGGGTAGCGCCATTAATACTTCGCACTGCCCTGCGTCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTAGTACTGCGTAATACCTACCCCCGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATcc                                                                                                                     >  1:179178/1‑238 (MQ=255)                                                                                                                                aGTAGGCCTTGACATTCTGTCCTTGCTGAAGAGAATTCGCTTCATTTACCGGCACTGTGGCGCACAGTTTTTGCCCGTCGGGTAGCGCCATTAATCCTTCGCACTGCTCTGCGTCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGAcgctcn                                                                                                      <  1:194619/251‑4 (MQ=255)                                                                                                                                             aTTCTGTACTAGCTGAAGAGAAGTCGCTTCATCTACCGGAACTGTGGCGCCCAGAGTTTGCCCGTCGGGTAGAGCCCTTAATACTTCGCACTGCTCTGCGTCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATc                                                                                                                                          >  1:117735/1‑202 (MQ=255)                                                                                                                                                                               nccNGCANTNNGNNGCANAGTGTTTGCCCGTCGGGTTGCNNCATGAATACNTCGCACTGCTCTGCGTCGCGCTCAATATGACAAAAAATACCCGGTAATTGGTTNTCAGCGTTTNNCGNTTCCGNCNNNTNNNGANTAAGACCTACCCGCGGCGCNTTNNGCAATANCAACACTTCTTTGCCTTCATCCAGcc                                                                                                                 <  2:72570/192‑1 (MQ=255)                                                                                                                                                                                                                                    gCACTGCTCTGCGTCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCTACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATgg  <  1:114307/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              GCAAAATTTGCAACGATGACATTTTATATCCCACTTTGTGCAGCGATTACTGACAGGGATACGTGTTTCATAACAAATTGTCAACACGCTTAGCACAGCGTGGCGATAATCACGCTGTCGGCATTAAAGTAGGCCGTGACATTCTGTCCTTGCTGAAGAGAAGTCGCTTCATTTACCGGCACTGTGGCGCACAGTGTTTGCCCGTCGGGTAGCGCCATTAATACTTCGCACTGCTCTGCGCCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGG  >  NC_000913/793767‑794244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑