Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,213,597	G→A	A122T (GCA→ACA)	yjaA →	stress response protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,213,597	0	G	A	100.0%	38.8 / NA	16	A122T (GCA→ACA)	yjaA	stress response protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/8);  total (8/8)

TCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTGGGGCAACGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGG‑TGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTG  >  NC_000913/4213371‑4213828                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                         tCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTGGGGCAACGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGACAATGCTTAAGCAGAAATAATCgt                                                                                                                                                                                                                  >  1:5401/1‑251 (MQ=255)    gCAAGATCTTGTTCTGCAACTCCCCCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGTTTGAGGAACGCATTCGCCATGAAGTGGTCGCTGGGGCAACGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCGGCGGTACTCAGTGATAGTGCGGTCATGACAATGCTTAAGCAGAAATAATCgtn                                                                                                                                                                                                                 <  1:34575/249‑2 (MQ=255)           cTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTGGGGCAACGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGACAATGCTTAAGCAGAAATAATCGTGTCACCATtg                                                                                                                                                                                                        >  1:289443/1‑251 (MQ=255)                   gCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTGGGGCAACGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGACAATGCTTAAGCAGAAATAATCGTGTCACCATTGG‑Tggg                                                                                                                                                                                                  <  1:17550/248‑1 (MQ=255)                               acGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGGATGGATATGGTTGTGAGCTCACTGGAAATGAATGAGGGCTGTTTGTCACAGGTTGAGGAACGCCTTCTCCATGAAGTGGTCGCTGGGGCAACGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGACAATGCTTAAGCAGAAATAATCGTGTCACCATTGG‑TGGGTACTAAACCTGAANt                                                                                                                                                                                   <  1:254156/251‑1 (MQ=255)                                            cATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTGGGGCAACGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGACAATGCTTAAGCAGAAATAATCGTGTCTCCATTGG‑TGGGTACTAAACCTGAAGtt                                                                                                                                                                                  <  1:313697/239‑1 (MQ=255)                                                             aaaaCGTATGGATATTGTTGTGAGCGCACTGGAAATGACTGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTGGGGCAACGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGACAATGCTTAAGCAGAAATAATCGTGTCACCATTGG‑TGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCt                                                                                                                                                     >  1:111820/1‑251 (MQ=255)                                                                                                         gCGGTTTGTCACATGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTGTGGCAACGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGACAATGCGTAAGCAGAAATAATCGTGTCACCATTGG‑TGGGTACTAAACCTTAAGTTCAGCCCAGCGGGATGAGAAAAAATCGCCTACCCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcccc                                                                                                         <  1:139713/251‑1 (MQ=255)                                                                                                                                        ccATGAAGTGGTCGCTGGGGCAACGTTCATGCAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGACAATGCTTAAGCAGAAATAATCGTGTCACCATTGG‑TGGGTACTAAAc                                                                                                                                                                                          >  1:277874/1‑139 (MQ=255)                                                                                                                                               ntGGTCGCTGGGGCAACGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGACAATGCTTAAGCAGAAATAATCGTGTCACCATTGG‑TGGGTACTACACCTGAAGTTCAGCCCACCgg                                                                                                                                                                       >  1:192379/2‑151 (MQ=255)                                                                                                                                                 ngTCGCTGGGGCAACGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGACAATGCTTAAGCAGAAATAATCGTGTCACCATTGG‑TGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAccc                                                                  >  1:202840/2‑250 (MQ=255)                                                                                                                                                        nnnnnaaCNTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTAAGAAGGGAGAGGGAGGGAAGGAAAAGGATTAAGCGGAAATAATCGTGTCACCATTGG‑TGGGTACTAAACCTGAAGTTCAGCCCACCgg                                                                                                                                                                       <  2:192379/137‑1 (MQ=255)                                                                                                                                                         gggCAACGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGACAATGCTTCAGCAGAAATAATCGTGTCACCATTGG‑TGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGATAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGCTACGGTTTCCCCACATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACcttctt                                                          >  1:329334/1‑250 (MQ=255)                                                                                                                                                               cGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGACAATGCTTAAGCAGAAATAATCGTGTCACCATTGG‑GGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGc                                                                                                                                                 >  1:6746/1‑157 (MQ=255)                                                                                                                                                                       aaaTATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGACAATGCTTAAGCAGAAATAATCGTGTCACCATTGG‑TGGNt                                                                                                                                                                                                 <  1:253851/101‑1 (MQ=255)                                                                                                                                                                                                                cAGTGAGAGTGCGGTCATGACAATGCTTAAGCAAAAAGAATCTTGTC‑CCATTGGGTGGGTACTAACCCTGAAGTGCAGCCCACGGGGATGAAAAAAAACCGCCGCCCCCCCCAGATACGCCACATGCAGAAGCGGAGACGTTTTCCCAAAATCTTCCGCCCCAGAGGGCCCCGGACCCTAAAATCCCTCCTTCAAAAAAAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTg  <  1:158474/251‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                         TCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTGGGGCAACGTTAATGAAATATCGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGG‑TGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGGTTGTCAGTTCCGGTG  >  NC_000913/4213371‑4213828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑