Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,893,087	C→T	G50D (GGC→GAC)	yoaA ←	putative ATP‑dependent 5' to 3' DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,893,087	0	C	T	100.0%	40.0 / NA	16	G50D (GGC→GAC)	yoaA	putative ATP‑dependent 5' to 3' DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/10);  total (6/10)

ATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATCGTCCGTCACACTACCCCCTGTTGATTTGAACAGGGATTATGTCAGGATGAGGGCGCTTTCGCCAGTTGAAGTGGTGACGGCGACCTCACATTGTGGCAGT  >  NC_000913/1892849‑1893326                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                aTCGCTTAAGATTTGTACCGGCAGATCGCCCCCCGCCCGCGCCTGCTGTTCGAGACGGTCGAGGCGGAGGAAATTTGAGAACCCTTTCAGCAGCTCCACGTTGCCCGTATATTTCCATGCCTTTCAGACTGTTGGCCAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTAAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGTCCGTACCGGTTc                                                                                                                                                                                                                                     >  1:160375/1‑251 (MQ=255)                             ccccccGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGCGCGCGCTTTCACCATCACAACGTTGCCAGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCCGCTCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCACCCGCAGCGCAGGCGCCAGGTAAGCGTAGGTTTTGTCCGTACCGGTTCCTGCt                                                                                                                                                                                                                                >  1:145853/1‑228 (MQ=255)                                         gcCTGCTGGTCGAGACGTTCGAGGCAGATGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGTCCTTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCta                                                                                                                                                                                             <  1:140804/251‑2 (MQ=255)                                                                     ggTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGTCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCtgn                                                                                                                                                                 <  1:95147/251‑2 (MQ=255)                                                                              aGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGTCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTcgn                                                                                                                                                        <  1:66234/251‑2 (MQ=255)                                                                                  cccTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGTCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTggg                                                                                                                                                                                           <  1:99805/212‑1 (MQ=255)                                                                                                ccACGTTGTCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGTCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTGCTCGCGGCTTAAAGCCTGGTATc                                                                                                                                      <  1:259836/251‑1 (MQ=255)                                                                                                                         cTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGTCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGGGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTAGTg                                                                                                             >  1:17594/1‑251 (MQ=255)                                                                                                                           ttGAGACTGTTGGCAAATCGCGGCTGTAGAGCGGATCCTGCAACGCTTTTGAGTCGGTCGAGATAATGATTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTATGTTTTGTCCGTGCCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTGTTCTATCTCCTGGGTGACGGCTACCGCCTTCGTTCGCTGTGGTTCTCGCGGCGTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTg                                                                                                             <  1:184087/249‑1 (MQ=255)                                                                                                                                                            gATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTGTTCGCCCGCAGCGCAGGAGCCATGTAAGCGTAGGTTTTGTCCGTACCGGTTCCTGCTTCCCCCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGTTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATCGTCCGTCACACTACCCCCTGTTGAtt                                                                            <  1:26762/249‑1 (MQ=255)                                                                                                                                                                  gCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGAGCAGGAGCCAGGTAAGCGTAGGTTTTGTCCGTACCGGTTCCTGCTTCCACCACCCGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCCTCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATCGTCCGTCACACTACCCCCTGTTGATTTGAACAgg                                                                    >  1:219859/1‑251 (MQ=255)                                                                                                                                                                             gAGCCGGTCGAGATAATGAATTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGAAGGTTTTGTCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGGGCAAAATCGTCCGTCACACTACCCCCTGTTGATTTGAACAGGGATTATGTc                                                           >  1:92963/1‑249 (MQ=255)                                                                                                                                                                                       agaTAATGTCTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTGTTGTCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACTGCTACCGCCATCTGTCGCTGTGGGGCTCTCGGCTTAAAGCCTGGTATCTCTTTCGCCAGCTGACCGTCTGGTTCAAAATCGTCCGTcac                                                                                             <  1:264273/205‑1 (MQ=255)                                                                                                                                                                                                              cgcagcgcagGAGCCAGGTAAGCGTAGGTTTTGTCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTGCCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATCGTCCGTCACACTACCCCCTGTTGATTTGAACAGGGATTATGTCAGGATGAGGGCGCTTTCGCCAGTTGAAGTGGTGan                        <  1:198529/251‑2 (MQ=255)                                                                                                                                                                                                                  gcgcagGAGCCAGGTAAGCGTAGGTTTTGTCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATCGTCCTGCACACTACCCCCTGTTGATTTGAACAGTGATTATGTCAGGATGAGGGCGCTTTCGCCAGTTGAAgtggtg                          <  1:49247/245‑1 (MQ=255)                                                                                                                                                                                                                                    cGTAGGTTTTGTCCATACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATCGTCCGTCACACTACCCCCTGTTGATTTGAACAGGGATTATGTCAGGATGAGGGCGCTTTCGCCAGTTGAAGTGGTGACGGCGACCTCACTTTGTGGCAgg  >  1:89362/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                ATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATCGTCCGTCACACTACCCCCTGTTGATTTGAACAGGGATTATGTCAGGATGAGGGCGCTTTCGCCAGTTGAAGTGGTGACGGCGACCTCACATTGTGGCAGT  >  NC_000913/1892849‑1893326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑