Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	294,754	C→T	G16R (GGA→AGA)	yagM ←	protein YagM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	294,754	0	C	T	100.0%	31.6 / NA	14	G16R (GGA→AGA)	yagM	protein YagM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/7);  total (7/7)

GCATTTTTCTAATATAGGGATAGCCAAATCCACAACTTCCGCTATTACGGAAGAGTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTGTTTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGT  >  NC_000913/294560‑294911                                                                                                                                                                                                   |                                                                                                                                                              ncATTTTTCTAATATAGGGATAGCCAAATCCACAACTTCCGCTATTACGGAAGAGTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTGTTTTTGCCTTGACAAGGTTTTTTTCTTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGaa                                                                                                        >  1:30301/2‑250 (MQ=255)  cATTTTTCTAATATAGGTATAGCCAAATCCATAACGTCCGCTAGTACGGAAGAGTGAGATTTATTTGGTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATGGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTGTTCTTGCCTTGGCAAGGTTTTTTGCTTGGTGGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAAc                                                                                                      <  1:218718/251‑1 (MQ=255)         cTAATATAGGGATAGCCAAATCCACAACTTCCGCTATTACGGAAGAGTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTGTTTTTGCCTTGACAAGGTTTTTGTCTTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACAttt                                                                                                                <  1:284983/234‑1 (MQ=255)                aGGGATAGCCAAATCCACAACTTCCGNTATTACNNAANAGTGAGATTTATTTGTTAGNCTNNNANNNTNATCAANTANNCCTAATGAANATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCNGTGACTCATNNTTTTTGCCTTGACAAGGTTTTNTTCNNGNNANAACnc                                                                                                                                                    >  2:76581/1‑191 (MQ=255)                aGGGATAGCCAAATCCACAACTTCCGCTATTACGGAAGAGTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTGTTTTTGCCTTGACAAGGTTTTTTTCTTGGTAGAACgc                                                                                                                                                    <  1:76581/191‑1 (MQ=255)                aGGGATAGCCAAATCCACAACTTCCGCTATTACGGAAGAGTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTGTTTTTGCCTTGACAAGGTTTTTTTCTTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACAGTTTTAAGGAAACCTCTTTGTTAtctc                                                                                        <  1:248656/251‑1 (MQ=255)                       gCCAAATCCACAACTTCCGCTATTACGGAAGAGTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGCGTAGTCTGTACTACCTGTGACTCATTGTTTTTGCCTTGACAAGGTTTTTTTCTTGGTAGAACGCTGCTCATCTCAAAAtt                                                                                                                                    >  1:219007/1‑200 (MQ=255)                                                                                 aaTGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGCCTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTGTTTTTGCCTTGACAAGGTTTTTTTCTTGGTAGCACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAg                                                                                                           >  1:133577/1‑167 (MQ=255)                                                                                  aTGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGCAGTAGTCTGTAATACCTGTGACTCATTGTTTTTGCCTTGACACGGTTTTTTTCTTGGTAGAACGCTGATCATCTCAAAATTAGTACAACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCACTATAAAACTACTATCTGAAATTTCTCAGTGTTGCCACAtt                                              >  1:287173/1‑227 (MQ=255)                                                                                                cTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTGTTTTTGCCTTGACAAGGTTTTTTTCTTGGTAGAACGCTGCTCAtc                                                                                                                                            >  1:191268/1‑119 (MQ=255)                                                                                                      aaGATTGATTTGTGAACGTGACTGAGTGTTGTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTGTTTTTGCCTTGACAAGGTTTTGTTCTTGGTAGAACGCTGCTCATCGCAAAATTAGTAATGCTATTAGACATTTTTAAGGAAACCTCTTTGGTATCTCAATATAAAACTACTATCTGAAATTTCTCAGGGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGt  <  1:321449/251‑1 (MQ=255)                                                                                                                          aCTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATGGTTTTTGCCTTGACAAGGTTTTTTTCTTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTAGTAGACATTTTTAAGGAAGCCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTa         <  1:157997/224‑1 (MQ=255)                                                                                                                                               gtagtCTGTACTACCTGTGACTCATTGTTTTTGCCTTGACAAGGTTTTTTTCTTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGcccc                                                 >  1:21054/1‑163 (MQ=255)                                                                                                                                                                                  ttGACAAGGTTTTTTTCTTGGTAGAACGCTGCTCATCTCAAAATTAGTACCAATATTCGACATTTTTAAGGAAAACTCTTTGTTATCTCAATATAAAAATAATATCTGAAATTTCTAAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTa         >  1:161483/1‑168 (MQ=255)                                                                                                                                                                                      cNAGNTTTTTNTCTTNGTAGAACGCTGCTCANCTCAAAATGAGTAACACTANNANACATGNTTAAg                                                                                                           <  2:133577/66‑1 (MQ=255)                                                                                                                                                                                                   |                                                                                                                                                              GCATTTTTCTAATATAGGGATAGCCAAATCCACAACTTCCGCTATTACGGAAGAGTGAGATTTATTTGTTAGTCTGGCATAATGATCAATTACTCCTAATGAAGATTGATTTTTGAACGTGACTGAGTGTTTTTTTATGGGAGTAGTCTGTACTACCTGTGACTCATTGTTTTTGCCTTGACAAGGTTTTTTTCCTGGTAGAACGCTGCTCATCTCAAAATTAGTAACACTATTAGACATTTTTAAGGAAACCTCTTTGTTATCTCAATATAAAACTACTATCTGAAATTTCTCAGTGTTGCCCCATTTTTCTCGGGGCAACATCGCTATAAATCAATAAAATTAAATCTGT  >  NC_000913/294560‑294911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑