Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,810,624	C→T	I138I (ATC→ATT)	ydjM →	inner membrane protein YdjM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,810,624	0	C	T	100.0%	34.8 / NA	13	I138I (ATC→ATT)	ydjM	inner membrane protein YdjM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/5);  total (8/5)

TCAAAACCGATCGCCCGCGCTTTTGGGCATCGTGGTTTTACCCACAGTCTGCTGGCGGTATTTGCGCTGCTGGCAACCTTTTACCTTAAGGTTCCGGAAGGCTGGTTCATTCCGGCTGATGCGCTACAAGGAATGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTT  >  NC_000913/1810394‑1810870                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       tCAAAACCGATCGCCCGCGCTTTTGGGCATCGTGGTTTTCCCCACAGTCTGCTAGCGGTCTTTGCGCTGCTGGCAACCTTTTACATTAAGGTTCCGACAGGCTGGTTCATTACGGCTGATGCGCTACAAGGAATGGGGCTGGGTTCTTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAACAGGGca                                                                                                                                                                                                                                     >  1:184891/1‑250 (MQ=255)              cccGCGTTTTTGGGCATCGGGTTTTTACCCCCATTCTGCTGGCGTTATTTGCGCTGCTGGCAACCTTCTACCTTAAGTTCCCGGAATCCTGGTTCATTCCGGCTGATGCGCAACAAGAAATGGTGCTGGTTTAGTTGCGCCACATACTTGCCGATAGGCTGACACCCGCCGGTGTTCCCCTGCTCGGGCCATGCCGCTGTCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAAc                                                                                                                                                                                                                         <  1:121636/249‑1 (MQ=255)                                                                                                       tggttcagccgggCTGATGCGTTACAAGGAATGGTGGTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACCCCCGCCGGTGTTCCCCTGGTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAGCTGGAACGGTTTATCTGCATGGCATTATTTGTGTGGTCGGTAGGGATTCCCCATTCATTGCCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGa                                                                                                                               <  1:163427/239‑1 (MQ=255)                                                                                                        ggTTCATTCCGGCTGATGCGCTACAAGGAATGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCGGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAgcgc                                                                                                                                                      <  1:269078/226‑1 (MQ=255)                                                                                                            cATTCCGGCTGATGCGCTACAAGGAATGGAGCTGGGTCATTTGAGCCACATACTTGACGATAAGATGAAAACCGCCGGTGATCCCCTGCTCTGGGCATGCCGCTGGCGTTTCCGCTTGACTATTCTGGCTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTg                                                                                                                                                                                                               >  1:299579/1‑165 (MQ=255)                                                                                                                                                          cacaTACTTGCCGATATGCTGACACCCGCCGGTGTTCACCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAACAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTCTTTGTCTGGTCGGTATGGATg                                                                                                                                                                                >  1:312030/1‑150 (MQ=255)                                                                                                                                                                    acttgttctcttccgatcgcgGTGTTCCCCCGCTCTGGCCACGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTGCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCACATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGc                                                                 <  1:8721/232‑1 (MQ=255)                                                                                                                                                                           gCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCCACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGcc                                                                                                                                                                              >  1:112173/1‑135 (MQ=255)                                                                                                                                                                                       cgGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCNNCATGGCnnt                                                                                                                                                                                                      >  2:8721/1‑99 (MQ=255)                                                                                                                                                                                                                 gCTGGNGGNTNCGNNTNCCTANTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTANc                                                                                                                                                                                                                 >  2:148092/1‑62 (MQ=255)                                                                                                                                                                                                                           ccGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGCTCGGTATGGATGCCCCATTAATTACCCGAGACCAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACATTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTATGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTcac           >  1:166127/1‑250 (MQ=255)                                                                                                                                                                                                                                 gCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATTCCTTGCAGATCCATTTTCATCGGCTTATTAAGCATCATGTTGAATACTAAAAAGGCAGAAATCACCTTGCTGGAAGAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCtn    <  1:32283/251‑2 (MQ=255)                                                                                                                                                                                                                                   cTATTCTGGATCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGACATTATTTGTCTGGTCGGTATGGATGCCCAATTCCTTAACCGAGAAAAGCCCTGTTCGTTGGTCATCGCAAATGACAAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCCCCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGATCACAGTTCTGtt  >  1:284101/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       TCAAAACCGATCGCCCGCGCTTTTGGGCATCGTGGTTTTACCCACAGTCTGCTGGCGGTATTTGCGCTGCTGGCAACCTTTTACCTTAAGGTTCCGGAAGGCTGGTTCATTCCGGCTGATGCGCTACAAGGAATGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGTT  >  NC_000913/1810394‑1810870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑