Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,214,904	G→A	E209K (GAA→AAA)	metA →	homoserine O‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,214,904	0	G	A	100.0%	58.8 / NA	20	E209K (GAA→AAA)	metA	homoserine O‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/12);  total (8/12)

GCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGC  >  NC_000913/4214721‑4215144                                                                                                                                                                                        |                                                                                                                                                                                                                                                 gcgcTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTATCCGGCGTTTACGAGCATAATATTCTCCATCCTCATGCGCTTCTGAAGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGCTTCGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCtt                                                                                                                                                                               >  1:146420/1‑251 (MQ=255)                 aCGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTTGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATcc                                                                                                                                                                <  1:284219/250‑1 (MQ=255)                              gCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATTCGCTTCTGACGCGTGGCTTTGATGATGCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTGGCAGAGAc                                                                                                                                                                                                                                  <  1:217054/171‑1 (MQ=255)                                aaaCTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGATTCTGACGAGTGGATTTGATGATTCATTACTGGCACCGCATTCGCGCTATGGTGACTTTCCGGCAGCGTTGATTCGTGATTGCACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGAAGGGCCATc                                                                                                                                                                 >  1:326470/1‑234 (MQ=255)                                          cGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGcc                                                                                                                                                                                 >  1:184980/1‑208 (MQ=255)                                                     ctGGCGTTTACGAGCAGCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCGATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTGGCATAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCAGTTCCTTGTTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAtttt                                                                                                                             <  1:306626/249‑1 (MQ=255)                                                                   catcatATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTGCCGCGATGTGGAANc                                                                                                             <  1:253381/251‑1 (MQ=255)                                                                      catATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGTCAGCGTTGATTCGTGATTACGCCGATCTGAAAATTCTGGCATAGACGTAAGAAGGGGCTGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCTAATATGATGCGCAAACGCTGGCTCAGGAATTTTTCCGCGATGTGGAAGCCgt                                                                                                          <  1:313518/251‑2 (MQ=255)                                                                            ctcCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTc                                                                                                                           >  1:10504/1‑228 (MQ=255)                                                                                   cTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAttttt                                                                                                                            >  1:87838/1‑220 (MQ=255)                                                                                             tCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAgg                                                                                                                                   <  1:143773/203‑1 (MQ=255)                                                                                             tCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTAGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGtat                                                                                    >  1:122581/1‑250 (MQ=255)                                                                                                         ntttGATGATTCATTCCTGGCACCGCATTCGAGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCAAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGc                                                                       >  1:193009/2‑251 (MQ=255)                                                                                                                gatTCATTCCTGGCACCTCATTCGCGCTATGCTGACTTGCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGTATATCTTTTGGCCAGTAAAGATAAGCTCATTGCCTGTGTTACGGGCCATCCCGAATATGATgc                                                                                                                                                    <  1:107046/167‑1 (MQ=255)                                                                                                                             naCCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCa                                                          >  1:199438/2‑244 (MQ=255)                                                                                                                                   atgcgcgCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATAGGATGCGCAAACGCTTGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGtat                                                                                    <  1:288782/209‑1 (MQ=255)                                                                                                                                         gcTATGCTTACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGTTGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGCCGCAGGAATTTTTCCGCGAGGTGGAAGCCGGACTAGACCCGGAGGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCgagcgagc                                       <  1:243619/251‑1 (MQ=255)                                                                                                                                            aTGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGAc                                                                                                        <  1:184384/183‑1 (MQ=255)                                                                                                                                                           cAGCGTTGAATCGTGATTACACCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgtg                                                                                                                  <  1:316368/158‑1 (MQ=255)                                                                                                                                                                              cacCGATCTGAAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGTAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGc  <  1:170946/251‑1 (MQ=255)                                                                                                                                                                                        |                                                                                                                                                                                                                                                 GCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGC  >  NC_000913/4214721‑4215144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑