Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,344,613	G→A	intergenic (‑4/‑34)	yciZ ← / → ymiD	UPF0509 family protein YciZ/protein YmiD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,344,613	0	G	A	100.0%	41.4 / NA	15	intergenic (‑4/‑34)	yciZ/ymiD	UPF0509 family protein YciZ/protein YmiD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/6);  total (9/6)

TATAATACTTAATAAAAAAATACTGACGCGCCGGGCATCCTCAAATAGGAAAACCTGAAGGCACGATTGTTAAATCTCCCACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGCGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCAGAGTGGTAATCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTC  >  NC_000913/1344367‑1344853                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 tataATACTTAATAAAAAAATACTGACGCGCCGGGCATCCTCAAATAGGAAAACCTGAAGGCACGATTGTTAAATCTCCCACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGCGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCAGAGTGGTAATCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAAGAc                                                                                                                                                                                                                                               <  1:114729/250‑1 (MQ=255)                                          cAAATAGGAAAACCTGAAGGCACGATTGTTAAATCTCCCACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGCGCAAGAGTTCTGCTTTAAGTATCTCGAGATTAGGGATCGCAGAGTGGTAATCGCCTGCCACAAGTATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAAGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTg                                                                                                                                                                                                     <  1:270512/251‑1 (MQ=255)                                                            ggCACGATTGTTAAATCTCCCACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGCGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCAGCGTGGTAATCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCCTCAAATTCAGACATCAAAGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAgtgt                                                                                                                                                                                   >  1:135981/1‑251 (MQ=255)                                                                               ncACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTCGCGCAAGAGTTCTGCTTTAAGTATCTCGAGACTATGGATCGCAGAGTGGCCATCGCCTGCCACAAGGATATCTACCACACTATCAATACGTTCGGCACCGCGCTGGGCATCAAATTCAGACATCAAAGACATCCTTATAGCAAAAGGGGGACAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAAtt                                                                                                                                                                >  1:32456/2‑251 (MQ=255)                                                                                             ggATGTCTGGCGTAGATTCAGCAACCTGGCGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCAGAGTGGTAATCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAAGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAAt                                                                                                                                                   >  1:116971/1‑250 (MQ=255)                                                                                                                                                             gATCGCAGAGTGGTAATCGCCTGCCACAAGGATCTCTAACACAATATCAATACGTTCGGAAAAGCGCTGGGCATCAAATTCAGACATAAAAGACCTCCTTATCGCAAAAGGGGGCAAATGAATAATGCAAAAATTGCCGCTCAAAGCGAAGTGTTTAACAGCAACGGCTAATTATCATCAAGGAATACGATATATAATGACGGGATATAGCGCTAAGAATATATATTCATCTACTTATGCGCGCTTc                                                                                      >  1:246897/1‑247 (MQ=255)                                                                                                                                                                           aaTCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGAATCAAATTAAGACATCAAAGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGGGTTTAACAGCAACGGCTAATTATCATCCAGGCATACGATATATAATGCCGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATAcc                                                                      >  1:288320/1‑249 (MQ=255)                                                                                                                                                                            aTCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGTCATCAAAGACATTCTTATAGCAAAATGGGGAAAATGAATAAGGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCATCGGCGAATTATCATCCAGGAATACGATATATAATGACGGGATAGAGCGCTAAg                                                                                                                    <  1:208820/202‑1 (MQ=255)                                                                                                                                                                                         aGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAAGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTAGGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGan                                                      <  1:35979/251‑2 (MQ=255)                                                                                                                                                                                                                aCGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAAGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGGTTAACAGCAACGGCTAATTATCATCCACGAATACGATATATAATGACGGGATATAgcgc                                                                                                                        <  1:126504/162‑1 (MQ=255)                                                                                                                                                                                                                  gTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAAGACATCCTTATAGCAAAAGGGGGAACATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGAGTTTAACAGCAACGGCTAATTATAATCGAGGAATACGATATCTAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATCCCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGcaaacac                              >  1:146493/1‑249 (MQ=255)                                                                                                                                                                                                                      ggCAACGCGCTGGGCATCAAATTCAGACATCAAAGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTtat                                                                         >  1:268236/1‑203 (MQ=255)                                                                                                                                                                                                                                     aTCAAATTCAGACATCAAAGACATCCTTATAGCCAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAgc                                                                                                                          >  1:139085/1‑139 (MQ=255)                                                                                                                                                                                                                                        aaaTTCAGACATCAAAGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAACAGAGAAGTGTTTAAAAGCAACGGCTAATTATCATCCAGGAATACGat                                                                                                                                              >  1:209574/1‑116 (MQ=255)                                                                                                                                                                                                                                             cAGACATCAAAGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATGGATCCCGTTc  <  1:87829/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 TATAATACTTAATAAAAAAATACTGACGCGCCGGGCATCCTCAAATAGGAAAACCTGAAGGCACGATTGTTAAATCTCCCACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGCGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCAGAGTGGTAATCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAGCAAAAGGGGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTGGCAAACAAAAATCCTTGCCGCTCATTGATCCCGTTC  >  NC_000913/1344367‑1344853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑