Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,339,929	C→T	G198D (GGT→GAT)	adiA ←	arginine decarboxylase, degradative

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,339,929	0	C	T	100.0%	38.1 / NA	17	G198D (GGT→GAT)	adiA	arginine decarboxylase, degradative
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/10);  total (7/10)

CGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAAC  >  NC_000913/4339695‑4340140                                                                                                                                                                                                                                           |                                                                                                                                                                                                                    cGGCTTGGCACCATAGAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGGTCGATGGGTTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGAGGGTGCGGTGAGAGTCTGAAGTACCGACGACTACCGGCCAGATGCGATCGTCATCAGATACGCGTGCGGCATTTATTTCGCGTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAATCGAGGGAAgttcgttc                                                                                                                                                                                                     <  1:229611/251‑1 (MQ=255)                                                                              aaCGCCCAGGACATCGTTTTCGGTCAGGCAAGCCTGCATGATGTGGCGTTTGGAGCCGTAAGTACCGACGACTACCGACCAGGAGCGATCGTCACCAAATACGCTTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAATCGAGGGAAGTTCGTTCGATGGCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTATAAACGTCCGGCGGGTGTGTTGGTAAAAc                                                                                                                        <  1:111884/251‑1 (MQ=255)                                                                                                  cGGTCATTCAAGCCTGCATGATGGTGCGGTTAGAGCCGGACGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGTCATATTTTTCGCTTTCGCCACATGCGCCAGTATGGTCAAGCAAAGAATCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAAc                                                                                                                        >  1:55650/1‑231 (MQ=255)                                                                                                              ccTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGCCTACCGACCAGGAACGATCGGCAACAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAATCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCAtag                                                                                                                                                                >  1:153871/1‑179 (MQ=255)                                                                                                                                 agagCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAATCGAGGGAAgttcn                                                                                                                                                                                                        <  1:84844/120‑2 (MQ=255)                                                                                                                                    accGGAAGTACCGACGACTACCGACCAGGAGCGATCTGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAATCGAGGGAAGTTCGTTCGATGCCCATGTCGNTGCNNNNNAg                                                                                                                                                                             >  2:200011/2‑144 (MQ=255)                                                                                                                                                  cgacTNCCGGCCAGGAGCGATCGGCACCAAATACGCGNGNGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGANTCGAGGGAAGNTCGNNCGATNCNCNTGTCGGTGCGGAACAGATTTTCACCATAGNAGTCATGGTAGAAACGTCCGGCGNNTGNNNTGNNAAAAc                                                                                                                        <  2:239819/183‑1 (MQ=255)                                                                                                                                                  cgacTACCGTCCAGGAGCGATCGTCACCAAATACGCGAACGGCATATTTTTCGCATTCGCCAACTGCGCCTGTATGGTCGAGCAAGGAATCGAGGGACGTTCGTTATATGCCCATGTCGGTGTGGAACAGATTTTCACCGTAGTAGTCATGGTAGAAACGTCCGGCGGGGGTTTTGGTAAAAc                                                                                                                        >  1:239819/1‑183 (MQ=255)                                                                                                                                                     cTACCGACCAGGAGCGATCGTCACCAAATACACGTGCGTCATATTTGTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAATCGAGGTAAGGTCTTTCGATGCCCATGTCGGTGCGGAAGAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAAc                                                                                                                        <  1:319889/180‑1 (MQ=255)                                                                                                                                                                    gATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGACAAATGCGCCAGTATGGCCCAGCAAAGCATCGAGGGAAGTTCGTTCGATGCCCATGTCGGCGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAACCGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCaa                                   >  1:286044/1‑250 (MQ=255)                                                                                                                                                                                     cgTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAATCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAAAAGCTGCTGGCGGTAGCg                 >  1:15375/1‑251 (MQ=255)                                                                                                                                                                                         cGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAATCGTGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACCGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTNTTGNNNNNAcnn                                                                                                                      >  2:194047/1‑144 (MQ=255)                                                                                                                                                                                           gCATATTTTTCGCTGTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAATCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATagtagt                                                                                                                                                            <  1:179008/106‑1 (MQ=255)                                                                                                                                                                                                 ttttCGCTTTCGCCAAATGCGCCAGTATTGTCAAGCAAAGAATCGAGGGAAGTTCGTTCGATGCTCATGTCGGTGCGGAACAGATTTGCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAATCCAACGCCGCCCTGGTGGCCGTGCGCTTCCCAGTAATATTCATGGATGTCACGATAGTTCATCAGCGCGCTGAGCAGGGTCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCgg      <  1:283271/250‑1 (MQ=255)                                                                                                                                                                                                     cgcttgcgcCAAAAGCGCCAGTATGGTCAAGCAAAGAATCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGTTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCGGCCCAGGAATATTCATGGAGGTCACTATATTTCATCAGCGCGCGGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAAc  <  1:53751/250‑1 (MQ=255)                                                                                                                                                                                                                          tATGGTCAAGCAAAGAATCGAGTGAAGTTCGTTCGATGCCCATGGCGGGGCGTAACAGATGTTCACCATAGTATTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCacc                                                                                                                    <  1:311317/115‑3 (MQ=255)                                                                                                                                                                                                                                cAAGCAAAGAATCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCTCCATAGTATTCATGGTAGAAACGTCCGGCGGGTGGTTTGGTAAAACCAACGCCGCCCTGGTGGCCTCGCGCTTCCCAGGAATATGCATGGATGTCTCTAGATTTCAGCAGCGCGCTGAACAGTGGCGGCAACAgctgatg                          <  1:209521/199‑4 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                    CGGCTTGGCACCATATAGACCGGTTTCGCGCCTGTCAGCATCAAACCTTGTTCGATGGATTTATGGCAGTTACGGTCAACGACCACGACATCGTTATCGGTCATGCAAGCCTGCATGATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAAC  >  NC_000913/4339695‑4340140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑