Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,351,790	G→A	intergenic (‑128/‑53)	dcuS ← / → yjdI	sensor histidine kinase DcuS/PF06902 family protein YjdI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,351,790	0	G	A	100.0%	47.5 / NA	16	intergenic (‑128/‑53)	dcuS/yjdI	sensor histidine kinase DcuS/PF06902 family protein YjdI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/11);  total (5/11)

ACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGTCATGGATCAGGCGCTACTGGACGGGGGTTATCGCTGTTATACCGGCGAAAAGATCGATGTCTATTTCAACACTGCGATATGTCAGCATTCTGGCAATTGCGTACGTGGCAACGGCAAGTTATTTAATCTCAAACGAAAGCCGTGGATCATGCCGGATGAAGTCGAC  >  NC_000913/4351589‑4352007                                                                                                                                                                                                          |                                                                                                                                                                                                                          aCCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAAATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAg                                                                                                                                                                          <  1:22191/251‑1 (MQ=255)                                             tAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGAGGGAGATTCAAAAGGGGGAAGAATGATTGGGTTTATAGGGGAAGAGACTCTGGCAACGGCCACTGCCAGTGCTGTATGAAAATTCCGGGGCTATGCTTATAGCGATAATCATCAtgatga                                                                                                                                                                                    >  1:165497/1‑197 (MQ=255)                                                           gCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGGAAGAATGAGTTGCTATATAGGGGAAGAGACTCTGGCAACTGAAACTTCCAGTTCTGTATGAAAATTCCGGGGCTATTCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGTCAt                                                                                                                                                                     <  1:149794/198‑1 (MQ=255)                                                                                       gtgACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAAATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGTCATGGATCAGGCGCTACTGGACGGGGGTTATCGCTGTTATACCGGCGAAAAGATCGATGTCTATTTCAACACTGCGATATGTc                                                                                     <  1:14852/250‑1 (MQ=255)                                                                                       gtgACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAAATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGTCATGGATCAGGCGCGACTGGACGGGGGTTATCGCTGTTATACCGGCGAAAAGATCGATGTCTATTTCAACACTGCGATATGTc                                                                                     <  1:101477/250‑1 (MQ=255)                                                                                                    tAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAAATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGTCAGGGATCAGGCGCTACTGGACGGGGTTTATCGCTGTTATACCGGCGAAAAGTTCGATGTCTATTTCAACACTGCGATATGTCAGCATTCTGGc                                                                          <  1:171732/248‑1 (MQ=255)                                                                                                                  gatgaGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAAATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGTCATGGATCAGGCGCTACTGGACGGGGGTTATCGCTGTTATACCGGCGAAAAGATCGATGTCTATTTCAACACTGCGATATGTCAGCATTCTGGCAATTGCGTACGTGGc                                                           <  1:9509/249‑1 (MQ=255)                                                                                                                          gggTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAAATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGTCATGGATCAGGCGCTACTGGACGGGGGTTATCGCTGTTATACCGTCGAAAAGATCGATTTCTATTTCAACACTGCGATATGTCAGCATTCTGGCAATTGCGTACGTGGCaan                                                        <  1:38078/244‑2 (MQ=255)                                                                                                                              aaTTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAAATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGTCATGGATCAGGCGCTACTGGACGGGGGTTATCGCTGTTATACCGGCGAAAAGATCGATGTCTATTTCAACACTGCGATATGTCAGCATTCTGGCAATTGCGTACGTGGCAACGGCAAGTTAtt                                             >  1:133707/1‑251 (MQ=255)                                                                                                                               aTTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAAATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGTCATGGATCAGGCGCTACTGGACGGGGGTTATCGCTGTTATACCGGCGAAAAGATCGATGTCTATTTCAACACTGCGATATGTCAGc                                                                                  <  1:9917/213‑1 (MQ=255)                                                                                                                               aTTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAAATTCCGGGGCTANNCTtatan                                                                                                                                                                                                      >  2:9917/1‑96 (MQ=255)                                                                                                                                  cAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGACACTGCCAGTGCTGTATGAAAATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGTCATGGATCAGGCGCTACTg                                                                                                                                                     >  1:53398/1‑143 (MQ=255)                                                                                                                                                aTGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAAATTCCGGGGCTATGCTTATAGCGATAATCATACTTATGAGAGAGGGAAGGTCATGGATCAGGCGCTACTGGACGGGGGTTATCGCGGTTATACCGGCGAAAAGATCGATTTCTATTTCAACACTGCGATATGTCAGCATTCTGGCAATTGCGTACGTGGCAACGGCAAGTTATTTAATCTCAAACGAAAGcc                           <  1:129979/251‑1 (MQ=255)                                                                                                                                                                           ctGGCAACGGAAACTGCCAGTGCTGTATGAAAATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGTCATGGATCAGGCGCTACTGGACGGGGGTTATCGCTGTTATACCGGCGAAAAGATCGATGTCTATTTCAACACTGCGATATGTCAGCATTCTGGCAATTGCGTACGTGGCAACGGCAAGTTATTTAATCTCAAACGAAAGCCGTGGATCATGCCGGATGAAGTCGAc  <  1:10690/249‑1 (MQ=255)                                                                                                                                                                              gcaacngcaacnGNNCGTGCTGTATGAAAATTCCNGGGCTATGCTTATAg                                                                                                                                                                                                      >  2:132747/1‑50 (MQ=255)                                                                                                                                                                              gCAACGGAAACTGCCAGTGCTGTATGAAAATTCCGGGGCTATGCTTATAg                                                                                                                                                                                                      <  1:132747/50‑1 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                                                          ACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGTCATGGATCAGGCGCTACTGGACGGGGGTTATCGCTGTTATACCGGCGAAAAGATCGATGTCTATTTCAACACTGCGATATGTCAGCATTCTGGCAATTGCGTACGTGGCAACGGCAAGTTATTTAATCTCAAACGAAAGCCGTGGATCATGCCGGATGAAGTCGAC  >  NC_000913/4351589‑4352007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑