Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,453,500	C→T	intergenic (‑230/+8)	rpsJ ← / ← gspB	30S ribosomal subunit protein S10/putative general secretion pathway protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,453,500	0	C	T	100.0%	31.2 / NA	15	intergenic (‑230/+8)	rpsJ/gspB	30S ribosomal subunit protein S10/putative general secretion pathway protein B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/8);  total (7/8)

TTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGTTCTTACGTAGCTCCCCGATTGGGAGCATTGTTAGGTAGCCAAATTCGGCTAACTGAGGTTCAGATTGAACCTGCTGTCAACTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCG  >  NC_000913/3453258‑3453715                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                        tttGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGTTCTTACGTAGCTCCCCGATTGGGAGCATTGTTAGGTAGCCAAATTCGGCTAACTGAGGTTCAGATTGAACCTGCTGTCAACTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGTCTTCggg                                                                                                                                                                                                                  <  1:23158/250‑1 (MQ=255)  ttGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGTTCTTACGTAGCTCCCCGATTGGGAGCATTGTTAGGTAGCCAAATTCGGCTAACTGAGGTTCAGATTGAACCTGCTGTCAACTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGTCTTCgggn                                                                                                                                                                                                                 <  1:81760/250‑2 (MQ=255)           cATGAGACCAGATCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGTTCTTTCGTAGCTCCCCGATTGGGAGCATTGTTAGGTAGCCAAATTCGGCTAACTGAGGTTCAGAGTGAACCTGCTGTCAACTACGACAAGCCCGCGCATTAGTCATACTTAACCACCGAGCGCAAGCGTATTGCTAGTAAAGTAAACCGTCTTCGGGTTTGCTCTCaa                                                                                                                                                                                                       <  1:275543/251‑1 (MQ=255)           cATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGTTCTTACGTAGCTCCCCGATTGGGAGCATTGTTAGGTAGCCAAATTCGGCTAACTGAGGTTCAGATTGAACCTGCTGTCAACTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGTCTTCGGGTTATCTCTCa                                                                                                                                                                                                        >  1:153034/1‑250 (MQ=255)                       gCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGTTCTTACGTAGCTCCCCGATTGGGAGCATTGTTAGGTAGCCAAATTCGGCTAACTGAGGTTCAGATTGAACCTGCTGTCAACTACGACAAGCCCGCGCATTATACATACGGAACCACCGAACGCAAGCGTATTGCTAGTAAATTAAACAGTCTTCGGGTTATCTCTCAAGTTCTTTCAGTg                                                                                                                                                                                           <  1:44931/251‑1 (MQ=255)                                                        cctcAGACCCATTACGATTTATGGGATAGTGTAACCGTTCTTACGTAGCTCCCCGATTGGGAGCATTGTTAGGTAGCCAAATTCGGCTAACTGAGGTTAAGATTGAACCTGCTGTCAACTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGTCTTCGGTTTATCTCTCAAGTTCGTTCa                                                                                                                                                                                              <  1:15120/215‑1 (MQ=255)                                                        cctcAGACCCATTACGATTGATGGGAGAGTGTAACCGTTCTTACGTAGCTCCCCGATTGGGAGCATTGTTAGGTAGCCAAATTCGGCTAACTGAGGTTCAGATTGAACCTGCTGTCATCTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGTCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCtttn                                                                                                                                                           <  1:203554/250‑2 (MQ=255)                                                                                gagaGTGTAACCGTTCTTCCGTAGCTCCCCGATTGGGACCATTGTTAGGTACCCCAATTCGGCTAACTGAGGTTCAGATTGAACCTCCTGTCAAATCCGACATGCCCGCGCCTTCTACATACTTAACCACCGAACGCACGCGTATTGCTAGTAAAGTAAACAGTCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTtctc                                                                                                                                                         >  1:295997/1‑228 (MQ=255)                                                                                         aCCGTTCTTACGTAGCTCCCCGATTGGGAGCATTGTTAGGAAGCCAAATTAGGCTAACTGAGGTTCAGATTGAAACTGCTGTCAACTACGACAAGCCCGAGCATTATACATACTTAACAACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGTCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCtt                                                                                                                         >  1:228521/1‑251 (MQ=255)                                                                                                           cccGATTGGGAGCATTGTTAGGTCGCCAAATTCGGCTAACTGAGGTTCAGATTGAACCTGCTGTCAACTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGTCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCAt                                                                                                       >  1:86215/1‑251 (MQ=255)                                                                                                                                            ggCTAACTGAGGTTCAGATTGAACCTGCTGTCAACTCCGACAAGCCCGCCAATTCTAAATACTTAACCACCGCACGCCCGCGTATTGCCCGTAACGTAAACAGTCTTCGGGTTATCTATCAAGTTCGTTCAGCGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCATTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTcctcct                                                                      >  1:161492/1‑251 (MQ=255)                                                                                                                                                                                             gcATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGTCTTCGGGT‑ATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTGCCACAGTATTTTCTTCGGCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAgtgttgt                    <  1:168290/251‑1 (MQ=255)                                                                                                                                                                                              aaTTATACATACTTAACCACCGAACGAAAGCGTATTGCTAGTAAAGTAAACAGTATTCGGGTTATCTCTCAAGTTCGCTCAGTGCATTTTTGACACGCCCTCTTAAACCGGCTTTCTATTCGCTTTCTTACACAGTATTTTATTCGTCTTCAGAAGAAGAggggg                                                                                                          >  1:154651/2‑165 (MQ=255)                                                                                                                                                                                                             accaccGAACGCAAGCGTATTGCTAGTAAAGTAAACAGTCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTggg                                                                                   <  1:41512/173‑1 (MQ=255)                                                                                                                                                                                                                accGAACGCAAGCGTATTGCTAGTAAAGTAAACAGTCTTAGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTATTCGTCTTCAGAAGAAGAGGGGGCTTTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCg  >  1:330470/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                        TTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGTTCTTACGTAGCTCCCCGATTGGGAGCATTGTTAGGTAGCCAAATTCGGCTAACTGAGGTTCAGATTGAACCTGCTGTCAACTACGACAAGCCCGCGCATTATACATACTTAACCACCGAACGCAAGCGTATTGCTAGTAAAGTAAACAGCCTTCGGGTTATCTCTCAAGTTCGTTCAGTGCATTTTTGACACGCTCTCTTAAACCGGCTTTCTCTTCGCTTTCTTCCACAGTATTTTCTTCGTCTTCAGAAGAAGAGGGGGCATTCTCAACCGCGACGCCTGGGTCATCTTCCTCCTCGACGGATTCGACGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCG  >  NC_000913/3453258‑3453715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑