Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,380,354 C→T R55H (CGC→CAC)  frdB ← fumarate reductase iron‑sulfur protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,380,3540CT100.0% 36.7 / NA 14R55H (CGC→CAC) frdBfumarate reductase iron‑sulfur protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/5);  total (9/5)

CCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGC  >  NC_000913/4380150‑4380597
                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   
ncGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGAGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGTGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAgcgc                                                                                                                                                                                                       >  1:96321/2‑251 (MQ=255)
                                                                 gcgTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGTGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGGAGTTGCGTCATAAGGTACTTCATAGAATGCGCTATGCGGTGCGGTATCg                                                                                                                                       >  1:266869/1‑250 (MQ=255)
                                                                                    gCTAACGCTTCAACCTTCATACCGGCGGGGGAATCACGCAGCAAGGTTTTACACGCCAGTTTTGGCACGGTGTTAAGCATCATGCCGCAGGGACCACCAATCGCCATACGGCCGGGCCAGTGGGAGCTCAGGGCCGGTGCCAGGTTGTCTTTTATGTAGCACAGCGCCTCCCGTAATGGGGGAGTTGCGTCATAAGGCACTTCATAGAATACGCTATGCGGGGCGGTATCGACTTCCGGGTTATAGCTcac                                                                                                                   >  1:142546/1‑251 (MQ=255)
                                                                                                     cATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTAGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGTCAGGACCAGTGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATgc                                                                                                                                                          <  1:323737/195‑1 (MQ=255)
                                                                                                                                 gTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGTGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAg                                                                                                    >  1:107105/1‑221 (MQ=255)
                                                                                                                                       cATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGTGGTCGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGCGGTAGTTGCGTcc                                                                                                                                                                              >  1:190236/1‑140 (MQ=255)
                                                                                                                                           ccAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGTGGTAGCTCAGGTCCGGTCCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGgc                                                            >  1:212586/1‑251 (MQ=255)
                                                                                                                                                    ncACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGTGGTAGCTCAGGTCCGGTGCCAgg                                                                                                                                                                                                                              >  1:81146/2‑80 (MQ=255)
                                                                                                                                                             tAACCATCATGCCGCAGGAACCACAAAGCGCCATACGGCAGGACCAGTGGTAg                                                                                                                                                                                                                                                <  1:125900/53‑1 (MQ=255)
                                                                                                                                                                      tGCCGCAGGAACCACAAATCGCCATACGGCAGGACNAGTGNTNNCTNANGTNCGGTg                                                                                                                                                                                                                                   <  2:147462/57‑1 (MQ=255)
                                                                                                                                                                      tGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGTGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGAGGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTGCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTGTCATCTCAGCCATTCGCCTTCTCCTTCTTATTg                                                              <  1:265352/222‑1 (MQ=255)
                                                                                                                                                                                          gCCATACGGCAGGACCAGTGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGAAGGCCAGCGCATCCAGTAATGAGGTCGTTGCCTCATAAGCCACTTCATAGAGTACGGTATGCGGTGCGGGATCGCCTTCCGGGTTCTAGCGCACAACCTCACTTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGtt             >  1:145255/1‑251 (MQ=255)
                                                                                                                                                                                           ccATACGGCAGGACCAGTGGTAGATCAGGTCCGGTGCCAGGATGTCTTTGATGTAGCCCAGCGCATCCAGTAATGCGGTAGTTGCGTCCTAAGGCACTTCATAGAATGCGCTATGCGGTGCGGGATCTACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCCGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGttt            >  1:101152/1‑251 (MQ=255)
                                                                                                                                                                                                     ggACCAGTGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTGTAGCTggcggn  <  1:85589/251‑2 (MQ=255)
                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   
CCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGC  >  NC_000913/4380150‑4380597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑