Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,380	C→T	E87K (GAA→AAA)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,380	0	C	T	100.0%	57.3 / NA	20	E87K (GAA→AAA)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/9);  total (11/9)

GCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACCGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTCGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTCCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAATGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTC  >  NC_000913/3513168‑3513591                                                                                                                                                                                                                     |                                                                                                                                                                                                                    ncTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACCACAAACAGATTAGTTTGATAAACCAGGATGTCCGCTGCCATCAGCACCAGAGACTCAACCAGCCCAGCGTAGATGTTCTCGGCATCACGCGCCGATTTATCTTTAAACTGCGTCATGTGACTCAGTATGCCGACATAGGTATAGCAGTTCAGTGCCCAGCCTAACAGTGCATGTTTCGGCACATGGGACTGAACAAAAATGGTGCTCTtctc                                                                                                                                                                                 >  1:63418/2‑249 (MQ=255)          gAGGTGCTGTTTCTGGTCTTCACCCACCGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCAGCGGATAGTCACACAGAACAGCGTTGATGTTCTCGGCATCACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATAc                                                                                                                                                                      >  1:183757/1‑251 (MQ=255)           aGGTGCTGTTTCTGGTCTTCACCCACCGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTGCTCGGCATAACGCGCAGATTTAGCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGTATCTATAcc                                                                                                                                                                     <  1:264024/251‑1 (MQ=255)                           cTTCACCCACCGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACaa                                                                                                                                                      >  1:18065/1‑250 (MQ=255)                              cacccaccGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCCCCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGAGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGACCAGCCTAACTGTGCATGTTTCGGCACGTGGGCATGAACAAAAATGGTGCTTTTCTCAGGATAGATACCACAAGCCAGATACAAGGcc                                                                                                                                                  >  1:315369/1‑251 (MQ=255)                                     cGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAATGCGCAGATTTATCGTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCgg                                                                                                                                                                                                                  <  1:212000/180‑1 (MQ=255)                                                  gTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCCGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCTTATCCAGCGTCGCttt                                                                                                                              >  1:189234/1‑251 (MQ=255)                                                     tGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTGCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTAc                                                                                                                            <  1:164580/250‑1 (MQ=255)                                                                          nccATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGCTTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTgg                                                                                                        >  1:261205/2‑249 (MQ=255)                                                                            cATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCCGCTTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGgcgc                                                                                                     >  1:119196/1‑250 (MQ=255)                                                                                    ccGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTCTCTTTAAAATGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTCACTGTGCATGTTTCGGCACGTgg                                                                                                                                                                                                           >  1:172952/1‑140 (MQ=255)                                                                                                                      ggCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGTATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGGGATCGCGTGTTGGTCAACGATACAGTAAATGCAATGGt                                                          <  1:153987/251‑1 (MQ=255)                                                                                                                                             tAAACTGCGTCATGCGCCTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCGAACTGTGCATTTTTCGGCACGTCGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATAcc                                                                                                                                                                     <  1:43474/121‑1 (MQ=255)                                                                                                                                                        aTGCGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAATGCAATGGTan                                                        <  1:61471/219‑2 (MQ=255)                                                                                                                                                           cGACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCGCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCtg                                                                                                                  <  1:183143/158‑1 (MQ=255)                                                                                                                                                            gACTCAGTTTGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTCTGCATGTTTCGGCACGTGGCACTGAAAAAAAATGGTACTTTTATCAGGCTCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGc                                                                                                                                 >  1:321497/1‑142 (MQ=255)                                                                                                                                                                       cagAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACACAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGTCTCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAATGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTAcc         >  1:330661/3‑251 (MQ=255)                                                                                                                                                                              aGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAATGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTc  <  1:259299/251‑1 (MQ=255)                                                                                                                                                                                                          cTGTGCATGTTTCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATCCCACAACCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAATGCAATg                                                            >  1:177981/1‑165 (MQ=255)                                                                                                                                                                                                             tgCATGTTTCGGCTCGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCTCAAGCCAGATACAAAGCCAGCGTATCCAGCGGCGCTTTACGCAGCTTCTGTTCATCCTGGCGCACGGTGTTCGCGGGTTGGTCAAGGATGCAGTAAATGCAATGGTAGGCATCCTGCATGTTTACCCACTGACGCAgcg                         <  1:216035/197‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                    GCTCAGTTCGAGGTGCTGTTTCTGGTCTTCACCCACCGGTACCAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATCAGCACCGGATAGTCAAACAGACCAGCGTTGATGTTCTCGGCATAACGCGCAGATTTATCTTTAAACTGCGTCATGCGACTCAGTTCGCCGAAGTAGGTATAGCAGTTCAGTGCCCAGCCTAACTGTGCATGTTCCGGCACGTGGGACTGAACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAATGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTC  >  NC_000913/3513168‑3513591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑