Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,894,178	G→A	G100S (GGC→AGC)	yieE →	putative phosphopantetheinyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,894,178	0	G	A	100.0%	33.4 / NA	13	G100S (GGC→AGC)	yieE	putative phosphopantetheinyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/5);  total (8/5)

CGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTGGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCCCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCT  >  NC_000913/3894018‑3894404                                                                                                                                                                 |                                                                                                                                                                                                                                   cGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTAGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCCCGATAACCACACCTTTTCCAGCAATGAATCGCTAt                                                                                                                                          >  1:279631/1‑251 (MQ=255)          gATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTGCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTAGCCTCGATATGGAACTACAGCGTTCGACGCGCGGGTGTCATAGCCCACACGCGCCCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAg                                                                                                                                   <  1:149069/249‑1 (MQ=255)                          ggCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTAGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCCCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTa                                                                                                                  >  1:159851/1‑250 (MQ=255)                                                    naCCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTAGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATa                                                                                                                                                                                          >  1:192206/2‑152 (MQ=255)                                                      nnnnnncGNNNNNTAANCCGTTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTCCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTAGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATa                                                                                                                                                                                          <  2:192206/144‑1 (MQ=255)                                                                          gTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTAGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGtttn                                                                                                                                                                                             <  1:79545/127‑2 (MQ=255)                                                                                                              tCCTATGCCGGGAATATGGTTGGCGTGCCTTTAACAACCCAAGGTGAATGTAGCCTCGATATGGAACTACAGCGTCCTACGCGCGCGTTTCAGGGCCAACACGCGCCCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAAc                                                                                                                              <  1:161623/154‑1 (MQ=255)                                                                                                                  aTGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAAGGTAGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCCCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAg                         <  1:168023/251‑1 (MQ=255)                                                                                                                                    gcgtggcgtTAACAACCGAAGGTGAATGTAGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATCGCCCACACGCGCCCGATAACCCCACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAAAGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAAc                                                              >  1:264619/1‑196 (MQ=255)                                                                                                                                         gcgtTAACAACCGAAGGCGAATGTAGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCCCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGTGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCt  >  1:252182/1‑251 (MQ=255)                                                                                                                                          cgtTAACAACCGAAGGTGAATGTAGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCAAAGCCCACAAGCGCCAGATAACCACACCTTTTCAAGCAATGACTAGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCAAGCTGCGCCGAAGCGTGCTAAAACTACCCGGTg                                                         >  1:208188/1‑195 (MQ=255)                                                                                                                                                   ccGAAGGTGAATGTAGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCCCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGANGCGNNNnngcnn                                                                                                 >  2:196998/1‑144 (MQ=255)                                                                                                                                                    cGAAGGTGAATGTAGGATAGATATGGAACTAACGAGTGCGACGCGCGGGTTTAATAGCCCACACGCGACCGATAACCACACCTTTTCCAGCAATGAATAGCTATGGACCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATc                              >  1:115654/1‑212 (MQ=255)                                                                                                                                                                 |                                                                                                                                                                                                                                   CGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTGGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCCCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCT  >  NC_000913/3894018‑3894404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑