Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,072,358	G→A	G274S (GGC→AGC)	flu →	self recognizing antigen 43 (Ag43) autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,072,358	0	G	A	100.0%	30.1 / NA	12	G274S (GGC→AGC)	flu	self recognizing antigen 43 (Ag43) autotransporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/7);  total (5/7)

ATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCGGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACTGTTGTGAACAGTGACGGCTGGCAGATTGTCAAAAACGGGGGTGTGGCCGGGAATACCACCGTTAATCAGAAGGGCAGACTGCAGGTGGACGCCGGTGGTACAGCCACGAATGTCACCCTGAAGCAGGGCGGCGCACTGGTTACCAGTACGGCTGCAACCGTTACCGGCATAAACCGCCTGGGAGCATTCTCTGTTGTGGAGGGTAAAGCTGATAATGTCGTACT  >  NC_000913/2072184‑2072566                                                                                                                                                                               |                                                                                                                                                                                                                 aTCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCGGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGGATGTGCACAACGGCGGTACAGCGTCTGACACTGTGGTGAACAGTGACAGCTGGCAGATTTTCAAATACGGGGGTGTGGCCGGGAATACCACCGTTAATCAGAAGGGCAGACTGCAGGTGGACGc                                                                                                                                      <  1:154254/251‑1 (MQ=255)       aaaaaCGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCGGCGACCAGACTGTACATGGTCACGCACTTGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACTGTTGTGAACAGTGACAGCTGGCAGATTGTCAAAAACGGGGGTGTGGTCGGGAATACCACCGTTAATCAGAAGGGCAGACTGCAGGTGGACGCCggtgg                                                                                                                                <  1:122356/251‑1 (MQ=255)                                                       gtgGTTTATGCCGGCGGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACTGTTGTGAACAGTGACAGCTGGCAGATTGTCAAAAACGGGGGTGTGGCCGGGAATACCACCGTTAATCAGAAGGGCAGACTGCAGGTGGACGCCGGTGGTACAGCCACGAATGTCACCCTGAAGCAGGGCGGCGCACTGGTTACCan                                                                                <  1:57931/251‑2 (MQ=255)                                                                 tcgtcggcgACCAAAGTGTGCATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATTTGCACGATGGCGGTACAGCGTCTGACACTGTTGTGAACAGTGATAGCTGGCAGATTGTCAAAAACTGGGGTGTGTCCGGGAATACCACCGTTAATCAGAAGGGCAGACTGCAGGTGGACGCCggtggt                                                                                                                               <  1:160267/190‑1 (MQ=255)                                                                       gcgACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAACGTCTGACACTGTTGTGAACAGTGACAGCTGGCAGATTGTCAAAAACGGGGGTGTGGCCGGGAATACCACCGTTAATCAGAAGGGCAGACTGCAGGTGGACGCCGGTGGTACAGCCACGAATGTCACCCTGAAGCAGGGCGGCGCACTGGTTACCAGTACGGCTGCAACCGtt                                                                >  1:264291/1‑251 (MQ=255)                                                                               aCTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACTGGTGGGAACAGTGACAGCTGGCAGATTGTCAAAAACGGGTTTGTGGCCTGGAATACCACCGTTAATCAGAAGTGCAGACTGCAGGTGGACGCCGGTGGTACAGCCACGAATGTc                                                                                                                <  1:147470/195‑1 (MQ=255)                                                                                                            gCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGAGTCTGACACTGTCGTGAAAAGTGAAAGCTGGAAGCCTGTCAAAACCGGGGGTGTGACCGGGAATACCACCCTTAATCAGCAGGGCAGACTGCCGGTGGACGCCGGTGCTACAGCCCCGCATGTCACCCTGCAGCCGGGCGCCGCACTGGTTACCAATACAGCCGCAACCGTTACAGGCATAAACCGCCAGGGCGAATTCTCTGTTGTgg                           >  1:159202/1‑251 (MQ=255)                                                                                                                           ccAGTATGTGCACAACGGCGGTACAGGGTCTGACACTGTTGTGAACAGTGACAGCTGGCGGATTGTCAAAAACGGGGGTGTGGCCGGGAATACCACCGTTACTCAGACGGGCAGACTGAAGGGGGACGCCGGTGGTACAGCCACGAATGTCACCCTGAAGCAGGGCGGCGCACTGGTTACCAGTACGGCTGCAACCGTTACCGGCATAAACCGCCTGGGAGCATTctct                                  >  1:242622/1‑229 (MQ=255)                                                                                                                             aGTATGTGCACAACGGCGGTACAGCGTCTGACACTGTTGTGAACAGTGACAGCTGGCAGATTGTCAAAAACGGGGGTGTGGCCGGGAATACCACCGTTAATCAGAAGGGCAGACTGCAGGTGGACGCCGTTGGTACAGCCACGAATGTCACCCTGAAGCAGGGCCGCGCCCTGGTTAcc                                                                                  <  1:259627/179‑1 (MQ=255)                                                                                                                                     cacaACGGCGGTACAGCGTCTGACACTGTTGTGAACAGTGCCAGCTGGCAGATTGTCAAAAACGGGGGGGTGGCCGGGAATACCCCCGTTAATCCGAAGGGCAGACTGCCGGTGGACGCCGGTTGTACAGCCACGAATGTCACCCTGAAGCAGGGCGGCGCACTGGTTACCCGTACGGCTGCAACCGTTACCGGCAt                                                        >  1:147117/1‑197 (MQ=255)                                                                                                                                     cacaACGGCGGTACAGCGTCTGACACTGGTGTGAACAGTGACAGCTGGCAGATTGTCAAAAACGGGGGTGTGGCCGGGAATACCACCGTTAATCAGAAGGGCAGACTGCAGGTTGACGCCGGTGGTACAGCCACGAATGTCACCCTGAAGCAGGGCGGCGCACTGGTTACCAGGACGGCTGGAACCGTTACCGTCATAAACCGCCTGGGAGCATTCTCTGTTGTGGAGGGTAAAGCTGATAATGTCGTACt  <  1:159865/251‑1 (MQ=255)                                                                                                                                                  cAGCGTCTGACACTGTTGTGAACAGTGACAGCTGGCAGATTGTCAAAAACGGGGGTGTGGCCGGGAATACCACCGTTAATCAGAAGGGCAGACTGCAGGTGGACGCCGGGGGTACAGCCACGAATGTCACCCTGAAGCAGGGCGGCGCACTGGTTACCAGTACGGCTGCAACCGTTACCGTCATAAACCGCCTGGGAGCATTCTCTGTTTTGGAGGGTAAATCTGATaa           >  1:2929/1‑229 (MQ=255)                                                                                                                                                                               |                                                                                                                                                                                                                 ATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCGGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACTGTTGTGAACAGTGACGGCTGGCAGATTGTCAAAAACGGGGGTGTGGCCGGGAATACCACCGTTAATCAGAAGGGCAGACTGCAGGTGGACGCCGGTGGTACAGCCACGAATGTCACCCTGAAGCAGGGCGGCGCACTGGTTACCAGTACGGCTGCAACCGTTACCGGCATAAACCGCCTGGGAGCATTCTCTGTTGTGGAGGGTAAAGCTGATAATGTCGTACT  >  NC_000913/2072184‑2072566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑