Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,852,006	C→T	C40C (TGC→TGT)	pncA →	nicotinamidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,852,006	0	C	T	100.0%	20.9 / NA	9	C40C (TGC→TGT)	pncA	nicotinamidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/6);  total (3/6)

GCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACGCCGGATGATTAAGGAGACTGTAATGCCCCCTCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGCCGGAAGGTGACAGTACGGTGGATGTCGCTAACCGCCTGATTGACTGGTGCCAGTCGCGCGGTGAAGCGGTTATCGCCAGTCAGGACTGGCACCCGGCGAATCACGGCAGTTTTGCCAGTCAGCACGGTGTAGAGCCTTATACGCCAGGCCAACTCGACGGTTTGCCACAAACCTTCTGGCCAGATCACTGTGTGCAGAACAGTGAAGGCGCACAATTACATCCGTTACTGCACCAAAAAGCGATCGCAGCGGTGTTCCATAAAGGCGAAAATCCTTTAGT  >  NC_000913/1851769‑1852236                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       gCCACGCTAACCAAACTGCATGACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACGCCGTATGATTAAGGATACTGTAATGCCCCCCCGCGCCCTGTTACTGGTCGATTTACAAAATGAGTTCTGTGCTGTTGGCGCGCTCGCCGTGCCGGAATGTGACAGTACGGGGGATGTCGCTAACCGCCTGATTGACTGGTGTCAGTcg                                                                                                                                                                                                                                  <  1:158327/244‑1 (MQ=255)    aCGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACGCCGGATGAGAAAGGAGACTGTAATGCCCCCTCGCGCCCTGGGACTGGTCTATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCTCCGTTCCGGAAGGTGACAGGTCGGTGGATGTCGCTAACCGCCTGATGGACTGGTGTCAGTCGCGCGGTGAAg                                                                                                                                                                                                                        <  1:330196/251‑1 (MQ=255)                                                                  cattaGCCAAAACCTGCGCGGCGAACTGACGCCGGATGAGTAAGGAGACTGTAATGCCCCCGCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCGGTGCGGGTGGCGGGCTCGCCGTGCCGGAAGGTGACAGTACGGTGGATGTCGCTAACCGCCTGAGTGACTGGTGTCAGTCGCGCGGTGAAGCGGTTGTCGCCAGGCAGGACTGGCACCCGGCGAAGCACGGCAGTTTTGCCAGTc                                                                                                                                                                  <  1:220169/239‑1 (MQ=255)                                                                                 gcgcGTCGAACTGACGCCGGATGATTAAGGAGACTGTAATGCCCCCTCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGCCGGAAGGGGACAGTACGGTGGATGTCGCTAACCGCCTGATTGACTGGTGTCAGTCGCGCGTTGAAGCTGTTATCGCCAGTCAGGACTGGCACCCGGCGAATCACTGCAGTTTTGCCAGTCAGCACGGTGTAGAGCCTTATAc                                                                                                                                            <  1:243503/250‑1 (MQ=255)                                                                                    cggcgAACTGACGCCGGATGATTAAGGAGACTGGAATGCCCCCTCGCGCCCTGGTACTGGTCTATTTACAAAATGATTTCTGTGCTGGTGGCGCCCTCGCCGTGCCGGAAGGAGGCAGTACGGTGCATGTCGCTAACCTCCTGATTGACTGGTGTCAGTCGCGCGGTGAAGCGGTTATc                                                                                                                                                                                                                <  1:307730/179‑1 (MQ=255)                                                                                                                                              ggTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGCCGGAAGGTGCCAGTACGGTGGATGTCGCTAACCGCCTGATTGACTGGTGTCAGTCGCGCGGTGAAGCGGTTATCGCCCGTCAGGACTGGCACCCGGCGAATCACGGCAGTTTTGCCAGTc                                                                                                                                                                  >  1:98754/1‑167 (MQ=255)                                                                                                                                              ggTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGCCGGAAGGTGACAGTACGGTGGATGTCGCTAACCGCCTGATTGACTGGTGTCAGTCGCGCGGTGAAGCGGTTATCGCCAGTCAGGACTGGCACCCGGCGAATCACGGCAGTTTTGCCAGTCAGCACGGTGTAGAGCCTTATACGCCAGGCCAACTCGACGGTTTGcc                                                                                                                    <  1:249557/213‑1 (MQ=255)                                                                                                                                                                                                                ggATGTCGCTAACCGCCTGATTGACTGGTGTCAGTCGCGCGGTGAAGCGGTTATCGCCAGTCAGGACTGGCACCCGGCGAATCACGGCAGTTTTGCCAGTCAGCACGGTGTAGAGCCTTATACGCCAGGCCCACTCGACGGTTTGCCACACACCTTCTGGCCAGCTCACTGTGTGCCGAACAGTGa                                                                             >  1:44899/1‑186 (MQ=255)                                                                                                                                                                                                                          caCCGCCTGATTGACTGGTGTCAGTCGCGCGGTGAAGCGGTTATCGCCAGTCAGGACTGGCACCCGGCGAATCACGGCAGTTTTGCCAGTCAGCACGGTGTAGAGCCTTATACGCCAGGCCAACTCGACGGTTTGCCACAAACCTTCTGGCCAGATCACTGTGTGCAGAACAGTGAAGGCGCACAATTACATCCGTTACTGCACCAAAAAGCGATCGCAGCGGTGTTCCATAAAGGCGAAAATCCTTTAGt  >  1:92349/2‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       GCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACGCCGGATGATTAAGGAGACTGTAATGCCCCCTCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGCCGGAAGGTGACAGTACGGTGGATGTCGCTAACCGCCTGATTGACTGGTGCCAGTCGCGCGGTGAAGCGGTTATCGCCAGTCAGGACTGGCACCCGGCGAATCACGGCAGTTTTGCCAGTCAGCACGGTGTAGAGCCTTATACGCCAGGCCAACTCGACGGTTTGCCACAAACCTTCTGGCCAGATCACTGTGTGCAGAACAGTGAAGGCGCACAATTACATCCGTTACTGCACCAAAAAGCGATCGCAGCGGTGTTCCATAAAGGCGAAAATCCTTTAGT  >  NC_000913/1851769‑1852236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑