Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,434,299	C→T	L63F (CTT→TTT)	ytfH →	putative transcriptional regulator YtfH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,434,299	0	C	T	100.0%	27.9 / NA	13	L63F (CTT→TTT)	ytfH	putative transcriptional regulator YtfH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/10);  total (3/10)

GTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGC  >  NC_000913/4434117‑4434534                                                                                                                                                                                       |                                                                                                                                                                                                                                            gTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGTTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTc                                                                                                                                                                                                      >  1:129754/1‑222 (MQ=255) gTCAGGGTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGTTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCggtgg                                                                                                                                                                              <  1:234108/246‑1 (MQ=255)                                                                   aGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTTATTCTGGTGGCGCTACTCGAAGGTACTCATCGCTTTAGCGACCTTCTGCGCAAAATCGGTGGGGTGAGTGAAAAGATGTTTGCGCAGGCGTTACAGTCTTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTTGTGCCGCCGCATGTGTAATATAGCCTCACGTCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCg                                                                                                       <  1:300140/251‑1 (MQ=255)                                                                         tGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGTTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGGATCGCGTATCCGGTGGTGCcgccgc                                                                                                                                                                     <  1:97960/183‑1 (MQ=255)                                                                                        gCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGAGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGTTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAg                                                                                                                                                        >  1:296510/1‑181 (MQ=255)                                                                                                              ggtggCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGTTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGTTGTTGGTGGTGCgg                                                             <  1:88902/250‑1 (MQ=255)                                                                                                              ggaggCGCTACGCGGAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGTTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGCAAAGGTTGCTGCACTGGCg                                                                                                       >  1:26510/4‑208 (MQ=255)                                                                                                                  gcgcTACGCGAAGGTACTCATCGCTTTAGCGACCGGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGTTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGgtggtg                                                                                                                                                                            <  1:320644/135‑1 (MQ=255)                                                                                                                    gcTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGTTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAAc                                                      <  1:273729/251‑1 (MQ=255)                                                                                                                    gcTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGTTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAAc                                                      <  1:273731/251‑1 (MQ=255)                                                                                                                                             aGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGTTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATgcgc                              <  1:106957/250‑1 (MQ=255)                                                                                                                                                              aaTCTGTGGGGTGAGTGAAAAGATGTTTGCGCAGTCGTGACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTgcc                                                                                                                                                                          <  1:45175/93‑1 (MQ=255)                                                                                                                                                                        gtgagtgaAAAGATGTTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGc  <  1:4067/251‑1 (MQ=255)                                                                                                                                                                                       |                                                                                                                                                                                                                                            GTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGC  >  NC_000913/4434117‑4434534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑