Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,325,598	G→A	C48C (TGC→TGT)	yjdN ←	PF06983 family protein YjdN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,325,598	0	G	A	100.0%	37.4 / NA	15	C48C (TGC→TGT)	yjdN	PF06983 family protein YjdN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/10);  total (5/10)

AAGCCATGCGCCCAGAAAGTTTCCTGCCAGGCCATTTCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGG  >  NC_000913/4325368‑4325836                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               aaGCCATGCGCCCAGAAAGTTTCCTGCCAGGCCATTTCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTGTCCTTCTTCGACCTGGTGCGAATCGAGCACCAGCGTAAAGCCTGAGTAGCTTGCTTTTCCTGACGGCAGGGCATCGCTCATCAGGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGACAGTTCTCGGCGCTGTCCtn                                                                                                                                                                                                                            <  1:60593/251‑2 (MQ=255)                            cAGGCCATTTCGATTTTTACGTTAGCGGCAAGATTGTCAAAGCAGCGTTATCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGCGTCGCTGGCTTTTCCTGACGGCATGGCATCGATCATCATGATGTCGCTTCCGGCCATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCAGAAGGACAGTTCTCGGCGGTGTCCTGCGTTGATTTTGGCATTTCGGCGAAGCt                                                                                                                                                                                                 >  1:119980/1‑251 (MQ=255)                               gCCATTTCGATTTTTCCGTTAGCTGCAAGATTGTCAAACCAGCTTTTTCCTTCTTCGACCTGTTGCGAAGCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCGTTTTCTGACGGCATGGTTTCGCTCATCTTGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGAGGGCGTTATCGGGAAATTGCATTCCGGAAGGACAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGAt                                                                                                                                                                                              <  1:314609/251‑1 (MQ=255)                                       gATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCGTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATGGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGACAGTTCTCGgc                                                                                                                                                                                                                                     <  1:123082/204‑1 (MQ=255)                                        aTTTTTCCGTGAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGTAAGGACAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGAt                                                                                                                                                                                              <  1:126833/242‑1 (MQ=255)                                                                                                             aGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATTATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGGGGTATCGGGAAATTGCATTCCGGAAGGACAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTa                                                                                                                <  1:88835/251‑1 (MQ=255)                                                                                                                        gggggatcTGGCTTTTCCTGACGGCATGGGATCGCTCATCCTGATGTCGCTTCCGGCAATGCGCACAGTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGACAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATGTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGTACGTAACg                                                                                                     <  1:77664/244‑1 (MQ=255)                                                                                                                               cTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGACAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCatgat                                                                                              >  1:272011/1‑251 (MQ=255)                                                                                                                                                             atcatGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGACAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGTAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAg                                                                <  1:293772/251‑1 (MQ=255)                                                                                                                                                                        gCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGACAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGGGTAGTTCGa                                                      >  1:245706/1‑250 (MQ=255)                                                                                                                                                                               gCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGAAAGTACTCGGCGCTGTCATGCGATGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGCACGTTGATAATCGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCACGTTAAGTGTAGTTCGAAATTtat                                               >  1:242905/1‑250 (MQ=255)                                                                                                                                                                                  aTGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGACAGCTACCGACGCTGTCCTGCGATGAATTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGAAAACGCGTCGGAACAGTTAACGGAAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATAc                                             >  1:178721/1‑249 (MQ=255)                                                                                                                                                                                              gCGTGGGCGATGGCGGTATCGGGAAAGTGCATTCCGGAAGGACAGTTCTCGGCTCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTGTATAGAGCAGTTCCGCGCCCAACGTACGGTGATAATAGGCAATCGCGTCGGAACAGGTTCCGGCAAAAGAGAGGTAGGGAGTGAACTTCATGATGGTGACTTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAggcggc                               <  1:163737/251‑1 (MQ=255)                                                                                                                                                                                                           cGGTATCGGGAAATTGCATTCCGGAAGGACAGTTTTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGAGGGTTACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGAGGAGAGGCGGCCTGATAAGAcgcg                  <  1:109684/251‑1 (MQ=255)                                                                                                                                                                                                                           cATTCCGGAAGGACAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAgg  <  1:309518/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               AAGCCATGCGCCCAGAAAGTTTCCTGCCAGGCCATTTCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGG  >  NC_000913/4325368‑4325836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑