Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,268,970	A→G	T186A (ACA→GCA)	yhaC →	uncharacterized protein YhaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,268,970	0	A	G	100.0%	54.6 / NA	16	T186A (ACA→GCA)	yhaC	uncharacterized protein YhaC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/9);  total (7/9)

CCATTAATGAACCGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCC  >  NC_000913/3268737‑3269136                                                                                                                                                                                                                                          |                                                                                                                                                                       ccATTAATGAACCGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGcc                                                                                                                                                       >  1:295024/1‑251 (MQ=255)      aaTGAACCGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATAGTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGAGATCGTTGATCTTTCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCatca                                                                                                                                                   <  1:296011/250‑4 (MQ=255)            ccGTTAAAAAACCAGGAAATATCCACAGGTAATATAAATATTAATTGCCCACTAAAAGATATTTACAATGAAATCAGGAGGATAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGAATTACGTAATGACGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTc                                                                                                                                             >  1:191153/1‑250 (MQ=255)                              atatCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATTGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAAGTTGACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTc                                                                                                                          <  1:240228/251‑1 (MQ=255)                                                             cNTTNNAAGANATTTACAATGAAATCAGGAGGTTANACGTAATTTTTAGTTGTGGGACTGGATATATNGNTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGANTTCACAGATANACANNTGGCNANTNCTAGTTTAAATCCTTTTAAATTGAATTCANCAAAGTTTACTAATGCCAACATGNNTCNNNTTNNCTTTg                                                                                                                                 <  2:234711/213‑1 (MQ=255)                                                              aTTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGattatt                                                                                          >  1:161943/1‑251 (MQ=255)                                                                  aaaGATATTTACAATGAAAGCAGGATGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATAGCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAAGTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACACCACAATCTCCTGGGATTATTTACt                                                                                      <  1:216383/251‑1 (MQ=255)                                                                                                naCGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCCCAGATAAACATATGGATAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGc                                                                                                              >  1:61324/2‑197 (MQ=255)                                                                                                 aCGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCGCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATGTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                       <  1:15402/251‑1 (MQ=255)                                                                                                               tgtgGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATGAGCGATATGTATTCtn                                          <  1:95749/250‑2 (MQ=255)                                                                                                                                           ccTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATGTCACAGAGAAACATATGGCTAATACTATTGTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAgg                                                                                                                                          <  1:165975/126‑1 (MQ=255)                                                                                                                                                       tACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGcn  <  1:79388/250‑2 (MQ=255)                                                                                                                                                       tACGTAATGTCGATTCAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGc   <  1:49902/249‑1 (MQ=255)                                                                                                                                                                                          gATAAACATATGGCTAATACTATTTTAAATGCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAgttg              >  1:274669/1‑203 (MQ=255)                                                                                                                                                                                                               aTTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCan                                                                                                                                           <  1:35260/57‑2 (MQ=255)                                                                                                                                                                                                               aTTTTAAANCCTTNTNNNTTGAANTCAGCAAATNTTACTAATGCCAACATGTTNCAg                                                                                                                                           >  2:35260/1‑57 (MQ=255)                                                                                                                                                                                                                         cTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCCACACACACCGCCACAATCTCCTGGGATTATTTACTAAAAATAAAGCCTGTTTTACTAAg                                                              >  1:297737/1‑124 (MQ=255)                                                                                                                                                                                                                               caTTGNATTCANCNNCTTTTACTCATGCCAACATNGTTCAGGTTAATTTTNTTCGNTCAacnc                                                                                                                     >  2:137000/2‑63 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                       CCATTAATGAACCGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCC  >  NC_000913/3268737‑3269136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑