Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,603,942	C→T	S32S (TCC→TCT)	hyfC →	hydrogenase 4 component C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,603,942	0	C	T	100.0%	10.1 / NA	7	S32S (TCC→TCT)	hyfC	hydrogenase 4 component C
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/5);  total (2/5)

GGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTAT‑GTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTATGCACTCCCGCCGCGGGCCGGGGATCTGGCAGGATTATCGCGATATCCACAAACTGTTTAAACGCCAGGAAGTTGCGCCGACATCTTCAGGTCTGATGTTCCGCCTGATGCCGTGGGTATTAATCAGCAGCATGCTGGTGCTGGCGATG  >  NC_000913/2603700‑2604110                                                                                                                                                                                                                                                    |                                                                                                                                                                         gggTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTAT‑GTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGGTAGCACAGGCCGTGATTCGGCTGATGCTAACCCCACTTTTTACGGGTATTTCTCGGCAg                                                                                                                                                                    <  1:265694/249‑1 (MQ=255)                                                     gcaccTGCAAAGCGGCGTCTTTCGTCTCTAGTACCTGTATCGTG‑TCCCCGCACTGGTTGTGCGGCTAATCGCGATTGCCGTCAAAGGAAATCACCATGAGACAAACTCTTTGCTACGGATATGTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCGAACCCCGCCTTTGACGGGTATTTCTCGTCATATACTCGCGCGTAGGCACTCCCGCCGCGGACCGGGGATCTGGCAGGATTATcgc                                                                                                              <  1:300825/247‑1 (MQ=255)                                                                                               gggTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCATGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCTCGGCAGATACGCGCGCGTATGCACTCCCGCCGCGGGCCGGGGATCTGGCAGGATTATCGCGATATCCACAAACTGTTTAAACGCCAGGAAGTTGCGCCGAc                                                                     <  1:251714/250‑1 (MQ=255)                                                                                                                                  gCCGTCTAAGGATATCACCATGAGCCAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCTCGGCAGATACGCGCGCGTATGCACTCCCGCCGCGGGCCGGGGATCTGGCAGGATTATCGCGATATCCACAAACTGTTTAAACGCCAGGAAGTTGCGCCGACATCTTCAGGTCTGATGTTCCGCCTGATGCCGTggn                                  <  1:85825/251‑2 (MQ=255)                                                                                                                                    cGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGTTATTTCTCGGCAGATACGCGCGCGTATGCACTCCCGCCGCGGGCCGGGGATCTGGCAGGATTATCGCGATATCCACAAACTGTTTAAACGCCAGGAAGTTGCGCCGACATCTTCAGGTCTGATGTTCCGCCTGATGCCGTGGGTa                                <  1:114338/251‑1 (MQ=255)                                                                                                                                              naTCACCATGAGACAAACTCTTTGCGACGGATCTCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCTCGGCAGATACGCGCGCGTATGCACTCCCGCCGCGGGCCGGGGATCTGGCAGGATTATCGCGATATCCACAAACTGTTTAAACGCCAGGAAGTTGCGCCGACATCTTCAGGTCTGATGTTCCGCCTGATGCCGTGGGTATTAATcagc                       >  1:96558/2‑250 (MQ=255)                                                                                                                                                                                                                     tgATGCTAACCACCCTTTTTACGGGGATTTCTCGGCAGATACGCGCGCGTATGCACTCACGCCGCGGGTCGGGGATATGGGCGGATTATCGCGATATCCACAAACTGTTTAAACGACAGGAAGTTGCGCAGACATCTTCAGGTCTGATGTTCCGCCTGATGCCGTGGGTCTTAATCAGCAGCATGCTGGAGCTGGCGATg  >  1:265002/1‑200 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                         GGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTAT‑GTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGTATGCACTCCCGCCGCGGGCCGGGGATCTGGCAGGATTATCGCGATATCCACAAACTGTTTAAACGCCAGGAAGTTGCGCCGACATCTTCAGGTCTGATGTTCCGCCTGATGCCGTGGGTATTAATCAGCAGCATGCTGGTGCTGGCGATG  >  NC_000913/2603700‑2604110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑