Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	677,723	C→T	C103C (TGC→TGT)	djlB →	J domain‑containing protein DjlB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	677,723	0	C	T	100.0%	29.5 / NA	12	C103C (TGC→TGT)	djlB	J domain‑containing protein DjlB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

TATCTGGCGCTGTTACCGTCCTTTCATCCAGAAACCGATCCGCAGGGTTTTAAACAACTTCGTCAGGCGTATGAGGAGGCGCTACGGATTGCGCAGTCGCCTGCTAAATCTGTTTGGCAACCAGAAGAATATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGC  >  NC_000913/677481‑677890                                                                                                                                                                                                                                                   |                                                                                                                                                                        tATCTGGCGCTGTTACCGTCCTTTCATCCAGAAACCGATCCGCAGGGTTTTAAACAACTTCGTCAGGCGTATGAGGAGGCGCTACGGATTGCGCAGTCGCCTGCTAAATCTGTTTGGCAACCAGAAGAATATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGTTCGATgg                                                                                                                                                                  <  1:75746/250‑1 (MQ=255)                                                                                                       gCTAAATCTGTTTGGCAACCAGAAGAATATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGTTCGATGGAGGAGATTGATGAATTAc                                                                                                                                                >  1:109220/1‑166 (MQ=255)                                                                                                                     gCAACCAGAAGAATATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGTTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGca                                                                                                                                 <  1:291514/167‑1 (MQ=255)                                                                                                                     gCAACCAGAAGAATATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGTTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAAt                                              >  1:154762/1‑250 (MQ=255)                                                                                                                          cAGAAGAATATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCATCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGTTCGATGGAGGAGATTGATGAATTACGCTGGTCGCtgtg                                                                                                                                   >  1:151252/1‑160 (MQ=255)                                                                                                                                             ngAACATGCAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGCTTTCTGCCCTCCGCCTGGCAGAGATTCATTCAGCAATTAAATTATTGTTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAGATAGACGAAGAAGAAc                     >  1:83851/2‑251 (MQ=255)                                                                                                                                                 cATGAAATTCTGCTCGCCTTTCGTGCTTTACTTTCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGTTCGAGGGAGGAGATTGATGAATTACGCTTGGCGCTGTGCACAATCGCCATGAACTCTGCCGAGTTAGCCTTCGATTTCGTTGTTTTATTAGCAGTAAGATTGCGGTGTTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGa                 <  1:321218/251‑1 (MQ=255)                                                                                                                                                                gCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGTTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGc  <  1:281126/251‑1 (MQ=255)                                                                                                                                                                         gCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGTTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATGAGCAGAAAGATTGCGGTGGttn                                                          <  1:93991/186‑2 (MQ=255)                                                                                                                                                                                    ctctGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGAAATTAAATTCTTGTTGGATGGAGGAGATTGATGTATTAAGCTCATCGCTCTGCACAATCGCCATGAACACTGc                                                                                                              >  1:324952/1‑123 (MQ=255)                                                                                                                                                                                        gATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGTTCGATggagg                                                                                                                                                               <  1:179038/70‑1 (MQ=255)                                                                                                                                                                                                     tnctncCCTCNGCCTNGCAGCGATTCATTCANCAATGAAAATATTGTTCGGNNGNGGAGANtgatg                                                                                                                                                      <  2:132973/66‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                        TATCTGGCGCTGTTACCGTCCTTTCATCCAGAAACCGATCCGCAGGGTTTTAAACAACTTCGTCAGGCGTATGAGGAGGCGCTACGGATTGCGCAGTCGCCTGCTAAATCTGTTTGGCAACCAGAAGAATATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGC  >  NC_000913/677481‑677890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑