Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,441,859	C→T	K80K (AAG→AAA)	rpsK ←	30S ribosomal subunit protein S11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,441,859	0	C	T	100.0%	48.0 / NA	17	K80K (AAG→AAA)	rpsK	30S ribosomal subunit protein S11
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/6);  total (11/6)

AAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAAACCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTTACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGC  >  NC_000913/3441698‑3442077                                                                                                                                                                  |                                                                                                                                                                                                                           aaaaCGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAAACCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTTTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTg                                                                                                                                                                     <  1:93121/217‑1 (MQ=255)                   cgACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAAACCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTTTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGc                                                                                                                 <  1:155914/251‑1 (MQ=255)                   cgACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAAACCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTTTTGATGCCGTATTCTGTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGc                                                                                                                 <  1:98701/251‑1 (MQ=255)                                ncggACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAAACCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTTTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAAcgcg                                                                                                    >  1:263025/2‑251 (MQ=255)                                                  gAGGGATCGGAGTCACATCAGTAATGTTAGAGATGCGGAACCCTGCGGCGCTCAGAGCACGACTAGTAAATTCGCGGCCTGGACACGGCCCTTTAACCATCACTTCAAGATTTTTGATGCCGTATTATTTCACGCCGTCAGCGCAAAGATCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACAACCGGCTGTTGCCCAACCCAACGCGTTACCCTACCGATCAGTg                                                                                  >  1:231692/1‑251 (MQ=255)                                                                      gTAATGTTAGTGATGCGGAAACCTGCGGAGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAAACATAACTTCCAGATTTTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCAAACGCGTTACCCTGACGATCAGTGATAGTCACGATGgtgt                                                                  >  1:212176/1‑247 (MQ=255)                                                                                      ggAAACCGGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTTTTGATGCCGTATGCTTTCGCGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGcn                                                                                                                <  1:35004/185‑2 (MQ=255)                                                                                                   gTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCCTAACTTCCAGATTTTTGAAGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGCAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTTACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAAAAGCATGGATATGAGCCACGCCGt                                 >  1:314872/1‑251 (MQ=255)                                                                                                            aCGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGCTTTTTGATGCCGTATTCTTTAACGGCGCAAGCGCAACGCTCTGCTGCAACCTCAGCTGCAAACGGCGTGGATTTGCGAGAACCACGGCAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTTACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCa                                                    >  1:181068/1‑223 (MQ=255)                                                                                                                      gATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTTTTGATGCCGTATTCTTTCACGGCGGCAGCGCAACGCTCTGCTGTAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCTGAACCACCGGCGGTTGCCCAACCTAACGCGTTACTCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTac              <  1:169445/251‑1 (MQ=255)                                                                                                                          cgcgGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTTTTGATGCCGTATTCTTTCACGGCGTCAGCGCCACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTTACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGt          >  1:116329/1‑251 (MQ=255)                                                                                                                          cgcgGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTTTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTTACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACg           <  1:162579/250‑1 (MQ=255)                                                                                                                             ggCCTGGACCCGGACCTTTAACCATAACTTCCAGATTTTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTTACCCTGACGATc                                                                                      >  1:107358/1‑172 (MQ=255)                                                                                                                                cTGGACCCGGACCTTTAACCATAACTTCCAGATTTTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGAAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGACCAACCCAACGCGTTACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGt    >  1:135956/1‑251 (MQ=255)                                                                                                                                   gACCCGGACCTTTAACCATAACTTCCAGATTTTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCACACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTTACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGCGACTTGTTTTCTTACACGTTTACGTGc  >  1:120915/1‑250 (MQ=255)                                                                                                                                           ccTTTNAACATNANNTCCAGATTTTAGATGCCGTNTTCTTTCACGGCGTCNGCGCNACGCTntgntn                                                                                                                                                                                 >  2:131603/1‑66 (MQ=255)                                                                                                                                                  ncATAACTTCCAGATTTTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGaa                                                                                                                               >  1:79549/2‑110 (MQ=255)                                                                                                                                                                  |                                                                                                                                                                                                                           AAAACGAGGCGTTATACGCGACGTTTTTTCGGCGGACGACAACCGTTATGAGGGATCGGAGTCACATCAGTAATGTTAGTGATGCGGAAACCTGCGGCGTTCAGAGCACGAATAGTAGATTCGCGGCCTGGACCCGGACCTTTAACCATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTTACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGC  >  NC_000913/3441698‑3442077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑