Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,777	C→T	R2H (CGT→CAT)	ybgU ←	protein YbgU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,777	0	C	T	100.0%	37.3 / NA	14	R2H (CGT→CAT)	ybgU	protein YbgU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/3);  total (11/3)

CCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCAAATTAGATGATTAAAAATTAAATAAATGTTGTTATCGTGACCTGGATCACTGTTCAGGATAAAACCCGACAAACTATATGTAGGTTAATTGTAATGATTTTGTGAACAGCCTATACTGCCGCCAGGTCTCCGGAACACCCTGCAATCCCG  >  NC_000913/754534‑754981                                                                                                                                                                                                                                                    |                                                                                                                                                                                                             ccTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTATGAGAAAGAGGGGAAAACCAGGGGAACGAGCTCTGGGCGCTAGAAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTATGCATtat                                                                                                                                                                                                       >  1:142323/1‑251 (MQ=255)                           ntCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGCCGAATAAATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTACTAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTATGCATTATATGCTTTTCCTGGTAATGTTTGTaac                                                                                                                                                                             >  1:63042/2‑251 (MQ=255)                                 nctctGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTATGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACttt                                                                                                                                                                       >  1:82952/2‑251 (MQ=255)                                                     aCCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAAGTACTTAGCAATCCGAATGATTAAACTTGTCTACCACTAATAACTGTCCCGAAGGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTATGCATTATATGc                                                                                                                                                                                                   <  1:167430/203‑1 (MQ=255)                                                                                                                              tAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTATGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTttgttg                                                                                                                                                                   <  1:55441/162‑1 (MQ=255)                                                                                                                                                                                      tCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAACTACCGACTTCATAACTTATATCCATTATATGCTTTTCCTCGTAATGTTTGTacc                                                                                                                                                                             >  1:130555/1‑94 (MQ=255)                                                                                                                                                                                       cAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTATGCATTATATGCTTTTCCTGGTAATGTTTGTACCAACTTTGTTGAATGATTGTCAAATTAGATGATTAAAAATTAAATAAATGTTGTTATCGTGACCTGGATCACTGTTCAGGATAAAACCCGACAAACTATATGTAGGTTAATTGTAATGATTTTGTGAACAGCCTATACTGCCGCCAGGTCTCCGGa                  >  1:245859/1‑250 (MQ=255)                                                                                                                                                                                       cAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTATGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCAAATTAGATGATTAAAAATTAAATAAATGTTGTTATCGTGACCTGGATCACTGTTCAGGATAAAACCCGACAAACTATATGTAGGTTACTTGTAATGATTTTGTGAACAGCCTATACTGCCGCCAGGTCTCCGGa                  >  1:245847/1‑250 (MQ=255)                                                                                                                                                                                       cAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTATGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCAAATTAGATGATTAAAAATTAAATAAATGTTGTTATCGTGACCTGGATCACTGTTCAGGATAAAACCCGACAAACTATATGTAGGTTAATTGTAATGATTTTGTGAACAGCCTATACTGCCGCCAGGTCTCCg                    >  1:41435/1‑248 (MQ=255)                                                                                                                                                                                       cAATACTTCCACAATGTTACATAAGTTAATCTTAGGTGCAATACCGACTTAATAACTTTTATGCATTATCTGCTTTTCCTGGTAATGATTGTAACAACTTTGTTGCATGATTGTGCAATTAGATGATTAACAATTAAATAAATGTTGTTATCGTGACCTAGATCACTGTTAAGGATAAAACCCGACAAACTATATGTAGGTTAATTGTACTGATTTTGTGAACAGCCTATACTGCCGCCAGGTCTCCGGa                  >  1:245858/1‑250 (MQ=255)                                                                                                                                                                                                     tggtACATAAGTTAATCTTAGGTGAAATACCGACTGCATAACTTTTATGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCAAATTAGATGATTAAAAATTAAATAAATGTTGTTATCGTGACCTGGATCACTGTTCAGTATAAAACCCGACAAACTATATGTAGGTTAATTGTAATg                                                        <  1:235909/195‑1 (MQ=255)                                                                                                                                                                                                     tGTTANNTANNNTANNCTTAGGTGAAATACCGACTTCATNACTTTTATGCATTATATGCTTTTCCTGGTNANGNTTGTNNCAANTTTGTTGAATNATTGTCAAATTAGATGATTAAAAATTAAATAAATGTTGTTATCGTGACNTNGATCACTGTTCAGGATAAAACCCGACAAACTNTATGTAGGTTAATTGTAATg                                                        >  2:235909/1‑198 (MQ=255)                                                                                                                                                                                                      gTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTATGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCAAATTAGAAGATTAAAAATTAAATAAATGTTGTTATCGTGACCTGGATCACTGTTCAGGATAAAACCCGACAAACTATATGTAGGTTAATTGTAATGATTTTGTGAACAGCCTATACTGCCGCCAGGTCTCCGGAACACCCTGCAATCCCg  >  1:266975/1‑251 (MQ=255)                                                                                                                                                                                                                     cTTAGGTGAAATACCGACTTCATAACTTTTATGCATTATATGCTTTTCATGGTAATCATTGTAACAACTTTGTCGAATGATTGTCCAATTAGATGATTCAAAATTAAATAAATGTTGTTATCGTGACCTGGATCACTGTTCAGGATAAAACCCGa                                                                                   >  1:311968/1‑155 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                             CCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTCTACCACTAATAACTGTCCCGAATGAATTGGTCAATACTTCCACACTGTTACATAAGTTAATCTTAGGTGAAATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGATTGTCAAATTAGATGATTAAAAATTAAATAAATGTTGTTATCGTGACCTGGATCACTGTTCAGGATAAAACCCGACAAACTATATGTAGGTTAATTGTAATGATTTTGTGAACAGCCTATACTGCCGCCAGGTCTCCGGAACACCCTGCAATCCCG  >  NC_000913/754534‑754981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑