Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,425,291	C→T	H58H (CAC→CAT)	rpsF →	30S ribosomal subunit protein S6

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,425,291	0	C	T	100.0%	24.4 / NA	11	H58H (CAC→CAT)	rpsF	30S ribosomal subunit protein S6
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

AGGAGCAATTCGATGCGTCATTACGAAATCGTTTTTATGGTCCATCCTGACCAGAGCGAACAGGTTCCGGGCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGCCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGTTATGCGTACCAAGCACGCTGTTACCGAAGCATCTCCGATGGTTAAAGCGAAAGACGAGCGCCGTGAGCGTCGCGATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGA  >  NC_000913/4425106‑4425506                                                                                                                                                                                          |                                                                                                                                                                                                                        aGGAGCAATTCGATGCGTCATTACGAAATCGTTTTTATGGTCCATCCTGACCAGAGCGAACAGGTTCCGGGCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGCCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACATTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCtn                                                                                                                                                        <  1:201581/251‑2 (MQ=255)                                            aTCCTGACCAGAGCGAACAGGTTCCGGGCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGCCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACATTACGTTCTGATGAAGGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGTTATGCGTACAAAGCAc                                                                                                             >  1:16121/1‑251 (MQ=255)                                                                                 gcgcTACACGGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGCTGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACATTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGTTATGCGTACCAAGCACGCTGTTACCGAAGCAGCTCCGATg                                                                                     <  1:40740/238‑1 (MQ=255)                                                                                                   cACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGCCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACATTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGTTATGCGTACCAAGCACGCTGTTACCGAAGCATCTCCGATGGTTAAAGCGAAAGACGAGCGCCGTGAGCGTc                                                      >  1:71121/1‑251 (MQ=255)                                                                                                       ggTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGCCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACATTACGTTCTGATGAATGTTTAAGCTCCGCAGGAAGTGATCTATTAGCTTGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGTTATGCGTACCAAGCACGCTGTTACCg                                                                                                   <  1:246957/202‑1 (MQ=255)                                                                                                         tGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGCCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACATTACGTTCTGATGAATGTTGAAGCtac                                                                                                                                                                                               <  1:315585/108‑3 (MQ=255)                                                                                                                                         ggggCCGCCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACATTACTTTCGTATGAATGTTGAATCTCCGAAGGAAGTGATCGATGAGCGGGAAACTCCTTTCCGCTTCAACGAGGCCGTTATCCGCAGCATGGTTATGCGTCCCAATCACGTTGTTACAGAAGCATCCCCGATGGTTAAAGCGAAAGACGAGCGCCTTGAGCGTCGCGATGATTCCGAAAACGAAACCGCTGATGATGCTGa                 <  1:276279/250‑1 (MQ=255)                                                                                                                                                     aGCTGGCTTACCCGATCAACAAACTGCACAAAGCACATTACGTTCTGATGCATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTCTCCGCAGCCTGGTTCTGCGTACCAAGCCCGCTGTTACCGAAGCATCTCCGATGGTTAAAGCGAAAGACGAGCGCCGTGAGCGTcg                                                     >  1:331906/1‑202 (MQ=255)                                                                                                                                                     aGCTGGCTTACCCGATCAACAAACTGCACAAAGCACATTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGTTCTGCGTACCAAGCACGCTGTTACCGACGCCTCTCCGATGGTTAACGCGACAGACGAGCGCCGTGAGCGTcg                                                     >  1:331910/1‑202 (MQ=255)                                                                                                                                                          gCTTACCCGATCAACAAACTGCACAAAGCACATTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGTTATGCGTCCCAAGCACGCTGTTCCCGAAGCATCTCCGATGGTTAAAGCGAAAGACGAGCGCCGTGAGCGTCGCGATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGa  >  1:104669/1‑248 (MQ=255)                                                                                                                                                                             tGNACANANNANNTTANGTTCTGATGAATGTTGAAGCTNNGCAGGAAGTNATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATNGTTATGCGTNNCANGCACGNTNNNANNNAANCATCTCCGATGGTTAAAGCNAANNACGAGCNCCGTGAGCGTCGCGATGATTTCGCaa                                       <  2:71121/192‑1 (MQ=255)                                                                                                                                                                                          |                                                                                                                                                                                                                        AGGAGCAATTCGATGCGTCATTACGAAATCGTTTTTATGGTCCATCCTGACCAGAGCGAACAGGTTCCGGGCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGCCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGTGATCGATGAGCTGGAAACTACCTTCCGCTTCAACGATGCCGTTATCCGCAGCATGGTTATGCGTACCAAGCACGCTGTTACCGAAGCATCTCCGATGGTTAAAGCGAAAGACGAGCGCCGTGAGCGTCGCGATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGA  >  NC_000913/4425106‑4425506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑