Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	74,996	G→A	T162I (ACA→ATA)	thiB ←	thiamine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	74,996	0	G	A	100.0%	41.3 / NA	15	T162I (ACA→ATA)	thiB	thiamine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/12);  total (3/12)

AGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATC  >  NC_000913/74810‑75234                                                                                                                                                                                           |                                                                                                                                                                                                                                               agaATGTGATAAGCCGGAGAGGTGTTGTAACTCAGTACCAGATCTCTTTCACCTTTTAAAAACAGGCCGGAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCGAGTGTCTGCTAGGGTTGTGTGGCGTCATCTCCATAGACTTTTTGCATCCAGAGCAACAGGCTCAGCCCCTGTATACGGATGCTCGTATCCTGATAAATCACCCGCCAGTTTTGAGCGCTCTCAACCAGTTCTTTCAg                                                                                                                                                                                <  1:123793/251‑1 (MQ=255)                                               tttCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTATACTGGTGCGCGGATc                                                                                                                                                                                                                                <  1:14042/157‑1 (MQ=255)                                                                  gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTATACTGGTg                                                                                                                                                                                                                                        >  1:76256/1‑130 (MQ=255)                                                                  gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTNCTTCGCCAGNNTCNGCCAGNCNNNTNNNGCNTCAGCGCCATAGACTTTTTNCANNCATAGCNACAGACCCAGCCCCGGTATACTGGTg                                                                                                                                                                                                                                        <  2:76256/130‑1 (MQ=255)                                                                        ggCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTATACTGGTGCGCGGATCCt                                                                                                                                                                                                                              >  1:8757/1‑134 (MQ=255)                                                                              gCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTATACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTtgtg                                                                                                                                                                        <  1:247295/182‑1 (MQ=255)                                                                                     ccTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTATACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTg                                                                                                                                                       <  1:236814/192‑1 (MQ=255)                                                                                        ttGGGGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTATACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTcc                                                                                          <  1:105773/250‑1 (MQ=255)                                                                                                           tnntcGCCANNTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTATACTGGTGCGCGGATCCt                                                                                                                                                                                                                              <  2:8757/99‑1 (MQ=255)                                                                                                                                                          ttttGCAGCCATAGCAACAGACCCACGCCCTGTATACTTGTGAGCGGAGCCTGATAAAGCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGTCGTTGTTGCGGGTTTTTCATTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATGCCAGCCACCGTGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTgg                        <  1:220602/250‑1 (MQ=255)                                                                                                                                                               cATCCATAGCAACAGACCCAGCCCCGGTATATTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAg                                                                                                                                                                                <  1:8866/93‑1 (MQ=255)                                                                                                                                                               cATCCATAGCAACAGACCCAGCCCCGGTATACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTNNCAg                                                                                                                                                                                >  2:8866/1‑93 (MQ=255)                                                                                                                                                                   cATAGCAACAGACCCAGCCCCGGTATACTGTTGCGCGGATCCTGATAAAGCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGt                                                                                                                                                      <  1:275287/115‑1 (MQ=255)                                                                                                                                                                               cccAGCCCCGGTATACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGTGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACTGCATCCTCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATc  <  1:306165/251‑1 (MQ=255)                                                                                                                                                                               cccAGCCCCGGTATACTGGGTCGCGGATCCTGATAAATCGCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTc                                                                                                                                                             <  1:321084/96‑1 (MQ=255)                                                                                                                                                                                           |                                                                                                                                                                                                                                               AGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATC  >  NC_000913/74810‑75234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑