Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,582,293	C→T	G78R (GGA→AGA)	ydeN ←	putative sulfatase YdeN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,582,293	0	C	T	100.0%	44.9 / NA	16	G78R (GGA→AGA)	ydeN	putative sulfatase YdeN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/7);  total (9/7)

CCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAGCTTCAATGGCTTTATCTATCCCTATTTTGTAGGTATCGACAACTTCACGATTTTCCATTGTTTTTGGGTCAAAAGATCCCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCT‑TTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAGCAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTC  >  NC_000913/1582079‑1582532                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 ncAAAGCGGGCGGGAGCTCGAACGGTCATTATTGCGGCGCGGGAGGGGCGGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAGCTTCAATGGCTTTATCTATCCCTATTTTGTAGGTATCGACAACTTCACGATTTTCCATTGTTTTTGGGTCAAAAGATCTCTTATCAAAAGGAAGATGTCCATAACCAAGatcatc                                                                                                                                                                                                              >  1:78287/2‑251 (MQ=255)                                       gcgGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATACATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAGCTTCAATGGCTTTATCTATACCTATTTTGTAGGTATCGACAACTTCACGATTTTCCATTGTTTTTGGGTCAAAAGATCTCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCT‑TTGGTa                                                                                                                                                                        >  1:292590/1‑250 (MQ=255)                                                gCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAGCTTCAATGGCTTTATCTATCCCTATTTTGTAGGTATCGACAACTTCACGATTTTCCATTGTTTTTGGGTCAAA‑GATCTCTTATCAAAAGGAAGTTGTCCATAACCAAg                                                                                                                                                                                                                    >  1:73735/1‑197 (MQ=255)                                                     aanCACNGNNTNNCACNTAGCCGTTAGTAAAACGTACGNNTTCATCCATNAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAGCTTCAATGGCTTTATCTATNCCTATTTTGNNGGNATCGANANNNTNNNGANTTTCCATTGTTTTTGGGTCNAA‑NNTCTCTTNTCAAAAGGAAGTTGTCCATAACCAAg                                                                                                                                                                                                                    <  2:73735/192‑1 (MQ=255)                                                                               gTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAGCTTCAATGGCTTTATCTATCCCTATTTTGTAGGTATCGACAACTTCACGATTTTCCATTGTTTTTGGGTCAAAAGATCTCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCT‑TTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAGc                                                                                                                                        <  1:100338/242‑1 (MQ=255)                                                                                                   cATTAATTAAATGAGCGTCGGCGTTGATTGTTGTGCAGCTTCAATGGTTTTATCTATCCCTAGTTTGTAGGTCTCGCCAACTTCACGATTTGCCACTGTTTTTGGGTCAAAAGATCTCTCATCCAAAGGAAGTTGTCCAAAACCAAGATCATCCATGGTCAGTACGATAATATTTCGATTTCCTGTTTG‑ACTGGTTTCTGGCGGCTTAAAGCCTGAGAAAGCAACGTTTGTTTTGGTTg                                                                                                                       <  1:121369/239‑1 (MQ=255)                                                                                                    aTTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAGCTTCAATGGCTTTATCTATCCCTATTTTGTAGGTATCGACAACTTCACGATTTTCCATTGTTTTTGGGTCAAAAGATCTCTTATCAAAAGGAAGTTGtn                                                                                                                                                                                                                              <  1:84735/136‑2 (MQ=255)                                                                                                                                    tgCAGCTTCAATGGCTTTATCTATCCCTATTTTGTAGGTATCGACAACTTCCCGATTTTCCATTGTTTTTGGGTCAAAAGATCTCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCT‑TTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAGCAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGcc                                                                           >  1:187165/1‑250 (MQ=255)                                                                                                                                      cAGCTTCAATGGCTTTATCTATCCCTATTTTGTAGGTATCGACAACTTCACGATTTTCCATTGTTTTTGGGTCAAAAGATCTCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATNATANNNNNCtnnnnnnnnnn                                                                                                                                                                          >  2:203043/1‑144 (MQ=255)                                                                                                                                        gCTTCAATGGCTTGATCTATCCCTATTTTGTAGGTATCGACAACTTCACGATTTGCCATTGGTTTTGGGTCAAAAGATCTCTTATCAAAATGAAGTTGTCCATAACCAAGATCATCCATGGGCTGTACGATAATATTTGGCTTTCGT‑TTGGTACTGTAGGCTGTCGGCGTAAAGGCTGAGAAAGCAGTGTTTGTTTTGGGTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGCCATAcc                                                                      <  1:182839/251‑1 (MQ=255)                                                                                                                                                 gCTTTATCTCTCCCTATTTTGTAGGTATCGACAACTTCACGATTTTCCATTGTTTTTGGGTCAAAAGATCTCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCT‑TTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAGCAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGCCATACCAGATGcc                                                               >  1:267406/1‑249 (MQ=255)                                                                                                                                                            cccTATTTTGTAGGTATCGACAACTTCACGATTTTCCATTGTTTTTGGGTCAAAAGATCTCTTATCAAAAGGAAGTTGTCCCTAAACAAGATCATCCATGGTCAGTACGCTCATATTTGGCTTTCCT‑TTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAGCAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTCTCAAAGat                                                   >  1:244469/1‑250 (MQ=255)                                                                                                                                                                                  aCTTCACGATTTTCCATTGTTTTTGGGTCAAAAGATCTCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGCTAATATTTGGCTTTCCT‑TTGGTACTGTATTCTGTCGGCGTCAAGTCTGAGAAAGCAACGTTTGTTTTGGTTGCt                                                                                                                     >  1:21001/1‑162 (MQ=255)                                                                                                                                                                                      cACGATTTTCCATTGTTTTTGGGTCAAAAGATCTCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCT‑TTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAGCAACGTTTGTTTTGGTTGCTTTCAg                                                                                                                <  1:290602/163‑1 (MQ=255)                                                                                                                                                                                        cGATTTTCCATTGTTTTTGGGTCAAATGATCTCTTATCCAAAGGAAGTTGTCCACAACCAAAATCATCCATGGTCAGAACGATAATATTTGGCTTTCCT‑TTGGCACTGTATTCTGTCGGCGTAAAGTCTGAGAAAGCAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTa                       >  1:129659/1‑250 (MQ=255)                                                                                                                                                                                                            gTCAAAAGATCTCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCT‑TTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAGCAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGttn  <  1:203043/251‑2 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 CCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGCAGCTTCAATGGCTTTATCTATCCCTATTTTGTAGGTATCGACAACTTCACGATTTTCCATTGTTTTTGGGTCAAAAGATCCCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCT‑TTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAGCAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTC  >  NC_000913/1582079‑1582532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑