Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	757,538	C→T	D544D (GAC→GAT)	sdhA →	succinate:quinone oxidoreductase, FAD binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	757,538	0	C	T	100.0%	16.1 / NA	8	D544D (GAC→GAT)	sdhA	succinate:quinone oxidoreductase, FAD binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/2);  total (6/2)

GCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCC  >  NC_000913/757341‑757723                                                                                                                                                                                                      |                                                                                                                                                                                          gCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAACATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGTCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGATTTCCCGGATCGtgatgatga                                                                                                                                                                       >  1:115078/1‑218 (MQ=255)        cGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGTCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGATTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGcc                                                                                                                                         <  1:19640/241‑1 (MQ=255)              ggCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACACAGCGCGTTGAGTGTCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGATTTCCCGGATCGTGATGATGAAAACTGGCTGTGc                                                                                                                                                          >  1:185986/1‑218 (MQ=255)                               gTGATCCGCGAGCGTCTGACAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGTCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAACCGCTTCGATTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCa                                                                                                         >  1:267113/1‑250 (MQ=255)                                                                              cGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATACCCTGATGGAAACGGCGTCTGCAACGGCTGTTTCTGCCACCTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGATTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGCACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGtactt                                                         >  1:172484/1‑251 (MQ=255)                                                                                                    gAGTGTCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGAATAGCCGCTTCGATTTACAGGACCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCTAGAGTCGGAATCCATGACGCGCCGAAGAGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAAt                                   >  1:326765/1‑251 (MQ=255)                                                                                                                                     gCGTATGCAACGGCTGTTTCTGCCAACTTCAGTACCGAAAGCCGTGGCGCGCATACCCGCTTCGATTTCCCGGATCGTGATGATGAACACTGGCTGTGCCACTCCCTGTATCTCCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGCGAAAGGAAAATGAGCCTCGAGTTTTCAATTTATCGCTATAAccc  >  1:122784/1‑251 (MQ=255)                                                                                                                                                                 tNCGNACCGANAGCCNTGGCGCGCATAGCCGNTTCGATTTTCCGGATCGTGNNGNTGAAANCTGGc                                                                                                                                                               <  2:121538/66‑1 (MQ=255)                                                                                                                                                                                                      |                                                                                                                                                                                          GCGATGGCGAAAGGGCTTGAGCAGTTGAAAGTGATCCGCGAGCGTCTGAAAAATGCCCGTCTGGATGACACTTCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCC  >  NC_000913/757341‑757723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑