Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,299	C→T	intergenic (‑117/‑53)	yoaI ← / → yeaL	protein YoaI/DUF441 domain‑containing inner membrane protein YeaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,299	0	C	T	100.0%	34.0 / NA	14	intergenic (‑117/‑53)	yoaI/yeaL	protein YoaI/DUF441 domain‑containing inner membrane protein YeaL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/8);  total (6/8)

GCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCTTTTTTCCGTGGATTGAAAAACAGGGGCTAAGTATCGGGATAATCATTCTGACGATTGGCG  >  NC_000913/1874072‑1874535                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             gCGTAACTAGCCTGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAAGCAGTGTCTCGACAAACATGTGATCGGTCATAGCATGCCCCCGGGAGAGAGCTGTGGTCACGTTATTGTTAGCGTGCACAAATTGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTATCGACGTCCTTCTTGCAGCAAGCGTAAAGTAAGc                                                                                                                                                                                                                         <  1:151534/249‑1 (MQ=255)         aGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCTTGCAGCAAg                                                                                                                                                                                                                                     <  1:189302/229‑1 (MQ=255)                                        aaCCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCTCGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTGTCTACTTCCTTCTTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATg                                                                                                                                                                                <  1:225782/251‑1 (MQ=255)                                                      cAATGGCAAAAAACGATGACGTGAGAATCAGTGTCTCGACAAACATTTGATCGATCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCTCAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTGCTTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTGTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGAtn                                                                                                                                                                  <  1:57136/251‑2 (MQ=255)                                                            cAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCTTGCAGCAAGCGTAAAGTAAGCAGGCTTATTAtttt                                                                                                                                                                                                           >  1:298238/1‑204 (MQ=255)                                                                                                                                           cACGTTATTGTTAGCGTGCAAAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCTTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGtn                                                                              <  1:194854/250‑2 (MQ=255)                                                                                                                                                             cacaAATGGCCGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTACAGATTTTCTACTTCCTTCTTGCAGCAAGCGTAAAGTAAGCCGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCTATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCtttt                                                           >  1:111737/1‑251 (MQ=255)                                                                                                                                                                                           naTTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCTTGCAGCAAACGTAAAGTAAGCAGGCTTCTTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTTGCTGCGCTGGGCTTTATCAGTCa                                                                                                                               >  1:191868/2‑153 (MQ=255)                                                                                                                                                                                                  nntGNNNNNAACNTAAAGATTTTCTACTTCCTTCTTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCa                                                                                                                               <  2:191868/144‑1 (MQ=255)                                                                                                                                                                                                      cAAAAAACGTAAAGATTTTCTACTTCCTTCTTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAAt                                                                                           >  1:42188/1‑178 (MQ=255)                                                                                                                                                                                                      cAAAAAACGTAAAGATTTTCTACTTCCTTCTTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGATGATCCTGCTCGGGTTCGCTgcg                                                                                                                                                >  1:289949/1‑125 (MQ=255)                                                                                                                                                                                                              gTAAAGATTTTCTACTTCCTTCTTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTCGCAAGGAAACCAAGATGTTTGATGTCCCTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCTTTTTTCCGTGGATTGAAAAACAGGGGCTAAGTATCGGGATAATCATTCTGAc          >  1:177733/1‑251 (MQ=255)                                                                                                                                                                                                                   gATTTTCTACTTCCTTCTTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTGTAGCAGTCATAAcaccac                                                                                                                      <  1:88758/138‑1 (MQ=255)                                                                                                                                                                                                                       ttCTACTTCCTTCTTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCTTTTTTCCGTGGATTGAAAAACAGGGGCTAAGTATCGGGATAATCATTCTGACGATTGGCg  <  1:119731/250‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             GCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCTTTTTTCCGTGGATTGAAAAACAGGGGCTAAGTATCGGGATAATCATTCTGACGATTGGCG  >  NC_000913/1874072‑1874535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑