Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,789,682	C→T	P24S (CCT→TCT)	ydiE →	PF10636 family protein YdiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,789,682	0	C	T	100.0%	35.7 / NA	13	P24S (CCT→TCT)	ydiE	PF10636 family protein YdiE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/8);  total (5/8)

CCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAGCCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGC  >  NC_000913/1789490‑1789895                                                                                                                                                                                                 |                                                                                                                                                                                                                      ccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGTCAATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCg                                                                                                                                                              >  1:75267/1‑250 (MQ=255)                              cAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTTAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGTCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTcc                                                                                                                                                                                  <  1:314167/201‑1 (MQ=255)                              cAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGTCTATTCGGCGAATTTCCAGCCAGACACTGTTAgg                                                                                                                                                                                     <  1:161353/198‑1 (MQ=255)                               naCTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATCTTGATACTAAGAATCCTTGTTATATAACTTCTTCTTAATTTTTCTGCGTTCTAAGGATAGCAGAAACATCACGCCTGAAACGGATGCAAATCACAAGCCAGCTTCCCCGCAATCTATTCGGCGAATTTCAAGCCAGACAATGTTAGATCAGGATGGCAAACTGATAATCGATCATGACGGGCAAGAATATCTGCTCCGTa                                                                                                                                >  1:37950/2‑250 (MQ=255)                                           tctcAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGTCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGc                                                                                                                      <  1:235752/248‑1 (MQ=255)                                           tctcAACAAATTACTGCTTACTCTGGTCAGACATAATATTGATAATTAGAATCCTTGTTATATCTATTATTATTAATTTTTATGCGTTAGACGGTTAGCAGAAAACTCACGACTGAAACGGATTCCAATCACAAGACCGCTTCCCCGCAGTCTATTCGGCGAATTTCCAGCCAGACTCTGTTAGGTTCGGATGGCAAACTGATTATCGATCATGACGAGCAAGAATATCTGCTCCGTAAATCTCAGGc                                                                                                                      <  1:235760/248‑1 (MQ=255)                                                      tACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTGCCCCGCAGTCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGTCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAgctgctn                                                                                                         <  1:61376/250‑2 (MQ=255)                                                       aCTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGTCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGt                                                                                                        <  1:280165/250‑1 (MQ=255)                                                               ctctGGTCAGCCATAATATTGATAATAAGAATCATTGGTAACTCAATTATTATTACTTTTTATGACTTCTACGGATAGCAGAACACTAACGCCTGAAACGGATGCCAATAACAAGACCGCTTCCCCGCAGTCTCTTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGAACATGAAGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCacgt                                                                                               >  1:220318/1‑248 (MQ=255)                                                                         ncATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGTCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAGCCTTTAAc                                                                                     >  1:83201/2‑251 (MQ=255)                                                                           atnataNTNNTNNTAANAATCATTGTTATATCAATTATNNTTAATTTTTNTGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGNCCGCTGCCCNNCANTCAATNCNNNGNNNTTNCAGCCAGACACTGTTAGGTNCGNNGGGCAANCTGATTATCGATCATGACGGGCAAGa                                                                                                                                              <  2:75267/192‑1 (MQ=255)                                                                           ataataTTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGTCTATTCGGCGAATTGCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGTCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAGCCTTTAACTc                                                                                   <  1:146497/251‑1 (MQ=255)                                                                                                                                                            tGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGTCTATTCGGCGCCTTTCCAGCCAGACACTGTTAGGTCCGGATGGCAACCTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAGCCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGc  >  1:178515/1‑251 (MQ=255)                                                                                                                                                                                                 |                                                                                                                                                                                                                      CCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGCAAGAATATCTGCTCCGTAAAACTCAGGCTGGCAAGCTGCTGTTGACCAAGTAGCCTTTAACTCGAGCAGCTGACTTCCAGCCGTTTACCCCAGTCAGGTGGACGACTGACATAGTCATCATCTCTGTCGCTGAAAGGATTTCGC  >  NC_000913/1789490‑1789895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑