Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,346,924	G→A	intergenic (+24/+54)	ymiB → / ← rnb	putative protein YmiB/RNase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,346,924	0	G	A	100.0%	10.6 / NA	6	intergenic (+24/+54)	ymiB/rnb	putative protein YmiB/RNase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/3);  total (3/3)

TGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTC  >  NC_000913/1346686‑1347049                                                                                                                                                                                                                                               |                                                                                                                              tGGATTGTGCGATTCGATTAAGTTGTTCAACGTTGTGGACTCCCTAACGGTTTTCATGATGCAGATTCTCCGGTTAACAGCGGAGTACGCGGTTAAATAAAAGGAACAACATGACTTACATGAAAGTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGGAATCCAGTTTAGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCAGCCTGGATATAACGAAAGTAGAGCgggg                                                                                                                    <  1:120732/250‑1 (MQ=255)   gATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAAAGAAAGTAGAGCGGGGaa                                                                                                                  >  1:90977/1‑250 (MQ=255)                        tGTACAACGGTGTGGACTCCCTAACGGTTTTCGTGATGCATAGCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATGAACGGCAGCTAAACGCATTGTTGTTTTGAGATAGTTATTTGGAATCCAGTTTTGGAAAAACTCCATTTTTCAATCGAAAGTCAGTTAAAAAGTCGGCCTGGATATAGCGAAAGTAGAGCGGGGAAATAAACGGCCCATCCATGAgg                                                                                             <  1:221432/250‑1 (MQ=255)                                          cccTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAAAAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTTTAATCCAGTTTTGGCAAAAAGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATATGCCTGGATATAAAGAAAGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGGATGGGCCGGGAAAgg                                                                             >  1:314604/1‑248 (MQ=255)                                                                                                  taaaAGGAACACCACGACTTACATGAAATTAACGGAGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGATTTGGAAAAACGCCAGTTTTCAAACGAAAGTAAGTTAAAAAATCTGCCTGGATATAACGAAAGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGAACGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATa                   >  1:117293/1‑250 (MQ=255)                                                                                                                     tACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAAGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCTTGAAAGGAGATTACGTTACCAGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACgtc  <  1:134266/248‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                              TGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAAAAAATCTGCCTGGATATAACGAAGGTAGAGCGGGGAAATAAACGGCCCATCCATGAGGAATGGGCCGTGAAAGGAGATTACGCGACCGGGCGCGCAATAATGCTGCGGGTTTCCATGCGGACTTCGGCAATAGTGACGTCAATAACGTC  >  NC_000913/1346686‑1347049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑