Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	383,799	G→A	A46V (GCG→GTG)	yaiP ←	putative glucosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	383,799	0	G	A	100.0%	30.1 / NA	13	A46V (GCG→GTG)	yaiP	putative glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/7);  total (6/7)

GAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCA  >  NC_000913/383569‑384042                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gAACATAGGTGTCTGCATCACTTAAAAAAACCTGGTCGCATGTGGCGTCATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCATTTACTGCACCAGACACGGGCCTTCGtt                                                                                                                                                                                                                                  >  1:306669/1‑250 (MQ=255)                                   gTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCACCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACGGCctttct                                                                                                                                                                                               >  1:271265/1‑251 (MQ=255)                                                                  aGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGGGGAGGCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCACCAg                                                                                                                                                                                                                                                 <  1:44200/170‑1 (MQ=255)                                                                                                         acaaAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAACATAAGGGTTACGCAGTAGATTATCCAGTGACTGCACCAGACACGGGCCTTCGTtata                                                                                                                                                                                                                               >  1:121397/1‑149 (MQ=255)                                                                                                                    tccccATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCAGTCGTTAACGCCAATTACCCGGCAAAAATAAGGGTTACGCAGTCGATTATCCAGTGACTGCACCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTT‑AACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCAt                                                                                                             >  1:175589/2‑251 (MQ=255)                                                                                                                            tGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCTTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCACCAGTCACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACg                                                                                                                                                                               <  1:105444/178‑1 (MQ=255)                                                                                                                                        ccGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCACCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACATATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATc                                                                                          <  1:268174/251‑1 (MQ=255)                                                                                                                                                      ccgatcgtGTCCGTGGAGCCTTCGTTAACGCAAATTACCCGGCAAAAAGAAGGGTTACGCAGTAGATTATCCAGTGACTGCACCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACGTAAAATCCATAGCAACATTCCTATGCACATACATATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTAc                                                                            <  1:219947/244‑1 (MQ=255)                                                                                                                                                                                   gCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCACCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCAtt                                                                                       >  1:11199/1‑211 (MQ=255)                                                                                                                                                                                            ccGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCACCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCcgc                                      >  1:141209/1‑251 (MQ=255)                                                                                                                                                                                              ggNAAAAANAAGGNTTACGCAGTAGATTANCCAGTGACAGCACCAGACANNGNCCTTCNTtata                                                                                                                                                                                                                               <  2:121397/64‑1 (MQ=255)                                                                                                                                                                                                                       aTTATCCAGTGACTGCACCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTccn                                       <  1:96510/223‑2 (MQ=255)                                                                                                                                                                                                                                 gACTGCACCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATAGCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAAGTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGAGCATGACGCa  <  1:98874/250‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCGTTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATGAATTACAAAGCCTTCACCCAGATCGCTATTGACCTGTTGTCCGCGAAATTGTGCAACTGCACGCAGGCGATCATGACGCA  >  NC_000913/383569‑384042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑