Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,515,819	T→C	T672A (ACG→GCG)	pdeA ←	putative c‑di‑GMP phosphodiesterase PdeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,515,819	0	T	C	100.0%	22.8 / NA	10	T672A (ACG→GCG)	pdeA	putative c‑di‑GMP phosphodiesterase PdeA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/5);  total (5/5)

ATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGCCATCAATTTTGATGATATCAGCCTGCAAACGCTTTAACCGTTCGTAGTTGGCATATCCGGTGCCAAAGTCATCAATCGCAATCCGGAAACCAAACTTATGCAGCTGCTCGATGTTGTACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATG  >  NC_000913/2515612‑2516012                                                                                                                                                                                                                |                                                                                                                                                                                                  aTGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAAGCATCGCATCCAGCGTGTTCGCGACAATATCTTTCACAAAGACGCCATCAATTTTGATGATATc                                                                                                                                                         <  1:293936/250‑1 (MQ=255)                                                          cAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCTACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAAGTGATCTCACAATCATCGCATCCAGCGTGTTCGCGACAATATCTTTCACAAAGACGCCATCAATATTGATGATATCAGCAGGCAAACGCTTTAACCGTTCGTAGTTTGCATATCCGGTGCCAAAGTCATCAATc                                                                                               <  1:278865/251‑1 (MQ=255)                                                                   aCCCTTGCAGATATTGCACCCCGAGCTTATGCGATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGCGACAATATCTTTCACAAAGACGCCATCAATTTTGATTATATCATGCTGCATACGCGGTATCCGTTCGTAGTTGGCATATCCGGTGCCAAAGTCATCAATCGCAATCCg                                                                                       <  1:209293/250‑1 (MQ=255)                                                                                     ccccGAGCTTATGCAATAGAGCCTGCTGCTGTTGCGACTCGACAAACTCCGCGACCAGACTCACTGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATACAGCGTGTTCGCGACAATATCTTTCACAAAGACGCCATCAATTTTGATGATATCAGCCTGCAAACGCTTTAACAGTTCGTAGTTGGCATATCCGGTGCCAAAGTCATCAATCGCAATCCGGAAACCAAACTTATGCAgc                                                                    >  1:106601/1‑251 (MQ=255)                                                                                           ncTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGCGACAATATCTTTCACAAAGACGCCATCACTTTTGATGATATCAGCCTGCAAACGCTTTAACCGTTCGTAGTTGGCATATCCGGTGCCAAAGTCATCAATCGCAATCCGGAAACCAAACTTATGCAGCTGCTCg                                                              >  1:195486/2‑251 (MQ=255)                                                                                                            tgctgctgTTGCGTCTCGACAAACTCCGCGACCACACACAATGACTTCGCTTTCGACAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGCGACAACAGCTTTCACAAAGACGCCATCAATTTTGATGATATCAGcc                                                                                                                                                     <  1:148359/147‑1 (MQ=255)                                                                                                                           tcGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGCGACCATATCTTTCACCAAGACGCCATCAATTTTAATGATATCAGCCTGCAAACGCTTTAACCGTTCGTAGTTGGCATATCCGGTGCCACAGTCATCAATCGCAATCCgg                                                                                      >  1:169061/1‑195 (MQ=255)                                                                                                                                       ncgACCACACTCAATGACTTCGCTTTCGCCAGATCGGCAATTGATCTCCCAATCATCGCATCCAGCGTGATCCCGACCATATCTTTCAAAAAGACGCCATCAATTTTGATGATATCAGCCTGCAAACGCTTTAACCGTTCGTAGTTGGCATATCCGGTGCCAAAGTCATCAATCGCAATCCGGAAACCAAACTTATGCAGCTGCTCGATGTTGTACATGCTGCTTTCTGCATTAGAAAACGCCTGctcctc                  >  1:35759/2‑251 (MQ=255)                                                                                                                                                  naaTGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGCGACAATATCTTTCACAAAGACGCCATCAATTTTGATGATATCAGCCTGCAAACGCTTTAACCGTTCGTAGTTGGCATAACCGGTGCCAAAGt                                                                                                       >  1:94964/2‑155 (MQ=255)                                                                                                                                                       aCTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGCGACAATATCGTTCACAAAGACGCCATCAATTTTGATGATATCAGCCGGCAAACGCTTTAACCGTGCGTAGTTGGCATATCCGGTGCCAAAGTCAGCAATCGCAATCCGGAAACCAAACTGATGCAGCTGCGCGATGTTGTACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATg  <  1:315051/251‑1 (MQ=255)                                                                                                                                                                                                                |                                                                                                                                                                                                  ATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGCCATCAATTTTGATGATATCAGCCTGCAAACGCTTTAACCGTTCGTAGTTGGCATATCCGGTGCCAAAGTCATCAATCGCAATCCGGAAACCAAACTTATGCAGCTGCTCGATGTTGTACATGCTGCTTTCTGCGTTAGAAAACGCCTGCTCCTCGGTGATCTCAAGAATG  >  NC_000913/2515612‑2516012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑