Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,338,118	G→A	intergenic (‑189/+136)	adiY ← / ← adiA	DNA‑binding transcriptional activator AdiY/arginine decarboxylase, degradative

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,338,118	0	G	A	100.0%	64.7 / NA	23	intergenic (‑189/+136)	adiY/adiA	DNA‑binding transcriptional activator AdiY/arginine decarboxylase, degradative
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/13);  total (10/13)

CGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGC‑GCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTACGCTTTCACGCACATAACGTGGTAAATACCGTCAATAATTTCAGTCCCTTCAGTTTCGTGTTCAAATCCAGGGAAAT  >  NC_000913/4337914‑4338333                                                                                                                                                                                                             |                                                                                                                                                                                                                         ngCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAAC‑GCGGGGGGGGGc                                                                                                                                                                                                            >  1:195723/2‑218 (MQ=255) cGCTGCAAATCCTCATACAGGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGTGTATATAATTGTAGGATGAATATAATCATGCATAAAAATAACGAGTGTGACTAAGAGTACAAATTTTTATAGTAATTTCTCATGGACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAAC‑GCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTgcagca                                                                                                                                                                           <  1:175779/251‑1 (MQ=255)  gCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGAAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAACAAAC‑GCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTgcagca                                                                                                                                                                           >  1:209028/1‑250 (MQ=255)               aTACATGTACTCCGGAGTGCGAATAAAAGACCAGTTCCCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAAC‑GCGGGGTGGGGCCGCGCAAAAAGTATGACAtttttn                                                                                                                                                                                    <  1:262516/228‑2 (MQ=255)                             gAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCGAAGCGATACCGGGAAATGGTTATATAATTGTATGCTGAATATAAACATGCATAAAAATAAAGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAAC‑GCGGGGTGGGGCCGCGCAACAAGTCTGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCa                                                                                                                                                >  1:315650/1‑250 (MQ=255)                                                nttCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGACTATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTATCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAAC‑GCGGGGTGGGGCCGCGCAAAAAGTATGACATTtttgtttg                                                                                                                                                                                >  1:31709/2‑199 (MQ=255)                                                             caaagcGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCCTAACAATAACGCGTTTTACTAATAGTACCAATTTTTATAGTCATTTCTCATGTACATATTTTTTTACAGGATAAATCACAGGCAAAAAAAAAC‑GCGGGGTGGGGCCGCGCAACAAGTATGACATTTTTGTTTGCAGCAAttttt                                                                                                                                                                     >  1:308323/1‑197 (MQ=255)                                                                     nnnnnnaannNNNTTANATAATTATATAAGTAATATAACCATGCATAAAAATAACAAGTTTCACTAATAGTACAAATTTTTAGAGTAATTTCTCATGTACATATTTTTTTACCGGATAAATAACAGGCAAAAAAAAAC‑GCGGGGTGGGGc                                                                                                                                                                                                            <  2:195723/143‑1 (MQ=255)                                                                           nnaaNNNNNATANAATTGTATGATGAATATAAACACGCATAAAAAGAACGAGTTTTACTAATAGTACAAATTTTTACAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATACAAGGCAAAAAAAAAC‑GCGGGGTGGGGCcg                                                                                                                                                                                                          <  2:201787/144‑1 (MQ=255)                                                                              aTGGATATATAATTGTATGATGAAGATAAACAAGAAGAAAAATAACGAGTTTTACTAAAATTACAAATTTTTAAAGTAATTTCTCATGGACATATTTTTTGACAGGATAAATAACAGGAAAAAAAAAAC‑GCGGGCTGGGGCCGCGCAAAAAGTATGACAGTTTTGTTTGCAGCAATTTTTATGAAAACGt                                                                                                                                                           <  1:129890/190‑1 (MQ=255)                                                                                   tatataATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGAAAAATAACAGTCAAAAAAAAAC‑GCGGGGTGGGGCCGCGCAAAAAGTATGACAGTGTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCgg                                                                                          <  1:171803/250‑1 (MQ=255)                                                                                            gTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAAC‑GCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTggn                                                                                 <  1:204961/250‑2 (MQ=255)                                                                                                natgaATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGCTAAATAACAGGCAAAAAAAAAC‑GCGGGGTGGGGCCGCGCCAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTAACGc                                                                            >  1:191843/2‑251 (MQ=255)                                                                                                                            gAGTTTTACTAATAGTACAAATTTTTATATTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAAC‑GCTGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTGATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCTTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTTGTTACGCTTTCACGCACATAACGTGGTAAATACCg                                                <  1:293110/251‑1 (MQ=255)                                                                                                                            gAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAAC‑GCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCACTTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTACGCTTTCACGCACATAACGTGGTAAATACCg                                                >  1:164684/1‑251 (MQ=255)                                                                                                                                tttACTAATAGTACAAATTTTTATAGTAATTTCTCAGGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAAC‑GCGGGTTGGGTCCGCGCAAAAAGTATGACATTTTTGGTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGgcggcg                                                                                                                          <  1:309522/173‑1 (MQ=255)                                                                                                                                                                       aCATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAAC‑GCGGGGTGGGTCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGTCGTAATGTTATTTAAACAATTACGCCTTCAg                                                                                             <  1:277694/163‑1 (MQ=255)                                                                                                                                                                        cATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAAAAGCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGAAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTAAGCGGAATAGTGGTTACGCTTTCACGCACATAACGTGGTAAATAACGTCAATAATTTCAGTCCCTTCAGTTTCGCGTTCAAATCCAggg      >  1:219497/1‑250 (MQ=255)                                                                                                                                                                           atTTTTTTACAGGATAAATAACAGGCAAAAAAAAAC‑GCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAAGTACGCCTTCAGCGGAATAGTGGTTACGCTTTCACGCACATAACGTGGTAAATACCGTCAATAATTTCAGTCCCTTCATTTTCGTGTTCAAATCCAGGGAAAt  <  1:143323/250‑1 (MQ=255)                                                                                                                                                                                             taaCAGGCAACAAAAAAC‑GCGGGGAGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACNCCTNNNNNgg                                                                                          >  2:191858/1‑144 (MQ=255)                                                                                                                                                                                             taaCAGGCAAAAAAAAAC‑GCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTACGCTTTCACGCACATAACGTGGTAAATACCGTCAATAATTTCAGTCCCTTCAGTTTcn                     <  1:191858/213‑2 (MQ=255)                                                                                                                                                                                                cAGGCAAAAAAAAAC‑GCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTACGCTGTCACGCACATAACGTgn                                                         <  1:262582/174‑2 (MQ=255)                                                                                                                                                                                                   gCAAAAAAAAAC‑GCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTACGCTTTCACGCACATAACGTgg                                                         >  1:9243/1‑171 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                                                         CGCTGCAAATCCTCATACATGTACTCCTGAGTGCGAATAAAAGACCAGTTCTCGCTAAAGCAAAGCGATACCGGGAAATGGTTATATAATTGTATGATGAATATAAACATGCATAAAAATAACGAGTTTTACTAATAGTACAAATTTTTATAGTAATTTCTCATGTACATATTTTTTTACAGGATAAATAACAGGCAAAAAAAAGC‑GCGGGGTGGGGCCGCGCAAAAAGTATGACATTTTTGTTTGCAGCAATTTTTATGAAAACGTTGCCATACTCAAAAGGCCTAAGGCCAGGCGGCGTAATGTTATTTAAACAATTACGCCTTCAGCGGAATAGTGGTTACGCTTTCACGCACATAACGTGGTAAATACCGTCAATAATTTCAGTCCCTTCAGTTTCGTGTTCAAATCCAGGGAAAT  >  NC_000913/4337914‑4338333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑