Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	631,633	G→A	W580* (TGG→TGA)	cstA →	pyruvate transporter CstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	631,633	0	G	A	100.0%	23.5 / NA	10	W580* (TGG→TGA)	cstA	pyruvate transporter CstA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/6);  total (4/6)

CCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAAC  >  NC_000913/631447‑631819                                                                                                                                                                                           |                                                                                                                                                                                           ccTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGgctgc                                                                                                                                                                >  1:114463/1‑215 (MQ=255)               cgcTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAAGGCGTGGTCGAACCGCTGGGCGGCATTAACAATCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGACGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCg                                                                                                              >  1:146249/1‑251 (MQ=255)                       gTACTGGCGTGGGGCTACTGCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACTCTCTGTGGCCGCTGTTTGGTATTGCCAACCATATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGGGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCgg                                                                                                      <  1:230439/251‑1 (MQ=255)                                                                   gCTGGGCGGCATTCACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCCGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGACTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTg                                                                                                                                                  >  1:208656/1‑163 (MQ=255)                                                                            cATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAgg                                                                               <  1:92286/221‑2 (MQ=255)                                                                                   acTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCNGATNNNNNccnnnnnn                                                                                                                                               >  2:191814/1‑144 (MQ=255)                                                                                   acTCTGTGGCCGCTGGTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTGCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGttn                                                                             <  1:191814/216‑2 (MQ=255)                                                                                                      gTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGGCTGAGTGGCTCTGGTACCAACGGCCTGGCGGCTGATGTGTTCCCTGACCGCAGGCTGGCAGAAAGGGTGTAGCGCTGGTGCGAAAGTCTGCTTCCTGGCCAGTTCTAATAAGTTCCGGGGAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCtn                       <  1:34481/251‑2 (MQ=255)                                                                                                              aaCCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAAcn               <  1:196456/251‑2 (MQ=255)                                                                                                                                                                       aaaCGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCaacaac  <  1:114680/207‑1 (MQ=255)                                                                                                                                                                                           |                                                                                                                                                                                           CCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAAC  >  NC_000913/631447‑631819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑