Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	344,389	G→A	A72T (GCT→ACT)	yahL →	uncharacterized protein YahL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	344,389	0	G	A	92.3%	24.3 / NA	13	A72T (GCT→ACT)	yahL	uncharacterized protein YahL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (3/9);  minor base C (1/0);  total (4/9)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

AAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGATGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTATCCTTATGATTGTCGTGATGATTTAGTCGATGACGACAAACTCATTCATCTCATGGCTGCCGTACGAGACTGTGAGTGGTCAGACGATAACGCACTCACCATAAATGTGCAGTTTAATGATTTCCCTGGATTTTATGACTGGATGGATTACCCTGATCACCCGGTTAAGTTTGTTTTTCACATACTTGAAAATCAAAAAGGGACAGTTTGGGTATATGACCAGGATGATG  >  NC_000913/344163‑344563                                                                                                                                                                                                                                   |                                                                                                                                                                               aaGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGATGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTATCCTTATGATTGTCGTGATGATTTAGTCGATGACGACAAACTCATTCATCTCATGACTGCCGTACGAGACTGTGAGTgg                                                                                                                                                         <  1:228084/250‑1 (MQ=255)                                    acaATAATTTGATGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACTATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATAAATTCAAGGTTTTGATTAAACAATATTATGAGTATCCTTATGATGGTCGAGATGATTTAGTCGATGACGACAAACTCATTCATCTCATGACGTCCTTTCGAGACTGTGAGTGGTCAGACGATAACGCACTCACCATAAATGTGCAGttt                                                                                                                     <  1:172379/251‑1 (MQ=255)                                                         cGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTATCCTTATGATTGTCGTGATGATTTAGTCGATGACGACAAACTCATTCATCTCATGACTGCCGTACGAGACTGTGAGTGGTCAGAc                                                                                                                                                   <  1:122436/200‑1 (MQ=255)                                                              caaAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTATCCTTATGATTGGCGTGATGATTTAGTCGATGACGACAAACTCATTCATCTCATGACTGCCGTACGAGACTGTGAGTGGTCAGACGATAACGCACTCACGATAAATGTGCAGTTTAATGATTTCCCTGGATTTTATGACtg                                                                                           <  1:151069/251‑1 (MQ=255)                                                                                  tAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTATCCTTATGATTGTCGTGATGATTTAGTCGATGACGACAAACTCATTCATCTCATGACTGCCGTACGAGACTGTGAGTGGTCAGACGATAACGCACTCACCATAAATGTGCAGTTTAATGATTTCCCTGGATTTTATGACTGGATGGATTACCCTGATCan                                                                        <  1:60938/250‑2 (MQ=255)                                                                                                    agagGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAATGTTTTGATTAAACAATATTATGATTATCCTTATGATTGTCGTGATGATTTAGTCGATGACGACAAGCTCATTCATCTCATGACTGCCGTACGAGACTGTTAGTGGTCAGACGATAACGCTCGCACCATAAATGTGCAGGTTTAGGATTTCCCTGGATTTTATGACTTGATGGATTACCCTGATCACCCGGTTAAGTTTGTTTTTc                                                     <  1:240893/251‑1 (MQ=255)                                                                                                            tCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTATCCTTATGATTGTCGTGATGATTTAGTCGATGACGACAAACTCATTCATCTCATGACTGCCGTACGAGACTGTGAGTGGTCAGACGATAACGCACTCACCATAAATGTGCAGTTTAATGATTTCCCTGGATTTTATGACTGGATGGATTACCCTGa                                                                            <  1:190503/220‑1 (MQ=255)                                                                                                                          ntttCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTATCCTTATGATTGTAGTGATGATTTAGTCGATGACGACAAACTCATTCATCTCATGACTGCCGTACGAGACTGTGAGTGGTCAGACGATaa                                                                                                                                              >  1:39690/2‑140 (MQ=255)                                                                                                                              ccTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTATCCTTATGATTGTCGTGATGATTTAGTCGATGACGACAAACTCATTCATCTCATGACTGCCGTACGAGACTGTGAGTGGTCAGACGATAACGCACTCACCATAAATGTGCAGTTTAATGATTTCCCTGGATTTTAGGACTGGAGGGATTACCCTGTTCACCCGGTTAAGTTTGTTTTTCACATACTTGAAGATCAAAAAGGGan                            <  1:262177/250‑2 (MQ=255)                                                                                                                                                 ggTTTTGATTAAACAATATTATGATTATCCTTATGATTGTCGTGATGATTTAGTCGATGACGACAAACTCATTCATCTCATGACTGCCGTACGAGACTGTGAGTGGTCAGACGATAACGCACTCACCATAAATGTGCAGTTTAATGATTTCCCTGGATTTTATGACTGGATGGATTACCCTGATCACCCGGTTAAgt                                                              >  1:73446/1‑197 (MQ=255)                                                                                                                                                      tGNTTANANNANNTTANGATTATCCTTATGATTGTCGTNNTGATTTAGTNGATGACGACAAACTCATTCATCTCATGACTGCCGTACGAGACTGTGAGTGGTCNGACGATAACNNACNCACCANANNNGNNNAGNTTAATGATTTCCCTGGATTNTANNACTGGANGGATTACCCTGATCACCCGGTTAAgt                                                              <  2:73446/192‑1 (MQ=255)                                                                                                                                                       gATTAAACAATATTATGATTATCCTTATGATTGTCGTGATGATTTAGTCGATGACGACAAACTCATTCATCTCATGCCTGCCGTACGAGACTGTGAGTGGTCAGACGATAACGCACTCACCATAAATGTGCAGTTTAATGATTTCCCTGGATTTTATGACTGGATGGATTACCCTGATCACCCGGTTAAGTTTGTTTTTCACATACTTGAAAATCAAAACGGGACAGTTTGGGTATATGACCAGGatgatg  >  1:114376/1‑251 (MQ=255)                                                                                                                                                                                                                cAAACTCATTCATCTCATGACTGCCGTACGAGACTGTGAGTGGTCAGACGATAACGCACTCACCATAAATGTGCAGTTTAATg                                                                                                                 >  1:108445/1‑83 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                               AAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGATGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTATCCTTATGATTGTCGTGATGATTTAGTCGATGACGACAAACTCATTCATCTCATGGCTGCCGTACGAGACTGTGAGTGGTCAGACGATAACGCACTCACCATAAATGTGCAGTTTAATGATTTCCCTGGATTTTATGACTGGATGGATTACCCTGATCACCCGGTTAAGTTTGTTTTTCACATACTTGAAAATCAAAAAGGGACAGTTTGGGTATATGACCAGGATGATG  >  NC_000913/344163‑344563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑