Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	286,109	G→A	A239T (GCG→ACG)	yagG →	putative D‑xylonate transporter YagG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	286,109	0	G	A	100.0%	37.0 / NA	14	A239T (GCG→ACG)	yagG	putative D‑xylonate transporter YagG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/9);  total (5/9)

CATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATACGTGATGGATCACCCGGAGTTGGCGACCCAGTTTTTACTTTACGGCAGCCTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCACATCGCGC  >  NC_000913/285904‑286349                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 catcGGCAAAGGGGACGAGCAGGAGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCACGATGATGACGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCg                                                                                                                                                                                                     >  1:212379/1‑251 (MQ=255)                                 tCGGCGCCATGTGCGTGCTGGGGCTGGGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGACGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATACGTGATGGATCACCCCGAGTTGGc                                                                                                                                                                      <  1:276256/250‑1 (MQ=255)                                 tCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGACGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATACGTGATGGATCACCCGGAGTTGGc                                                                                                                                                                      <  1:177525/250‑1 (MQ=255)                                                                      gctgctCTACGTCTGCTGCTTCACGACCAAAGAGCGCTACACCTCTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGACGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCTTGAAATACGTGATGTATGACCCGGAGTTGTCGACCCAGTTTTTACTTTACGGCAGCCTCGCCACCATg                                                                                                                                 <  1:158580/250‑1 (MQ=255)                                                                                                 cAAAGAGCGCTACTCCTGTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGAGGACGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATACGTGATGGAGCACCCGGAGTTGTCGTCCCAGTTtttacttg                                                                                                                                                     <  1:91302/203‑2 (MQ=255)                                                                                                               ccTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGACGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGACATACGTGATGGATCACCCGGAGTTGGCGACCCAGTTTTTACTTTACGGCAGCCTCGcc                                                                                                                                       >  1:182662/1‑203 (MQ=255)                                                                                                                               gggCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGACGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATACGTGATGGATCACCCGGAGTTGGCGACCCAGTTTTTACTTTACGGCAGCCTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTc                                                                        >  1:132346/1‑250 (MQ=255)                                                                                                                                 gttcgtcgGTGACGCAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGAGCATCATGTGCGCGTTCAAGATGATGACGACCTGCTCCAACGTGGTGCGAGGCGGGGCGACGCTCTACTTCGTGAAATACGTGATGGATCACCCGGAGTTGGCGACCCAGTTTTTACTTTACGGCAGCCTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGAc                                                                                       >  1:116642/3‑233 (MQ=255)                                                                                                                                         ntGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGGGGCGCATCATGTGCGCGTTCAAGATGATGACGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTATACTTCGTGAAATACGTGATGGATCACCCGGAGTTGGCGAACCAGTTTTTACTTTACGGCAGCCTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGatcat                                                             >  1:196092/2‑251 (MQ=255)                                                                                                                                            gCGAAAGCCCTTAAGCGGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGACGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTGCGTGAAATACGTGATGGATCACCCGGAGTTGGCGACCCAGTTTTTACTTTACGGCAGCCTCgn                                                                                                                                        <  1:63897/173‑2 (MQ=255)                                                                                                                                             cGAAAGACCTTAAGCTGCTGCTGGGCACCAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGACGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATACGTGATGGATCACCCGGAGTTGGCGACCCAGTTTTTACTTTACGGCAGCCTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGAc                                                                                       <  1:874/221‑1 (MQ=255)                                                                                                                                                                                 ggCGCATCATGTGCGCGTTCAAGATGATGACGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATACGTGATGTATCACCCGGAGTTGGCGACCCAGTTTTTACTTTACGGCAGCCTCTCCACCAGGTTCGGCTCGCTTTGCTCCTCTCGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCAGCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCAcc                     <  1:170888/251‑1 (MQ=255)                                                                                                                                                                                                cgTTCAAGAGGATGACGACCTGCGCCAACGTGGTGCGCGGCGGGGGGACGCTCTACTTCGTGAAATACGTGATGGAGCACGCGGAGTTGGCGACCCAGTTTGTACTTTACGGCAGCCTCGCCACCATGTTCGGCTCGCTTTGCTCCTCTCGCTTGCTGGGCCGCTTCGACCGCGTCAACGCCTGCAATTGGATCATCGTCGCCTTCTCGCTGAt                                           <  1:302615/214‑1 (MQ=255)                                                                                                                                                                                                    aaaGATGATGACGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATACGTGATGGATCACCCGGAGTTGGCGACCCAGTTGTTACTTTACGGCAGCCTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCGTCAAGTGGATCAGCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCACATCgcgc  <  1:112207/250‑1 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 CATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATACGTGATGGATCACCCGGAGTTGGCGACCCAGTTTTTACTTTACGGCAGCCTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCACATCGCGC  >  NC_000913/285904‑286349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑