| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 694,841 | C→T | E39K (GAA→AAA) | miaB ← | isopentenyl‑adenosine A37 tRNA methylthiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 694,841 | 0 | C | T | 100.0% | 25.0 / NA | 10 | E39K (GAA→AAA) | miaB | isopentenyl‑adenosine A37 tRNA methylthiolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (4/6); total (4/6) | |||||||||||
CATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGTGCTCGCCTTCTTGCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATG > NC_000913/694614‑695032 | catcTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGTGCTCGCGTTCTTGCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTGTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTTCGCCACGTCGGTCAGTTGATAGc < 1:241454/251‑1 (MQ=255) ggCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGTGCTCGCCTTCTTGCGATGCCACGCAGCCACCGCCGCCGATCATCAGGACTGGATTCTTCTCTTTTAACAGTTACCAGCGACCCAACTGATGGAAGACTTTTTCCTCAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTTCGCCACGCCGGTCAGTTGATAGCCgtgggt > 1:183313/1‑251 (MQ=255) cccAAAAATAATATCGACATAGTGGGCGCGCTGGCGACTGTGCTCGCCTTCTTGCGATGCCACGCCGCAACCGACGCCGATAATCAGGTCTGGCTTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTTCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCt > 1:211552/1‑251 (MQ=255) gATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTTCGCCACGTCGGTCAGTTGATAGCCgtgggtgg > 1:105667/1‑175 (MQ=255) gCCACGCAGCCACCGACGCCGATAAGCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCGTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTTCGCCACGTCGGGCAGTTGATAGCCGTGGGGGACATCCAGCAAAGCGGCCATCTGCGATGAATCGTACTCGTCCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTgg < 1:305351/250‑1 (MQ=255) cAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTGCAGCAGCAGCACATCCGCTTCTTTCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCg < 1:149519/121‑1 (MQ=255) ttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTTCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATg < 1:118652/250‑1 (MQ=255) gNCTNCTCGCNGATTNAGCAGGTGTTCAGCANCAGCACACCCGCTTCTTTCNNCNCCTCGNTCAGt < 2:129213/66‑1 (MQ=255) cgGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTTCGCCACGTCGGTCAGTTGATAGTCGTGGGTGGCATTCAGCAGATCGGCCATCTTAGATGAATCGTACTCGTTCATCTGAACGCCCCAGGTTTTAATCTGGAGTTTTTCGGTCATCGACTTGCTCTTGc > 1:322031/1‑169 (MQ=255) gATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTTCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTTCTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGgcgc < 1:118384/194‑1 (MQ=255) | CATCTCCGGCAGACGGTGCAGCGTTTGCGGCCCAAAAATAATATCGACATAGTGGGCGCGCTGGCGAATGTGCTCGCCTTCTTGCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATG > NC_000913/694614‑695032 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |