Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,715,932	C→T	intergenic (‑43/+16)	pdxY ← / ← tyrS	pyridoxal kinase 2/tyrosine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,715,932	0	C	T	100.0%	24.4 / NA	10	intergenic (‑43/+16)	pdxY/tyrS	pyridoxal kinase 2/tyrosine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/6);  total (4/6)

GCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTAATGGTGATGGCATTGGAGGCGATAGTTTTACGTGCCTGACCACG  >  NC_000913/1715687‑1716109                                                                                                                                                                                                                                                      |                                                                                                                                                                                  ctgtatcgttGCACCATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCACAGGCGGCGCATCGGAAACTCTGCCGCACGGTCTCCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCTTTTcc                                                                                                                                                                              >  1:325553/9‑251 (MQ=255)              caATTTCGGTTAAATGGATGGGCGGCATCACGCAGCCAGGCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAACGGCGTTCAGCGGCCAGACGTTCGCGCCCCGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCCTGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCTTTTCCACTTAATGCatt                                                                                                                                                                  >  1:77625/1‑250 (MQ=255)                                                        aTTTGCCGTATTGGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTTGATAGGGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCGCTCTCCTTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTTCCGCGACGCAg                                                                                                                       <  1:269871/251‑1 (MQ=255)                                                                                                                  gcCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACAATAAACAACGTGAGACTTGATAGCGAGAATATGTTTCATCATGTTCCCTGTATAAAATCCAGGGGGAGTGATGTCTCACTCCCCTTTTCCACTTAAGGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACcg                                                                                                                               <  1:179529/185‑1 (MQ=255)                                                                                                                                     aaCTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCTTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCGTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGtttn                                                       <  1:204771/238‑2 (MQ=255)                                                                                                                                     aaCTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCTTTTCCACTTAATGCATTATNTCCNNNNNAtnnnnnnnnnn                                                                                                                                           >  2:204771/1‑144 (MQ=255)                                                                                                                                                   gTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCTTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAATCAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTGTGTCACCGTTAATGGTTATGGCATTGGa                             <  1:275548/250‑1 (MQ=255)                                                                                                                                                                ccATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCTTTTCCACTTAATGCAATATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTGTCACCGTTAATGGTGATGGCATTGGAGGCGATAGTTTTAc               <  1:318206/251‑1 (MQ=255)                                                                                                                                                                                         cGAGAATATTTTTCATCATGTTCCCTGTATACAAACCAGGGGGAGTGATTTCTCACTCCCCTTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTAATGGTGATGGCATTGGAGGCGATAGTTTTACGTACCTGACCACg  >  1:102035/1‑239 (MQ=255)                                                                                                                                                                                                                                             tcACTCCCCTTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTAATGGTGATGGCATTGGAGGc                          <  1:48745/163‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                  GCAATGCCTTGCACAATTTCGGTTAAATGGCTGGGCGGCATCACGCAGCCAGTCCATTTGCCGTATTGGGTGTGATTAGAAAATTGAACGGTGTTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTAATGGTGATGGCATTGGAGGCGATAGTTTTACGTGCCTGACCACG  >  NC_000913/1715687‑1716109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑