Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	286,492	G→A	L366L (CTG→CTA)	yagG →	putative D‑xylonate transporter YagG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	286,492	0	G	A	100.0%	31.2 / NA	12	L366L (CTG→CTA)	yagG	putative D‑xylonate transporter YagG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/6);  total (6/6)

CGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGG  >  NC_000913/286273‑286736                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     cGCCTTCAAGCGGATCATAGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGCGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTATTCAGCCTGAAGATTGGCCTGGCGATTggcg                                                                                                                                                                                                                       >  1:85910/1‑251 (MQ=255)                         cTACTCGCTGATCAGACTGCTGATTTTCGTCACCCCGGCAGAGCACATCGCAATCATTTTCACCCTCAACATCCTGTTCCTGTTCGGCTTTAATACCACCACGCCGATGCAGTGGCTGATGCCGTCTGACGTGGGGGCCTACGCGGGGAACCGCAGCGATCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTATTCAGCCTGAAGATTGGCCt                                                                                                                                                                                                                                  >  1:169399/1‑216 (MQ=255)                                 tGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGAAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTATTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGGGGGGGGccgggacccggcgtcactc                                                                                                                                                                                       >  1:292611/1‑233 (MQ=255)                                                                                                                                                               tggACTACGAGGAGAGCCGCAGCGGTCGCCGCCGCGACGGGCTGGTCTTCTCCACCTACCTATTCAGCCTGAAGATGTGCCTGGCGATGGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTGGACGTGCTcacc                                                                                                                                          <  1:104256/170‑1 (MQ=255)                                                                                                                                                                   cTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTATTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCCGGATCCTGGCGTCCGTCAACTCTTCAGACAGCCGCAGCGTGCAGACGGTTGAGGGGCTCACCACCATCAAAATTCTGTTCCGCGTGGTGCCGGGGGGGCTCTACGCGGGCATGTTCATCATGCTGGCGCTTTTCAAGCTCCCCgcgg                                                     >  1:270701/1‑248 (MQ=255)                                                                                                                                                                              gCCGCAGCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTATTCAGCCGGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTcaccacn                                                                                                                                       <  1:65661/158‑2 (MQ=255)                                                                                                                                                                                      ggTCTCCGCCTCGGCGGGCTGGTTTTCTCCACCTACCTATTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGc                                                                                                                                                                                                         <  1:45978/84‑1 (MQ=255)                                                                                                                                                                                          gccgccTCGACGGGCTGGTGTTCTCCACCTACCTATTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCtn                                                                                                                                                                                                       <  1:192585/82‑2 (MQ=255)                                                                                                                                                                                             gccTCGACGGGCTGGTGTTCTCCACCTACCTATTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCgn                                                                       <  1:84292/207‑2 (MQ=255)                                                                                                                                                                                                 cGACGGGCTGGTGTTCTCCACCTACCTATTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGGGGGGTGGATACTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGGTGAGGTGCTCACCACCCTCCAAATTCTGTTCTGCGTGGTGCCGGTGGGGCTCTACGCgggg                                                                                          >  1:274477/1‑183 (MQ=255)                                                                                                                                                                                                   aCGGGCTGGTGTTCTCCACCTACCTATTCAGCCTGAAGATTGGCCTGGCGATTGGTGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAGTCTGTTCTGCTTGGTGCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAg                                                               <  1:154297/209‑1 (MQ=255)                                                                                                                                                                                                                      ctacctATTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGGGGTGGGCTGGATACTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGGCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAACTCCCCGATGCCCGCGGGGAGGCCATCAGCCGGCAGCTGGTTAAGCACcgcgggggcgatc  >  1:1065/1‑242 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     CGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGG  >  NC_000913/286273‑286736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑