Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,498,660	C→T	P278L (CCC→CTC)	cysG →	siroheme synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,498,660	0	C	T	100.0%	57.4 / NA	21	P278L (CCC→CTC)	cysG	siroheme synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/11);  total (10/11)

AGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCGGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACCAC‑TGCGTACCCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTG‑GTGG‑CGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGC  >  NC_000913/3498444‑3498868                                                                                                                                                                                                                          |                                                                                                                                                                                                                   aGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCGGGCTGCTGACACTGAAAGGACTGCCACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGa                                                                                                                                                                                   >  1:10487/1‑250 (MQ=255)                             cgGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCGGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAGGCAAACGCGTGGTGCGGc                                                                                                                                                      <  1:6303/251‑1 (MQ=255)                                       gtgCTGGTTGGGGCAGGTCCGGGCGATGCCGGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGGACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAGGCAAACGCGTGGTGCGGCTGAAAGGTgg                                                                                                                                            <  1:126050/251‑1 (MQ=255)                                         gCTGGTTGGTGCAGGTCCGGGCGATGCCGGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCg                                                                                                                                          <  1:103957/251‑1 (MQ=255)                                                                  gCCGGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCTTTTATTTTTGGCCGtg‑gtgg                                                                                                                <  1:271889/251‑1 (MQ=255)                                                                   ccGGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAgg                                                                                                                                                                       >  1:248696/1‑196 (MQ=255)                                                                   ccGGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAgg                                                                                                                                                                       >  1:248690/1‑196 (MQ=255)                                                                                                      cagcagGCAGATGTG‑TGGTCTACGACCGTCGGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGTTGGATGCCAC‑TCCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAg                                                                                                                                                                                 <  1:183527/150‑1 (MQ=255)                                                                                                        gcaggcagATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTG‑GTGG‑CGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGt                                                                         >  1:256377/1‑251 (MQ=255)                                                                                                                            aCGACCGTCTGGTTTCTGACGATATTATGAATCTGGGACGCCGCGTTGCGGACCGTGTTTTCGTCCGCATAAGTGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCGACGGGAAGCGCAAAAAGGCAAACGCTTTTTGCTGCTGAAAGGTGGCGATCCTTTTATTTTTGGCCGTG‑‑TGGACGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGt                                                        <  1:240658/248‑1 (MQ=255)                                                                                                                                      ggTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGGGTTTTCGTCGGCAAACGCTCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCGGCTGCGGGAAGCGCAAAAAGTCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTGTATTTTTGGCC‑TGTGTGG‑CGAAGAGCTGTAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGTGTATTACCGCAg                                              <  1:233467/248‑1 (MQ=255)                                                                                                                                               cGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTCTCGTCGGCAAAAGCGCGGGATAC‑ACATGCGTAATCCAGGAGGAGACTAACCAGATc                                                                                                                                                                                              >  1:154862/1‑97 (MQ=255)                                                                                                                                                              ggTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTG‑GTGG‑CGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCggg                     <  1:158511/249‑1 (MQ=255)                                                                                                                                                               gTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACAGC‑TGCGTACTCCCGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAACAGGCCAACGCGTGGAGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTG‑GTGG‑CGAAGAGCTGGaaa                                                                                                 >  1:174446/1‑172 (MQ=255)                                                                                                                                                                 aCGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTG‑GTGG‑CGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTAttn                <  1:201665/251‑2 (MQ=255)                                                                                                                                                                     cgcgATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACCAC‑TGCGTCCTCAAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAACAGGCAAACGCGTGGTGCGGCTGCAAGGTGGCGATCCGTTTATTTTTGGCCGTG‑GTGG‑CGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGcc                             >  1:154526/1‑234 (MQ=255)                                                                                                                                                                                taacgccatcgtcgGCAAACGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTG‑GTGG‑CGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATcgn  <  1:194271/242‑2 (MQ=255)                                                                                                                                                                                             ggCAAACGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTT‑GGCCGTG‑GTGG‑CGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCggg                     >  1:252044/1‑217 (MQ=255)                                                                                                                                                                                               cAAACGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCACAAAAAgg                                                                                                                                                                       >  1:162802/1‑72 (MQ=255)                                                                                                                                                                                                 aaCGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAGGCaaa                                                                                                                                                                   >  1:24805/1‑74 (MQ=255)                                                                                                                                                                                                 aaCGCGCGGGATACCAC‑TGCGTACTCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAGGCaaa                                                                                                                                                                   <  2:24805/74‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                   AGTTAATCAACGAACCGCTCGACCATCGCGGTGAAGTGGTGCTGGTTGGTGCAGGTCCGGGCGATGCCGGGCTGCTGACACTGAAAGGACTGCAACAAATTCAGCAGGCAGATGTGGTGGTCTACGACCGTCTGGTTTCTGACGATATTATGAATCTGGTACGCCGCGATGCGGACCGTGTTTTCGTCGGCAAACGCGCGGGATACCAC‑TGCGTACCCCAGGAAGAGATTAACCAGATCCTGCTGCGGGAAGCGCAAAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTG‑GTGG‑CGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGC  >  NC_000913/3498444‑3498868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑