Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,487,627	C→T	R279R (CGC→CGT)	yhfK →	putative transporter YhfK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,487,627	0	C	T	100.0%	80.1 / NA	28	R279R (CGC→CGT)	yhfK	putative transporter YhfK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/11);  total (17/11)

CCAGCAAAAAGCGGTCGATCTAATTACCCAGTGCTATCAGCAAATGCATATGCTTTCCGCGCAAAATAATACTGACTACAAGCGGATGCTGCGTATTTTCCAGGAGGCGCTGGATTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGCCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGACTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTAT  >  NC_000913/3487399‑3487870                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    ccAGCAAAAAGCGGTCGATCTAATTACCCAGTGCTATCAGCAAATGCATATGCTTTCCGCGCAAAATAATACTGACTACAAGCGGATGCTGCGTATTTTCCAGGAGGCGCTGGATTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCtgat                                                                                                                                                                                                                                   >  1:77485/1‑247 (MQ=255)  cAGCAAAAAGCGGTCGATCTAATTACCCAGTGCTATCAGCAAATGCATATGCTTTCCGCGCAAAATAATACTGACTACAAGCGGATGCTGCGTATTTTCCAGGAGGAGATGGATTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTgcg                                                                                                                                                                                                                                                >  1:311679/1‑233 (MQ=255)           gCGGTCGATCTAATTACCCAGTGCTATCAGCAAATGCATATGCTTTCCGCGCAAAATAATACTGACTACAAGCGGATGCTGCGTATTTTCCAGGAGGCGCTGGATTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGAAATTCTTTAcc                                                                                                                                                                                                                      >  1:119870/1‑250 (MQ=255)              gTCGATCTAATTACCCAGTGCTATCAGCAAATGCATATGCTTTCCGCGCAAAATAATACTGACTACAAGCGGATGCTGCGTATTTTCCAGGAGGCGCTGGATTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCg                                                                                                                                                                                                                  >  1:125244/1‑251 (MQ=255)                    ntAATTACCCAGTGCTATCAGCAAATGCATATGCTTTCCGCGCAAAATAATACTGACTACAAGCGGATGCTGCGTATTTTCCAGGAGGCGCTGGATTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGcc                                                                                                                                                                                                            >  1:192847/2‑251 (MQ=255)                                  cTATCAGCAAATGCATATGCTTTCCGCGCAAAATAATACTGACTACAAGCGGATGCTGCGTATTTTCCAGGAGGCGCTGGATTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACg                                                                                                                                                                                               >  1:1320/1‑250 (MQ=255)                                         cAAATGCATATGCTTTCCGCGCAAAATAATACTGACTACAAGCGGATGCTGCGTATTTTCCAGGAGGCGCTGGATTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGaaa                                                                                                                                                                                        >  1:129165/1‑250 (MQ=255)                                                    gCTTTCCGCGCAAAATAATACTGACTACAAGCGGATGCTGCGTATTTTCCAGGAGGCGCTGGATTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCTGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGgcg                                                                                                                                                                            >  1:319929/1‑251 (MQ=255)                                                           gcgnANNANNATANTGACGACAAGCGGATGCTGCGNNTTTTCCAGGNGGCGGTGGATTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGAAGAGGNGCAAAAGCTNNGCNAGCGGNGNNNTNNNGANGAAGTTATCCGCTGGAATGNGCNNACCGTCNCCGCTCGTCTGCGCGTGCTGGCtgat                                                                                                                                                                                                                                   <  2:77485/189‑1 (MQ=255)                                                                                                     naggaggCGCTGGATTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGACGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAAtt                                                                                                                           >  1:58320/2‑251 (MQ=255)                                                                                                     cAGGAGGCGCTGGATTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGGCGCACTGGCAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAAtt                                                                                                                           >  1:20613/1‑251 (MQ=255)                                                                                                           gcgcTGGATTTACAGGAACATATTTCNGTCAGGNNGCNTCAGCCGGAAGAGGTGCAANAGNNNGNNNANAGTAGNCANNCGGAAGAAGNTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGNCATTCTTTANNATCGCCTGCCAACGCGTTTTANGATNNANNANCaanatn                                                                                                                                                                               >  2:70704/1‑189 (MQ=255)                                                                                                              cTGGATTTACAGGCACATCTTTCGGTCAGTTTGCATCAGCAGGAAGAGGTGCAAAAGCTGGTCGTGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGc                                                                                                                       >  1:72386/1‑246 (MQ=255)                                                                                                                                  gtCGGTCAGTTGGCAGCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCAGGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCGCGTCGGCGCGTGCTGGCTGAGGACATTCGTTACCATCGCTTGCCAACGCGTTTTACGAGGGAAAAGTAAATTGGCGCACTGGAAAAAATCGGCCGCCAGCATGCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCgac                                                                                               <  1:315515/249‑3 (MQ=255)                                                                                                                                    cGGTCAGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGTGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGGCGCACTGGAAAAAATcgn                                                                                                                                                            <  1:195689/187‑2 (MQ=255)                                                                                                                                     ngTCAGTTTGCATCAGACGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCcgcg                                                                                           >  1:82048/2‑251 (MQ=255)                                                                                                                                        cAGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCTTGCCAACGCGTTTTACGAGGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGTATAATCCGGTTGGGCAATGCTGCTACTGGCATTTCAGCCGCATCGCCCGCGtg                                                                                         <  1:133745/250‑1 (MQ=255)                                                                                                                                         aGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGAAA‑GCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGtgctg                                                                                      <  1:286274/251‑1 (MQ=255)                                                                                                                                          gTTTGCATCAGCCGGAAGAGGTGAAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGGGCGGGCTGATGACATTCTTGACCATCGCCTGCCAACGCTTTTTACGATGGAAAATCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGc                                                                                                                       <  1:42540/218‑1 (MQ=255)                                                                                                                                                           gngGTNCAAANGCNGGTCGAGCGTAGCCANGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGNNTNNNCNTgctggct                                                                                                                                                                                                                                      <  2:228558/90‑1 (MQ=255)                                                                                                                                                           gagGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGACTGCGCACACCGTCGCCGGTCGTCTGAGCGCgatggct                                                                                                                                                                                                                                      >  1:228558/1‑90 (MQ=255)                                                                                                                                                                                agTAGCTATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCTTTTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCGGGCCAACGCGTATTACGATGGAAAAGCAAATGGGCGCACTGGAAAAAAGCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCTGTGACTTACTGGc                                                <  1:326397/250‑1 (MQ=255)                                                                                                                                                                                    gCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCt                                                                                                                      <  1:290883/177‑1 (MQ=255)                                                                                                                                                                                    gCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACAGTCGCCGCTCGTCTGCGTGTGCTGGCTGATGAAAATCTTTACCATCGTCTGACACCGCGTCTTACGATGGCGCAGCAAATTGGCGCGCTGGAAACAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGACGCATCGCCCGCGTGCTGCGCACCCAACAACCGCTCTATCCCCGTGCCTTACTGGCCGAt                                            >  1:158718/1‑251 (MQ=255)                                                                                                                                                                                    cccatgcgtaataatgtATCCGCTTGAAGGCGCAATCCGGCCCCGCTCGTCGGCGCGTGCTGGCTGATGACATTCTTTCCCCTCGCCTCCCAACGCTTTTTACGAGGGAAAAGCAAATGCGCGCACTGGAAAAAATCGCCCGCCTGCTTCCGTATATGCCGTTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCTCCCAAAAACCGCTCTATGCCCGTGACTTACTGGCCGAt                                            <  1:131850/235‑1 (MQ=255)                                                                                                                                                                                           ggAAGAAGTTACCCGCGGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGTTTGGTCAATTCTGCTACTGTCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGACTTACTGGCCGATAAACAgc                                     <  1:231563/251‑1 (MQ=255)                                                                                                                                                                                                    tATCCNNTGGAATGCGCAAACCGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGaaa                                                                                                                                                                                        <  2:1320/95‑1 (MQ=255)                                                                                                                                                                                                                        ccGTCGCCGCTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATCATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGACTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGaa         >  1:289229/1‑250 (MQ=255)                                                                                                                                                                                                                              ccgcTCGTCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGGCGCACTGGACAAACTCGACCGCCAGCATCCGGATAATCCGGTTGGGCACTTCTGCTACTGGCATTTCAGCCGCATCGCCCGAGTGCTGCGCACCCACAAACCGCTCTATGCCCGTGACTTACTGGCCGATAAACAGCGGCGAATGCCATTAATTCCGGCGCTGAAAAGTTAt  >  1:40779/1‑251 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    CCAGCAAAAAGCGGTCGATCTAATTACCCAGTGCTATCAGCAAATGCATATGCTTTCCGCGCAAAATAATACTGACTACAAGCGGATGCTGCGTATTTTCCAGGAGGCGCTGGATTTACAGGAACATATTTCGGTCAGTTTGCATCAGCCGGAAGAGGTGCAAAAGCTGGTCGAGCGTAGCCATGCGGAAGAAGTTATCCGCTGGAATGCGCAAACCGTCGCCGCTCGCCTGCGCGTGCTGGCTGATGACATTCTTTACCATCGCCTGCCAACGCGTTTTACGATGGAAAAGCAAATTGGCGCACTGGAAAAAATCGCCCGCCAGCATCCGGATAATCCGGTTGGGCAATTCTGCTACTGGCATTTCAGCCGCATCGCCCGCGTGCTGCGCACCCAAAAACCGCTCTATGCCCGTGACTTACTGGCCGATAAACAGCGGCGAATGCCATTACTTCCGGCGCTGAAAAGTTAT  >  NC_000913/3487399‑3487870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑