Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,069,443 C→T intergenic (‑167/+32) yihQ ← / ← yihR sulfoquinovosidase/putative sulfoquinovose mutarotase YihR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,069,4430CT93.3% 34.7 / NA 15intergenic (‑167/+32)yihQ/yihRsulfoquinovosidase/putative sulfoquinovose mutarotase YihR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (6/8);  minor base G (0/1);  total (6/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

AAAATGTAGGGTAAAACTGTCGTTATTCTGATGAAATTGAAAATCTAATAACTGTGGACGTGGCGTATCCATAACTAATCTCCATAGAGTTGAGAACGAGATTGTTGCCTTCCTGTTATTACAGGCTTATGTAAAATGATTCTCATAAAACTGATATATAAAAACTTTACAGAAGCTGAGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGTGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTT  >  NC_000913/4069205‑4069613
                                                                                                                                                                                                                                              |                                                                                                                                                                          
aaaaTGTAGGGTAAAACTGTCGTTATTCTGATGAAATTGAAAATCTAATAACTGTGGACGTGGCGTATCCATAACTAATCTCCATAGAGTTGAGAACGAGATTGTTGCCTTCCTGTTATTACAGGCTTATGTAAAATGATTCTCATAAAACTGATATATAAAAACTTTACAGAAGCTGAGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTTTTCAATGAtttt                                                                                                                                                                >  1:3282/1‑251 (MQ=255)
           tAAAACTGTCGTTATTCTGATGAAATTCAAAATCTAATACCTGAGGACGTGGCGTCTCCCTAACTCATCTCCATAGAGTTGAGAACGAGATTGTTGCCTTCCTGTTATTACAGGCTTATGTAAAATGCTTCTCATAAAACTGATATATAAAAACTTTACAGCAGCTGAGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTTTTCAATGATTTTg                                                                                                                                                               >  1:172010/1‑241 (MQ=255)
                   tCGTTATTCTGATGAAATTGAAAATCTAATAACTGTGGACGTGGCGTATCCATAACTAATCTCCATAGAGTTGAGAACGAGATTGTTGCCTTCCTGTTATTACAGGCTTATGTAAAATGATTCTCATAAAACTGATATATAAAAACTTTACAGAAGCTGAGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTTTTCAATGATTTTGACTGCTAATCAATACa                                                                                                                                               >  1:4950/1‑249 (MQ=255)
                    cGTTATTCTGATGAAATTGAAAATCTAATAACTGTGGACGTGGCGTATCCATAACTAATCTCCATAGAGTTGAGAACGAGATTGTTGCCTTCCTGTTATTACAGGCTTATGTAAAATGATTCTCATAAAACTGATATATAAAAACTTTACAGAAGCTGAGATGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTGTAGCGAGTGAAATGTTTTTCAATGATTTTGACTGCTAATCAATACAAGt                                                                                                                                            <  1:140144/251‑1 (MQ=255)
                     gTTATTCTGATGAAATTGAAAATCTAATAACTGTGGACGTGGCGTATCCATAACTAATCTCCATAGAGTTGAGAACGCGATTGTTGCCTTCCTGTTATTACAGGCTTATGTAAAATGATTCTCATAAAACTGATATATAAAAACTTTACAGAAGCTGAGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTTTTCAATGATTTTGACTGCTAATCAATACAAGtt                                                                                                                                           >  1:156807/1‑251 (MQ=255)
                                  aaTTGAAAATCTAATAACTGTGGACGTGGCGTATCCATAACTAATCTCCATAGAGTTGAGAACGAGATTGTTGCCTTCCTGTTATTACAGGCTTATGTAAAATGATTCTCATAAAACTGATATATAAAAACTTTACAGAAGCTGAGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTTTTCAATGAGTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTcn                                                                                                                              <  1:65991/251‑2 (MQ=255)
                                                taaCTGTGGTCTTTGCGTATCCATAACGAATCACCATATAGTTGATAACGCGATTGTTGCCTTCCTGTTATTACATGCTTATGTAAAATGATTCTCAGAAAACTGATATATAAAAACTTTACAGAAGCTGAGCTGTCTTTATCTTAAGAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTTTTCAATGAttt                                                                                                                                                                 <  1:150852/202‑1 (MQ=255)
                                                        gACGTGGCGTATCCATAACTAATCTCCATAGAGTTGAGAACGAGATTGTTGCCTTCCTGTTATTACAGGCTTATGTAAAATGATTCTCATAAAACTGATATATAAAAACTTTACAGAAGCTGAGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTTAAATGTTTTTCAATTATTTGGACGGCTAATCAATACAAg                                                                                                                                             <  1:116411/214‑1 (MQ=255)
                                                                                 ccATAGAGTTGAGAACGAGATTGTTGCCTTCCTGTTATTACAGGCTTATGTAAAATGATTCTCATAAAACTGATATATAAAAACTTTACAGAAGCTGAGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTTAAATGTTTTTCAATGATTTTGTCTGCTAATCAATGCAAGTTATTTTCTCTGTCCGTAAATAGTAAAAAACAGGCGGTGCGGTTTTCCTGATTCCAGta                                                                                  <  1:274554/248‑1 (MQ=255)
                                                                                                         tgCCTTCCTGTTATTACAGGCTTATGTAAAATGATTCTCATAAAACTGATATATAAAAACTTTACAGAAGCTGAGTTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTGTGCAATGATGGTGACTGCGAATCAATACAAGGTATTTTCGCTGTCCGTAAAGATTAAAAAACAGGCGGTGCGGTGTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAg                                                        <  1:147833/250‑1 (MQ=255)
                                                                                                                        aCAGGCTTATGTAAAATGATTCTCATAAAACTGATATATAAAAACTTTACAGAAGCTGAGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTTTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGTGCGGTTTGCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAAc                                        <  1:289052/251‑1 (MQ=255)
                                                                                                                                               cATAAAACTGATATATAAAAACTTTACAGAAGCTGAGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTTTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGTGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTccc                 <  1:165568/251‑1 (MQ=255)
                                                                                                                                                 naaaaCTNNTATATAAAAACTTTACAGAAGCTGGGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGtttttc                                                                                                                                                                         <  2:23110/96‑1 (MQ=255)
                                                                                                                                                             ataAAAACTTTACAGAAGCTGAGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTTTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGTGCGGTTTTCCTGATTCCAGTAATAATAAGTc                                                                        >  1:152049/1‑182 (MQ=255)
                                                                                                                                                              taAAAACTTTACAGAAGATGAGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTTTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTTTGTCCGTAAATATTAAAAAACAGGCGGTGCGGTTTTCCTGATTCCAGTAATACTAAGTCGATACCTGAATTAAAAGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAAtttt  >  1:358/1‑251 (MQ=255)
                                                                                                                                                                               ctgagctgTTTTTAACTGAATAAATTGAAGATACGTAATGTTGCCTTTAGCGAGTGAAATGATTTTCAATGATCTTGACTGGGAATCAATACGAGTTATTGTCTCTGTCCGTAAATATTAAAAAACAGGCGGTGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAACTAAAGGCGTGAGGCGGGCAACTCATAGGTTCAACTGc                        <  1:124879/212‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                          
AAAATGTAGGGTAAAACTGTCGTTATTCTGATGAAATTGAAAATCTAATAACTGTGGACGTGGCGTATCCATAACTAATCTCCATAGAGTTGAGAACGAGATTGTTGCCTTCCTGTTATTACAGGCTTATGTAAAATGATTCTCATAAAACTGATATATAAAAACTTTACAGAAGCTGAGCTGTTTTTAACTTAATAAATTGAAGATACATAATGTTGCCTTTAGCGAGTGAAATGTTCTTCAATGATTTTGACTGCTAATCAATACAAGTTATTTTCTCTGTCCGTAAATATTAAAAAACAGGCGGTGCGGTTTTCCTGATTCCAGTAATAATAAGTCGATACCTGAATTAAAGGCGTTAGGCGGGCAACTCATAGGTTCAACTGCCAGTCCCTGACGCTGTAATTTT  >  NC_000913/4069205‑4069613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑