Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 629,709 G→A G137E (GGA→GAA)  entH → proofreading thioesterase in enterobactin biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913629,7090GA83.3% 20.8 / ‑2.6 12G137E (GGA→GAA) entHproofreading thioesterase in enterobactin biosynthesis
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (7/3);  total (9/3)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCTCTATGGACACGCACACGGATAACAACTATGAACAAATCAGGGAAATACCTCGTCTGGACAGTGCTCTCTGTAATGGGAGCATTTGC  >  NC_000913/629469‑629952
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   
cgTCGGCGGCGCTGTCAGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGAACGTGCGTGGGAGGCACCGCACTTAATGCAACACACAATCGCCCGGTGTCTGCGGGAAAGGCACGCGGCGTCTCCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGAAGTTTTGGGATGAACCCg                                                                                                                                                                                                                                            >  1:46970/1‑250 (MQ=255)
                                                   cccGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGAATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGtt                                                                                                                                                                                         >  1:21856/1‑250 (MQ=255)
                                                                gtgtgtGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGAATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaagtt                                                                                                                                                                           >  1:91132/1‑251 (MQ=255)
                                                                                               cacCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGAATGAACCCGACAGACTTAGCTGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTACAACTCCGATTTACATGGt                                                                                                                                            >  1:329721/1‑251 (MQ=255)
                                                                                                                                              gCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGACCAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGCGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTa                                                                                              >  1:257405/1‑250 (MQ=255)
                                                                                                                                               cTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGAATGAACCCGACAGCATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACACAATGTAAc                                                                                            >  1:224244/1‑251 (MQ=255)
                                                                                                                                                            ngCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGAATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCTCTATGGac                                                                                >  1:38788/2‑250 (MQ=255)
                                                                                                                                                            ggCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGAATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGTTTTCAACTCCGATTTACATGGTTGCtg                                                                                                                                       <  1:273975/195‑1 (MQ=255)
                                                                                                                                                                                                            gTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGAATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCTCTATGGACACGCACACGGATAACAACTATGAACAAATCAgg                                               <  1:12959/235‑1 (MQ=255)
                                                                                                                                                                                                                  gcACTTGTCGGCTGGGTACGGCAGTTTTGGAATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCTCTATGGACACGCACACGGATAACAACTATGAACAAATCAGGGAAATACCTCGTCTGGACAgn                          <  1:32397/250‑2 (MQ=255)
                                                                                                                                                                                                                    ncTTGTCGGCTGGGTACGGCAGTTTTGGAATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTcc                                                                                                                                                        >  1:66315/2‑122 (MQ=255)
                                                                                                                                                                                                                                         gTTTTGGAATGAACCCGACAGAAATAGATGAGATTGCAGGAAAACTAGGTTAACGGAGAGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGGGTTGTTAAATTGTCCAAGGATGTTATAGAAACAAAATGGAACATCTCTAGGGACACGCACACGGATAACAACTATGACAAAATCAGGGAAATACCACGTCTGGACAGTGCTCTCTGTACTGGGAACATTTGc  >  1:26683/1‑251 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   
CGTCGGCGGCGCTGGCGGAAACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCTCTATGGACACGCACACGGATAACAACTATGAACAAATCAGGGAAATACCTCGTCTGGACAGTGCTCTCTGTAATGGGAGCATTTGC  >  NC_000913/629469‑629952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑